| Project ID: | GBM-011 |
| Sample ID: | GBM-011-01-1B |
| Sample type: | Tumour |
| Submitted by: | NCBI (GEO) |
| Country: | USA |
| BioProject | PRJNA330719 |
| Sample name | BT_S1 |
| SRA accession: | SRP079058 |
| SRS accession: | NA |
| SRX accession: | SRX1964810 - SRX1965298 |
| SRR accession: | SRR3935518 - SRR3936006 |
| GEO accession: | GSE84465 |
| Cancer type: | Glioblastoma |
| Cancer type abbreviation: | GBM |
| Primary site: | Brain |
| Sampling site: | Brain |
| Tumor grade: | IV |
| Tumor status: | Primary |
| Donor age: | 55 |
| Donor gender: | NA |
| Treatment: | NA |
| Other metadata: | Tumor core as well as surrounding peripheral tissue |
| Project title: | Single-Cell RNAseq analysis of diffuse neoplastic infiltrating cells at the migrating front of human glioblastoma |
| Project abstract: | We used single-cell RNAseq to investigate the heterogeneity of glioblastoma tumors and assess differential expression between cells within and in proximity of the tumor. Overall design: Examination of cell types in human primary glioblastoma samples. |
| Construction protocol: | Single-cell sorting in 96-well plates followed by cDNA preparation using Smart-seq2 Smart-Seq2 for preparation of cDNA followed by library preparation using Nextera XT. Nextera tagmentation according to the standard protocol for single cell RNA-seq following Smart-Seq2. |
| Protocol: | Smart-seq2 |
| Instrument: | Illumina NextSeq 500 |
| Strategy: | RNA-Seq |
| Layout: | PAIRED |
| Publications: |
S. Darmanis et al.,
Single-Cell RNA-Seq Analysis of Infiltrating Neoplastic Cells at the Migrating Front of Human Glioblastoma. Cell Reports, 2017, 21(5): 1399-1410
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