Project ID: | GBM-011 |
Sample ID: | GBM-011-01-1B |
Sample type: | Tumour |
Submitted by: | NCBI (GEO) |
Country: | USA |
BioProject | PRJNA330719 |
Sample name | BT_S1 |
SRA accession: | SRP079058 |
SRS accession: | NA |
SRX accession: | SRX1964810 - SRX1965298 |
SRR accession: | SRR3935518 - SRR3936006 |
GEO accession: | GSE84465 |
Cancer type: | Glioblastoma |
Cancer type abbreviation: | GBM |
Primary site: | Brain |
Sampling site: | Brain |
Tumor grade: | IV |
Tumor status: | Primary |
Donor age: | 55 |
Donor gender: | NA |
Treatment: | NA |
Other metadata: | Tumor core as well as surrounding peripheral tissue |
Project title: | Single-Cell RNAseq analysis of diffuse neoplastic infiltrating cells at the migrating front of human glioblastoma |
Project abstract: | We used single-cell RNAseq to investigate the heterogeneity of glioblastoma tumors and assess differential expression between cells within and in proximity of the tumor. Overall design: Examination of cell types in human primary glioblastoma samples. |
Construction protocol: | Single-cell sorting in 96-well plates followed by cDNA preparation using Smart-seq2 Smart-Seq2 for preparation of cDNA followed by library preparation using Nextera XT. Nextera tagmentation according to the standard protocol for single cell RNA-seq following Smart-Seq2. |
Protocol: | Smart-seq2 |
Instrument: | Illumina NextSeq 500 |
Strategy: | RNA-Seq |
Layout: | PAIRED |
Publications: |
S. Darmanis et al.,
Single-Cell RNA-Seq Analysis of Infiltrating Neoplastic Cells at the Migrating Front of Human Glioblastoma. Cell Reports, 2017, 21(5): 1399-1410
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