Plant Editosome Database
a curated database of RNA editosome in plants

PPR26 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: PPR26
Synonym: ZmPPR26
Description: Abolished editing at atpA-1148 causes a Leu to Ser change at AtpA-383 that leads to a reduction in the abundance of chloroplast ATP synthase in zmppr26; The accumulation of photosynthetic complexes are also markedly reduced in zmppr26, providing an explanation for the albino seedling-lethal phenotype
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: NA (Zea mays)
Edited Gene(s): atpA    ndhF    rpl20    rpl2    rpoC2    petB    rps8    ndhA
Editing Type(s): C-to-U (24)
Publication(s): [1] ZmPPR26, a DYW-type pentatricopeptide repeat protein, is required for C-to-U RNA editing at atpA-1148 in maize chloroplasts., Journal of experimental botany, 2021. [PMID=33929512]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays NA Chloroplast atpA 1148 CDS C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products100.00%CompleteNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products0.00%UneditedAbsent33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products100.00%CompleteRestored33929512
Zea mays NA Chloroplast ndhA 50 CDS C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products86.00%HighNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products59.00%MediumDecreased33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products83.00%HighRestored33929512
Zea mays NA Chloroplast ndhF 62 CDS C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products88.00%HighNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products26.00%LowDecreased33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products76.00%HighRestored33929512
Zea mays NA Chloroplast petB 668 CDS C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products84.00%HighNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products62.00%HighDecreased33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products81.00%HighRestored33929512
Zea mays NA Chloroplast rpl2 2 CDS C-to-U NA=>NA T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products68.00%HighNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products48.00%MediumDecreased33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products63.00%HighRestored33929512
Zea mays NA Chloroplast rpl20 308 CDS C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products63.00%HighNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products29.00%LowDecreased33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products64.00%HighRestored33929512
Zea mays NA Chloroplast rpoC2 2774 CDS C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products68.00%HighNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products48.00%MediumDecreased33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products80.00%HighRestored33929512
Zea mays NA Chloroplast rps8 182 CDS C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22Wild TypeControlControlNo mutantNormalNA10 days after germinationDirect sequencing of the RT–PCR products88.00%HighNone33929512
W22zmppr26Mu insertionA Mu8 transposable element was inserted at 683 nt downstream of the ZmPPR26 translation start codon. The maize zmppr26-683 mutant was isolated from the UniformMu population which was generated by intrHeterozygousThe zmppr26 mutant exhibited normal kernel development, as indicated by the ear of a self-pollinated heterozygote, but roughly a quarter of the kernels from the ear showed an albino seedling phenotype after germination. These seedlings died about three weeks later. Linkage analysis in an F2 population of selfed heterozygous ZmPPR26/- showed that the albino seedling-lethal phenotype is tightly linked to the Mu8 insertion in ZmPPR26. No wild type ZmPPR26 mRNA could be detected in the albino leaves of mutant by quantitative real-time PCR (qRT-PCR) analysis, suggesting that this zmppr26 mutation is probably null. Chlorophyll content was much lower in the zmppr26 mutant leaves than in the wild typeNA10 days after germinationDirect sequencing of the RT–PCR products68.00%HighDecreased33929512
KN5585zmppr26/pUbi::ZmPPR26ComplementationThe full-length ZmPPR26 coding sequence with the stop codon was amplified by PCR with primers ZmPPR26FL-F and ZmPPR26FL-R from genomic DNA of leaves . The PCR product was digested by BamHI and XhoI, aHomozygousqRT–PCR analysis in seedling leaves showed that ZmPPR26 was expressed 10-400 times higher in these transgenic lines than in wild type. Genotyping of these seedlings identified homozygous zmppr26 mutants carrying the transgene that had fully green leavesNA10 days after germinationDirect sequencing of the RT–PCR products92.00%HighRestored33929512
Last update: Jul 2021 (version 1.0)