Data Statistics and tSNE/UMAP Visualization

  • Overview of the dataset
  • tSNE/UMAP - unsupervised clustering
  • The dataset included a total of 272 cells, the parameters adopted were as follows: resolution=1, PCs=1:20, MT<10%.

Tumor Microenvironment

  • Cell components
  • Subtype Cell Count Percentage
    Monocytes 12 4.410%
    Macrophages 119 43.750%
    Fibroblasts 10 3.680%
    Dendritic cells 12 4.410%
    Malignant cells 111 40.810%
    Unassigned 8 2.940%
  • Differential expression analysis
  • Loading, please wait
    Gene Symbol
    Avg_logFC
    Pct.1
    Pct.2
    p-value
    adj_pvalue
    ACY30.310.3330.0087.034e-141.776e-09
    PLEK1.981.0000.4421.462e-083.691e-04
    STX111.360.9170.3231.977e-074.991e-03
    CCL3L31.741.0000.3883.878e-079.790e-03
    CCL4L21.761.0000.4233.960e-079.998e-03
    LPL1.170.8330.2384.863e-071.228e-02
    ICAM11.090.8330.2354.964e-071.253e-02
    CCL41.621.0000.4465.360e-071.353e-02
    GLUD1P30.630.6670.1655.657e-071.428e-02
    TNF0.540.9170.2386.580e-071.661e-02
    Showing 1 to 10 of 285 rows rows per page
  • GO and KEGG enrichments

Functional Genes' Expression

  • Receptor and ligand genes
  • Receptor genes and their paired ligand genes were collected from CelltalkDB, SingleCellSingalR, Cellinker, Cell-Cell Interaction Database and Armingol E, et al., 2021. The following figures only demonstrated the expression of receptor genes and ligand genes supported by three or more databases or other sources, and the corresponding relationship between ligands and receptors were shown in the following table.

  • Oncogenes and suppressor genes
  • Oncogenes and tumor suppressor genes (TSGs) were collected from Cancer Gene Census (CGC), OncoKB, Network of Cancer Genes (NCG), TSGene, IntOGene and Volkan Okur and Wendy K. Chung, 2017. The following figures only displayed the expression of oncogenes and TSGs supported by three or more databases or other sources.