SPL42 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

Os02g0168800

Chloroplast

atpA

1148

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	NA	NA	Sequencing the RT-PCR product	100.00%	Complete	None	36679117
Ningjing6	spl42	The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6	NA	NA	A reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild type	NA	NA	Sequencing the RT-PCR product	71.00%	High	Decreased	36679117

Oryza sativa

Os02g0168800

Chloroplast

ndhD

878

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	NA	NA	Sequencing the RT-PCR product	100.00%	Complete	None	36679117
Ningjing6	spl42	The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6	NA	NA	A reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild type	NA	NA	Sequencing the RT-PCR product	74.00%	High	Decreased	36679117

Oryza sativa

Os02g0168800

Chloroplast

ndhF

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	NA	NA	Sequencing the RT-PCR product	86.00%	High	None	36679117
Ningjing6	spl42	The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6	NA	NA	A reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild type	NA	NA	Sequencing the RT-PCR product	41.00%	Medium	Decreased	36679117

Oryza sativa

Os02g0168800

Chloroplast

rpoB

560

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	NA	NA	Sequencing the RT-PCR product	40.00%	Medium	None	36679117
Ningjing6	spl42	The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6	NA	NA	A reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild type	NA	NA	Sequencing the RT-PCR product	20.00%	Low	Decreased	36679117

Oryza sativa

Os02g0168800

Chloroplast

rpoC2

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	NA	NA	Sequencing the RT-PCR product	92.00%	High	None	36679117
Ningjing6	spl42	The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6	NA	NA	A reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild type	NA	NA	Sequencing the RT-PCR product	72.00%	High	Decreased	36679117

Oryza sativa

Os02g0168800

Chloroplast

rps14

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	NA	NA	Sequencing the RT-PCR product	81.00%	High	None	36679117
Ningjing6	spl42	The spl42 mutant was isolated from the ethyl methane sulfonate (EMS)-mutagenized japonica variety Ningjing6	NA	NA	A reddish-brown spotted leaf phenotype; The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes; The mature spl42 mutant plants displayed a significant reduction in the plant height, panicle length, flag leaf length, and seed setting rate compared with the wild type	NA	NA	Sequencing the RT-PCR product	53.00%	Medium	Decreased	36679117

Editing Factor:	SPL42
Synonym:	SPOTTED LEAF42
Description:	Multiple genes related to chloroplast development and chlorophyll biosynthesis were significantly down-regulated, whereas many genes involved in leaf senescence, ROS production, and defense responses were upregulated in the spl42 mutant
Protein Family:	Porphobilinogen deaminase
Subclass:	NA
Physical Interaction:	OsMORF8
Construct Structure:	NA
Gene ID & Species:	Os02g0168800 (Oryza sativa)
Edited Gene(s):	atpA ndhD ndhF rpoB rps14 rpoC2
Editing Type(s):	C-to-U (12)
Publication(s):	[1] Isolation and Characterization of Encoding a Porphobilinogen Deaminase in Rice., Plants (Basel, Switzerland), 2023. [PMID=36679117]

Summary

Editing Details