Plant Editosome Database
a curated database of RNA editosome in plants

SPL42 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: SPL42
Synonym: SPOTTED LEAF42
Description: Multiple genes related to chloroplast development and chlorophyll biosynthesis were significantly down-regulated, whereas many genes involved in leaf senescence, ROS production, and defense responses were upregulated in the spl42 mutant
Protein Family: Porphobilinogen deaminase
Subclass: NA
Construct Structure: NA
Gene ID & Species: Os02g0168800 (Oryza sativa)
Edited Gene(s): atpA    ndhD    ndhF    rpoB    rps14    rpoC2
Editing Type(s): C-to-U (12)
Publication(s): [1] Isolation and Characterization of Encoding a Porphobilinogen Deaminase in Rice., Plants (Basel, Switzerland), 2023. [PMID=36679117]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa Os02g0168800 Chloroplast atpA 1148 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTControlControlNo mutantNormalNANASequencing the RT-PCR product100.00%CompleteNone36679117
Ningjing6spl42The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6NANAA reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild typeNANASequencing the RT-PCR product71.00%HighDecreased36679117
Oryza sativa Os02g0168800 Chloroplast ndhD 878 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTControlControlNo mutantNormalNANASequencing the RT-PCR product100.00%CompleteNone36679117
Ningjing6spl42The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6NANAA reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild typeNANASequencing the RT-PCR product74.00%HighDecreased36679117
Oryza sativa Os02g0168800 Chloroplast ndhF 62 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTControlControlNo mutantNormalNANASequencing the RT-PCR product86.00%HighNone36679117
Ningjing6spl42The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6NANAA reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild typeNANASequencing the RT-PCR product41.00%MediumDecreased36679117
Oryza sativa Os02g0168800 Chloroplast rpoB 560 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTControlControlNo mutantNormalNANASequencing the RT-PCR product40.00%MediumNone36679117
Ningjing6spl42The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6NANAA reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild typeNANASequencing the RT-PCR product20.00%LowDecreased36679117
Oryza sativa Os02g0168800 Chloroplast rpoC2 NA CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTControlControlNo mutantNormalNANASequencing the RT-PCR product92.00%HighNone36679117
Ningjing6spl42The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6NANAA reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild typeNANASequencing the RT-PCR product72.00%HighDecreased36679117
Oryza sativa Os02g0168800 Chloroplast rps14 80 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTControlControlNo mutantNormalNANASequencing the RT-PCR product81.00%HighNone36679117
Ningjing6spl42The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6NANAA reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild typeNANASequencing the RT-PCR product53.00%MediumDecreased36679117
Last update: Jul 2021 (version 1.0)