Tuning of nanoparticle biological functionality through controlled surface chemistry and characterisation at the bioconjugated nanoparticle surface.
Delyan R Hristov, Louise Rocks, Philip M Kelly, Steffi S Thomas, Andrzej S Pitek, Paolo Verderio, Eugene Mahon, Kenneth A Dawson
Author Information
Delyan R Hristov: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Louise Rocks: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Philip M Kelly: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Steffi S Thomas: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Andrzej S Pitek: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Paolo Verderio: Department of Biotechnology and Bioscience, University of Milano - Bicocca, Piazza dela Scienza, 3. Milan 20126, Italy.
Eugene Mahon: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Kenneth A Dawson: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
We have used a silica - PEG based bionanoconjugate synthetic scheme to study the subtle connection between cell receptor specific recognition and architecture of surface functionalization chemistry. Extensive physicochemical characterization of the grafted architecture is capable of capturing significant levels of detail of both the linker and grafted organization, allowing for improved reproducibility and ultimately insight into biological functionality. Our data suggest that scaffold details, propagating PEG layer architecture effects, determine not only the rate of uptake of conjugated nanoparticles into cells but also, more significantly, the specificity of pathways via which uptake occurs.
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Cell Line, Tumor
Coated Materials, Biocompatible
Humans
Materials Testing
Nanoparticles
Polyethylene Glycols
Surface Properties
Coated Materials, Biocompatible
Polyethylene Glycols
polyethylene glycol 1000
