Sample preparation, data collection, and preliminary data analysis in biomolecular solution X-ray scattering.

Alexander Grishaev
Author Information
  1. Alexander Grishaev: Laboratory of Chemical Physics, National Institutes of Health, NIDDK, Bethesda, Maryland.

Abstract

In addition to the classic methods of structural biology--X-ray crystallography and NMR--solution X-ray scattering (SAXS) is playing an increasingly important role in structural investigation of biological macromolecules. However, the simultaneous ease of SAXS data collection and sophistication of its data analysis tools can present challenges to the investigator. Any sample, whether pure or contaminated, whether monodisperse or polydisperse, will yield scattering data, and it is up to the user to ensure the absence of artifacts and to choose a proper structural modeling strategy. This unit discusses experimental aspects of X-ray solution scattering, including sample preparation and data collection, as well as the steps in data processing and preliminary analysis required to ensure the absence of artifacts. The goal is to summarize everything than can go wrong with SAXS data measurement so the user can have confidence in the data before undertaking structural modeling.

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Grants

  1. Z99 DA999999/Intramural NIH HHS
  2. Z99 DK999999/Intramural NIH HHS
  3. Z99 OD999999/Intramural NIH HHS

MeSH Term

Algorithms
Buffers
Data Interpretation, Statistical
Fourier Analysis
Proteins
Scattering, Small Angle
Solutions
X-Ray Diffraction

Chemicals

Buffers
Proteins
Solutions

Word Cloud

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