DYW2 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_Os03g55840

Chloroplast

atpA

1148

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

matK

1258

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	72.00%	High	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhA

1070

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhA

473

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhA

563

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

1481

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

467

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

586

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

611

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

704

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

737

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	58.00%	Medium	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

830

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	71.00%	High	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	45.00%	Medium	Decreased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	Similar	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhD

878

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	88.00%	High	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	31.00%	Low	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	26.00%	Low	Decreased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	89.00%	High	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	95.00%	High	Similar	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhG

5'UTR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	24.00%	Low	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	18.00%	Poor	Decreased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ndhG

347

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	69.00%	High	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	66.00%	High	Decreased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rpl2

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	77.00%	High	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	28.00%	Low	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	36.00%	Low	Decreased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rpoB

467

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	39.00%	Low	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	38.00%	Low	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	54.00%	Medium	Increased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rpoB

545

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	61.00%	High	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	84.00%	High	Increased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	74.00%	High	Increased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rpoB

560

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	72.00%	High	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	79.00%	High	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	86.00%	High	Increased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rpoC2

4106

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	91.00%	High	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rps14

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	49.00%	Medium	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	0.00%	Unedited	Absent	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rps2

134

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	80.00%	High	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	81.00%	High	Decreased	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

rps8

182

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	95.00%	High	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	85.00%	High	Similar	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	88.00%	High	Similar	41246891

Oryza sativa

LOC_Os03g55840

Chloroplast

ycf3

185

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	ZH11 (35 degrees Celsius)	Grown under high-temperature conditions	Grown under high-temperature conditions (35 degrees Celsius)	No mutant	Normal	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	100.00%	Complete	None	41246891
NA	osdyw2-1 (35 degrees Celsius)	A single thymine insertion	A single thymine insertion at the guide RNA target site within the LOC_Os03g55840 gene	Homozygous	Pale-yellow leaves, displaying a high-temperature-sensitive albino lethal phenotype	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	84.00%	High	Decreased	41246891
NA	osdyw2-2 (35 degrees Celsius)	A single cytosine deletion	A single cytosine deletion, which also causes a frameshift and premature translation termination	Homozygous	Albino lethal seedlings phenotype with pale-yellow leaves and reduced Chl contents	Leaf	Third-leaf-stage	RT-PCR products were directly sequenced	80.00%	High	Decreased	41246891

Editing Factor:	DYW2
Synonym:	DYW domain protein 2; BLX
Description:	NUWA and DYW2 Are Dually Targeted to Chloroplast and Mitochondria; NUWA and DYW2 Proteins Are Required for Embryo Development; DYW2 and NUWA Proteins Are Functionally Linked and Involved in Editing of Chloroplast and Mitochondria Transcripts; DYW2 and NUWA Are Required for Editing by PPR-E+
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	OsMORF2b; OsMORF8a; OsMORF8b; OsMORF9
Construct Structure:	DYW
Gene ID & Species:	LOC_Os03g55840 (Oryza sativa)
Edited Gene(s):	matK ndhA ndhB ndhD ndhG rpl2 rpoC2 rps14 atpA ndhF rpoB rps2 rps8 ycf3
Editing Type(s):	C-to-U (78)
Publication(s):	[1] A temperature-sensitive complex formed by OsDYW2 is required for chloroplast RNA editing and splicing under high temperature in rice, New Phytologist, 2025. [doi:10.1111/nph.70744]

Summary

Editing Details