Diversity of wheat anti-microbial peptides.

Tsezi A Egorov, Tatyana I Odintsova, Vitaliy A Pukhalsky, Eugene V Grishin
Author Information
  1. Tsezi A Egorov: Group of Antimicrobial Peptides, Laboratory of Neuroreceptors and Neuroregulators, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation. ego@ibch.ru

Abstract

From seeds of Triticum kiharae Dorof. et Migusch., 24 novel anti-microbial peptides were isolated and characterized by a combination of three-step HPLC (affinity, size-exclusion and reversed-phase) with matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and Edman degradation. Based on sequence similarity and cysteine motifs, partially sequenced peptides were assigned to 7 families: defensins, thionins, lipid-transfer proteins, hevein-like peptides, knottin-like peptides, glycine-rich peptides, and MBP-1 homologs. A novel subfamily of defensins consisting of 6 peptides and a new family of glycine-rich (8 peptides with different repeat motifs) were identified. Three 6-cysteine knottin-like peptides represented by N- and C-terminally truncated variants revealed no sequence homology to any known plant anti-microbial peptides. A new 8-cysteine hevein-like peptide and three 4-cysteine peptides homologous to MBP-1 from maize were isolated. This is the first communication on the occurrence of nearly all families of plant anti-microbial peptides in a single species.

MeSH Term

Anti-Infective Agents
Chromatography, High Pressure Liquid
Peptides
Plant Proteins
Seeds
Sequence Analysis, Protein
Sequence Homology, Amino Acid
Triticum

Chemicals

Anti-Infective Agents
Peptides
Plant Proteins

Word Cloud

Created with Highcharts 10.0.0peptidesanti-microbialnovelisolatedsequencemotifsdefensinshevein-likeknottin-likeglycine-richMBP-1newplantseedsTriticumkiharaeDorofetMigusch24characterizedcombinationthree-stepHPLCaffinitysize-exclusionreversed-phasematrix-assistedlaser-desorption/ionizationtime-of-flightMALDI-TOFmassspectrometryEdmandegradationBasedsimilaritycysteinepartiallysequencedassigned7families:thioninslipid-transferproteinshomologssubfamilyconsisting6family8differentrepeatidentifiedThree6-cysteinerepresentedN-C-terminallytruncatedvariantsrevealedhomologyknown8-cysteinepeptidethree4-cysteinehomologousmaizefirstcommunicationoccurrencenearlyfamiliessinglespeciesDiversitywheat

Similar Articles

Cited By (37)