TRX z - Plant Editosome Database - CNCB-NGDC

TRX z - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	TRX z
Synonym:	PAP10; THIOREDOXIN PUTATIVE PLASTIDIC; TRX P; Thioredoxin z
Description:	OsTRX z protein physically interacts with OsMORFs in a redox-dependent manner and that the redox state of a conserved cysteine in the MORF box is essential for MORF-MORF interactions; wp2 and OsTRX z knockout lines show reduced editing efficiencies in many plastidial-encoded genes especially under high-temperature conditions; Results suggest that thioredoxin z regulates chloroplast RNA editing in plants by controlling the redox state of MORFs
Protein Family:	Thioredoxin (TRX)
Subclass:	NA
Physical Interaction:	MORF2; MORF8; MORF9; OsFLN1; OsFLN2
Construct Structure:	NA
Gene ID & Species:	LOC_Os08g29110 (Oryza sativa)
Edited Gene(s):	ndhA ndhB ndhD ndhF ndhG rpoB rpl2 rps8 ycf3 rps14 atpA
Editing Type(s):	C-to-U (184)
Publication(s):	[1] white panicle2 encoding thioredoxin z, regulates plastid RNA editing by interacting with multiple organellar RNA editing factors in rice, New Phytol, 2021. [doi:10.1111/nph.17047]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_Os08g29110

Chloroplast

1148

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	95.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	97.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	76.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	97.00%	High	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	85.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	82.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	79.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	87.00%	High	Similar	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

1070

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	88.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	90.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	53.00%	Medium	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	97.00%	High	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	41.00%	Medium	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	17.00%	Poor	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	17.00%	Poor	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	44.00%	Medium	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

473

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	26.00%	Low	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	77.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	11.00%	Poor	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	13.00%	Poor	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	75.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

563

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	74.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	78.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	3.00%	Poor	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	5.00%	Poor	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	69.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

1481

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	94.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	95.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	94.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	92.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	86.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	83.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	91.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

467

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	87.00%	High	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	77.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

586

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	81.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	57.00%	Medium	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	42.00%	Medium	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	88.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

611

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	85.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	69.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	26.00%	Low	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	17.00%	Poor	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	77.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

704

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

737

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	95.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	96.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	66.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	16.00%	Poor	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	10.00%	Poor	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	8.00%	Poor	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	30.00%	Low	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

830

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	79.00%	High	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	69.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

836

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	89.00%	High	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	82.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

878

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	89.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	90.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	68.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	96.00%	High	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	84.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	47.00%	Medium	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	40.00%	Medium	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	84.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

62

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	74.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	89.00%	High	Increased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	86.00%	High	Increased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	90.00%	High	Increased	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	78.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	87.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	84.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	82.00%	High	Similar	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

11

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	75.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	83.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	72.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	87.00%	High	Increased	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	49.00%	Medium	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	35.00%	Low	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	35.00%	Low	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	54.00%	Medium	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

347

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	86.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	94.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	66.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	96.00%	High	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	72.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	56.00%	Medium	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	52.00%	Medium	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	78.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

2

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	78.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	82.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	71.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	84.00%	High	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	65.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	68.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	67.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	57.00%	Medium	Similar	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

467

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	34.00%	Low	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	60.00%	High	Increased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	90.00%	High	Increased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	58.00%	Medium	Increased	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	56.00%	Medium	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	83.00%	High	Increased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	83.00%	High	Increased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	49.00%	Medium	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

545

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	41.00%	Medium	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	62.00%	High	Increased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	88.00%	High	Increased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	60.00%	High	Increased	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	79.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	91.00%	High	Increased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	91.00%	High	Increased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	73.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

560

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	40.00%	Medium	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	61.00%	High	Increased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	86.00%	High	Increased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	57.00%	Medium	Increased	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	78.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	88.00%	High	Increased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	88.00%	High	Increased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	72.00%	High	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

80

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	87.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	89.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	31.00%	Low	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	87.00%	High	Restored	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	35.00%	Low	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	22.00%	Low	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	24.00%	Low	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	36.00%	Low	Restored	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

182

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	90.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	94.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	94.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Increased	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	77.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	86.00%	High	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	85.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	74.00%	High	Similar	33119889

Oryza sativa

LOC_Os08g29110

Chloroplast

185

CDS

NA

NA

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 25℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 25℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 25°C	NA	10 days	Bulk sequencing of RT‐PCR products	97.00%	High	Similar	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	NA	NA	10 days	Bulk sequencing of RT‐PCR products	100.00%	Complete	Similar	33119889
Japonica cultivar Nipponbare	WT	Wild Type	Grown at 35℃	No mutant	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	96.00%	High	None	33119889
Japonica cultivar Nipponbare	wp2	Knockout	Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif	Knockout	Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	73.00%	High	Decreased	33119889
Japonica cultivar Nipponbare	L1	Knockout	Grown at 35℃; An "A" insertion	Knockout	Seedlings of the L1 mutant were albinic when grown at 35°C	NA	10 days	Bulk sequencing of RT‐PCR products	69.00%	High	Decreased	33119889
Japonica cultivar Nipponbare and IR36 (indica)	cp	Complementation	Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa	Knockout	Normal	NA	10 days	Bulk sequencing of RT‐PCR products	95.00%	High	Restored	33119889

Last update: Sep 2026 (version 2.0)