FLO22 - Plant Editosome Database - CNCB-NGDC

FLO22 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	FLO22
Synonym:	FLOURY ENDOSPERM22
Description:	Mutation of FLO22 resulting in defective trans-splicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function; RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22 mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly up-regulated; FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing
Protein Family:	PPR
Subclass:	P
Physical Interaction:	DYW3
Construct Structure:	P
Gene ID & Species:	LOC_Os07g08180 (Oryza sativa)
Edited Gene(s):	nad9 rps7 rps4 rpl16 ccb6n nad3 cox1 cox2 orfX nad1 nad7 nad5 nad4
Editing Type(s):	C-to-U (135)
Publication(s):	[1] Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development., Journal of integrative plant biology, 2023. [PMID=36333887]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_Os07g08180

Mitochondrion

1286

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	97.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

1303

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	97.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	86.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

1318

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

1336

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	86.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

137

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	94.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	78.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

142

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	86.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	74.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

1450

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	96.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	80.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

1453

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	49.00%	Medium	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	25.00%	Low	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

151

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	72.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

38

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	91.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	77.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

98

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	87.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	76.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

1046

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	97.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

162

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	30.00%	Low	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	16.00%	Poor	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

537

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	35.00%	Low	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	78.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

155

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

190

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	71.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	57.00%	Medium	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

191

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	97.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	87.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

257

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	81.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

296

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

301

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	98.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	88.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

358

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	98.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	87.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

291

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	15.00%	Poor	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	30.00%	Low	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

120

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	4.00%	Poor	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	15.00%	Poor	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

134

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

2438

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	60.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	80.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

99

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	71.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

113

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	60.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	92.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	60.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

14

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	51.00%	Medium	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	35.00%	Low	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

167

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

UCG=>UUG
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	75.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	91.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	68.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

190

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	65.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	63.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

223

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	65.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	64.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

298

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

CCG=>UCG
NA=>NA

P=>S
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	70.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	68.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

311

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	65.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	66.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

328

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

CGG=>UGG
NA=>NA

R=>W
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	70.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	70.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

356

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	65.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	68.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

368

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

UCC=>UUC
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	60.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	92.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	59.00%	Medium	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

398

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	65.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	67.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

92

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	95.00%	High	None	36333887
Indica variety N22	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	65.00%	High	Decreased	36333887
Indica variety N22	COM-1	Complementation	A complementation test is performed by introducing fusion construct pCAMBIA1300-221- LOC_Os07g08180*3flag to flo22 mutant calli.	NA	All the positive transgenic plants restored seedling growth rate and plant height and produced transparent mature grains resembling those of the wild type.	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	Restored	36333887
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	90.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	64.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

127

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	97.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	83.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

155

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	64.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	74.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

205

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	87.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

215

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	99.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	84.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

468

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	43.00%	Medium	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	59.00%	Medium	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

91

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	100.00%	Complete	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	85.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

444

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	84.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	65.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

61

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	75.00%	High	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	60.00%	High	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

261

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	23.00%	Low	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	12.00%	Poor	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

729

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	30.00%	Low	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	18.00%	Poor	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

821

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	26.00%	Low	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	8.00%	Poor	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

867

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	21.00%	Low	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	6.00%	Poor	Decreased	36333887

Oryza sativa

LOC_Os07g08180

Mitochondrion

141

CDS

MSU7.0

MSU7.0

NA

NA

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Endosperm	9 DAF	Sequencing RT-PCR products	32.00%	Low	None	36333887
NA	flo22	A base substitution	A single base substitution of adenine (A) to thymine (T) at position 611 in LOC_Os07g08180 caused an amino acid change of histidine (His) to leucine (Leu).	NA	The plant height of the mutant was significantly less than the wild type at both the seedling and heading stages. The flo22 mutant exhibited slower dry matter accumulation during grain filling. There was no difference in the seed setting rate. Scanning electron microscopy (SEM) of mature grains showed that starch granules in mutant endosperm cells were loosely packed and rounder, whereas endosperm cells in the wild type were fully filled with compact starch granules. Iodine staining semithin sections of developing endosperm at 9 d after flowering (DAF) showed that the mutant had smaller SGs with lots of gaps compared with the wild type. Thousand-grain weight was significantly reduced by about 17%. Total starch and amylose contents in the mutant were significantly less than in the wild type, whereas lipid content was increased.	Endosperm	9 DAF	Sequencing RT-PCR products	13.00%	Poor	Decreased	36333887

Last update: Feb 2026 (version 2.0)