PGL2 - Plant Editosome Database

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	86.50%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	90.61%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	72.21%	High	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	74.35%	High	Decreased	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	92.33%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	80.00%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	68.57%	High	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	78.67%	High	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	77.03%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	87.68%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	53.58%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	59.70%	Medium	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	61.89%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	84.78%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	33.91%	Low	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	51.22%	Medium	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	82.14%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	90.21%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	60.49%	High	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	66.95%	High	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	73.86%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	92.19%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	47.35%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	71.96%	High	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	76.93%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	89.32%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	61.82%	High	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	72.92%	High	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	78.49%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	91.12%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	61.73%	High	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	75.21%	High	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	64.08%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	91.35%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	50.08%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	81.80%	High	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	69.47%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	91.50%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	49.78%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	79.97%	High	Decreased	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	41.35%	Medium	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	83.96%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	18.75%	Poor	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	55.69%	Medium	Decreased	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	44.95%	Medium	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	46.69%	Medium	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	28.91%	Low	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	37.30%	Low	Similar	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	73.33%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	84.51%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	57.33%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	71.82%	High	Decreased	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	64.74%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	80.82%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	42.93%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	52.57%	Medium	Decreased	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	74.02%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	62.27%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	60.08%	High	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	47.24%	Medium	Decreased	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	36.84%	Low	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	0.00%	Unedited	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	0.00%	Unedited	Absent	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	0.00%	Unedited	Similar	34567023
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	80.00%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	71.91%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	51.23%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	49.59%	Medium	Decreased	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	87.54%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	75.43%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	77.29%	High	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	68.19%	High	Similar	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	79.02%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	77.48%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	0.00%	Unedited	Absent	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	0.00%	Unedited	Absent	34567023

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Hedou12 (HD12)	HD12-S1	Control	Control	No mutant	Normal	Leaf	12-day-old	RNA-Seq	84.39%	High	None	34567023
Hedou12 (HD12)	HD12-S2	Control	Control	No mutant	Normal	Seedling	18-day-old	RNA-Seq	72.97%	High	None	34567023
Hedou12 (HD12)	Gmpgl2-S1	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Leaf	12-day-old	RNA-Seq	57.89%	Medium	Decreased	34567023
Hedou12 (HD12)	Gmpgl2-S2	A single-nucleotide deletion	The Gmpgl2 mutant was screened in June 2011 from the 60-CO γ-radiation mutagenized Hedou12 (HD12) population as described previously (Cheng et al., 2016a). Genetic mapping revealed that a single-nucle	NA	Pale green leaf. The components of total chlorophyll (Chl), chlorophyll a (Chla), and chlorophyll b (Chlb) in the leaves of the Gmpgl2 mutants decreased by 40.4, 42.7, and 35.7% of the respective values in the wild-type leaves. The carotenoid (Car) content in the leaves of the mutant plants was 80% that in the leaves of wild-type plants. Furthermore, the ratio of Chl/Car in the Gmpgl2 mutant was lower than in the wild type.	Seedling	18-day-old	RNA-Seq	50.63%	Medium	Decreased	34567023

Editing Factor:	PGL2
Synonym:	Pale Green Leaf 2; GmPGL2
Description:	A Glycine max pale green leaf 2 mutant (Gmpgl2) was identified with decreased chlorophyll contents; The C-to-U editing efficiencies of rps16, rps18, ndhB, ndhD, ndhE, and ndhF were reduced in the Gmpgl2 mutant; RNA electrophoresis mobility shift assay (REMSA) analysis further revealed that GmPGL2 binds to the immediate upstream sequences at RNA editing sites of rps16 and ndhB in vitro, respectively; In addition, GmPGL2 was found to interact with GmMORF8, GmMORF9, and GmORRM6
Protein Family:	PPR
Subclass:	E+
Physical Interaction:	MORF8; MORF9; ORRM6
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	Glyma.05g132700 (Glycine max)
Edited Gene(s):	atpF ndhB ndhD ndhE rpoC1 rps14 rps16 rps18 rps12
Editing Type(s):	C-to-U (80)
Publication(s):	[1] , Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean., Frontiers in plant science, 2021. [PMID=34567023]

Summary

Editing Details