ORRM1 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: ORRM1
Synonym: ORGANELLE RRM PROTEIN 1
Description: Transfection of Arabidopsis orrm1 mutant protoplasts with constructs encoding a region encompassing the RIP-RIP domain or a region spanning the RRM domain of ORRM1 demonstrated that the RRM domain is sufficient for the editing function of ORRM1 in vitro; According to a yeast two-hybrid assay, ORRM1 interacts selectively with pentatricopeptide transfactors via its RIP-RIP domain; Phylogenetic analysis reveals that the RRM in ORRM1 clusters with a clade of RRM proteins that are targeted to organelles; In the orrm1 mutant, 21 plastid RNA editing sites were affected but none are essential to photosynthesis; Morphological and physiological analyses showed that the orrm1 orrm6 double mutants had a smaller plant size, reduced chlorophyll contents, and decreased photosynthetic efficiency, similar to the orrm6 single mutants; Although the orrm1 orrm6 double mutants adopted the phenotype of the orrm6 single mutants, the total number of plastid RNA editing sites affected in the orrm1 orrm6 double mutants was the sum of the sites affected in the orrm1 and orrm6 single mutants
Protein Family: Organelle RNA recognition motif-containing (ORRM)
Subclass: NA
Physical Interaction: RARE1; CRR28; OTP82
Construct Structure: NA
Gene ID & Species: GRMZM5G899787 (Zea mays)
Edited Gene(s): atpA    matK    ndhA    ndhB    ndhD    ndhG    petB    rpl2    rpl20    rpoB    rpoC2    rps14    rps8    ycf3
Editing Type(s): C-to-U (56)
Publication(s): [1] An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize, PNAS, 2013. [doi:10.1073/pnas.1220162110]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays GRMZM5G899787 Chloroplast atpA 1148 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast matK 1258 CDS NA NA NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhA 1070 CDS NA NA NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhA 50 CDS NA NA NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 467 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay31.00%LowDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlNo mutantNormalLeafNAPoisoned Primer Extension (PPE) Assay97.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 586 CDS NA NA NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay7.00%PoorDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlNo mutantNormalLeafNAPoisoned Primer Extension (PPE) Assay98.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 737 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 830 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay65.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlNo mutantNormalLeafNAPoisoned Primer Extension (PPE) Assay99.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhD 878 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay29.00%LowDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlNo mutantNormalLeafNAPoisoned Primer Extension (PPE) Assay96.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhG 347 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast petB 668 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast rpl2 2 CDS NA NA NA NA C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products70.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpl20 308 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoB 467 CDS NA NA NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoB 545 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay14.00%PoorDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
NAWTControlControlNo mutantNormalLeafNAPoisoned Primer Extension (PPE) Assay89.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoB 560 CDS NA NA NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoB 617 CDS NA NA NA NA C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products40.00%MediumDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products80.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoC2 2774 CDS NA NA NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast rps14 80 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay90.00%HighDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlNo mutantNormalLeafNAPoisoned Primer Extension (PPE) Assay97.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rps8 182 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products30.00%LowDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ycf3 185 CDS NA NA NA NA C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ycf3 44 CDS NA NA NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAWTControlControlNo mutantNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Last update: Sep 2026 (version 2.0)