OZ1 - Plant Editosome Database

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	27.00%	Low	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	95.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	2.00%	Poor	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	77.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	61.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	18.52%	Poor	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	9.77%	Poor	Restored	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	12.94%	Poor	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	7.89%	Poor	Restored	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	11.76%	Poor	Restored	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	11.39%	Poor	Restored	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	13.33%	Poor	Restored	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	8.43%	Poor	Restored	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	31.25%	Low	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	17.65%	Poor	Restored	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	30.49%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	34.52%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	45.68%	Medium	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	11.76%	Poor	Restored	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	21.33%	Low	Restored	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	32.50%	Low	Restored	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	9.30%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	15.50%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	10.45%	Poor	Decreased	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	10.79%	Poor	Decreased	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	17.88%	Poor	Restored	34743362
SAIL_358_H03	Znf-2_64-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	7.87%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	10.15%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	9.41%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-J	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	11.33%	Poor	Decreased	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	12.00%	Poor	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	85.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	61.54%	High	Restored	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	38.10%	Low	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	28.57%	Low	Decreased	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	11.82%	Poor	Decreased	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	15.28%	Poor	Decreased	34743362
SAIL_358_H03	Znf-2_64-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	9.77%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-21	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	6.84%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	5.11%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	21.54%	Low	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	9.42%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-H	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	15.00%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1&2_73-J	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	15.28%	Poor	Decreased	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	99.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	22.06%	Low	Decreased	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	100.00%	Complete	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	80.77%	High	Restored	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	46.34%	Medium	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	83.33%	High	Restored	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	18.31%	Poor	Decreased	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	21.43%	Low	Decreased	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	69.64%	High	Restored	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	24.24%	Low	Decreased	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	13.70%	Poor	Decreased	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	22.39%	Low	Decreased	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	100.00%	Complete	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	25.00%	Low	Decreased	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	45.16%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	77.78%	High	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	78.43%	High	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	17.91%	Poor	Decreased	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	10.45%	Poor	Decreased	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	17.46%	Poor	Decreased	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	85.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	90.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	80.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	95.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	84.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	100.00%	Complete	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	65.06%	High	Restored	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	52.94%	Medium	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	66.67%	High	Restored	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	7.94%	Poor	Decreased	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	9.52%	Poor	Decreased	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	13.64%	Poor	Restored	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	17.98%	Poor	Restored	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	14.44%	Poor	Restored	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	100.00%	Complete	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	29.35%	Low	Restored	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	50.00%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	77.59%	High	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	80.25%	High	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	6.25%	Poor	Decreased	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	19.32%	Poor	Restored	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	73.53%	High	Restored	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	47.78%	Medium	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	27.18%	Low	Restored	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	17.53%	Poor	Restored	34743362
SAIL_358_H03	Znf-1&2_73-21	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-H	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-J	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	5.00%	Poor	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	92.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	2.00%	Poor	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	22.00%	Low	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	68.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	98.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	7.00%	Poor	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	97.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	95.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	13.79%	Poor	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	11.86%	Poor	Restored	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	11.86%	Poor	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	8.77%	Poor	Restored	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	8.77%	Poor	Restored	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	5.08%	Poor	Restored	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	9.62%	Poor	Restored	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	40.32%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	20.75%	Low	Restored	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	41.67%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	37.29%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	59.68%	Medium	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	9.10%	Poor	Restored	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	26.21%	Low	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	11.27%	Poor	Restored	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-21	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-H	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-J	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	90.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	44.44%	Medium	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	28.26%	Low	Restored	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	13.04%	Poor	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	21.28%	Low	Restored	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	76.19%	High	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	9.43%	Poor	Decreased	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	32.73%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	44.23%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	57.69%	Medium	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	22.67%	Low	Restored	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	23.38%	Low	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	5.95%	Poor	Decreased	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-21	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-H	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-J	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	56.00%	Medium	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	16.00%	Poor	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	91.00%	High	None	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	91.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	9.43%	Poor	Decreased	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	48.21%	Medium	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	46.94%	Medium	Restored	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	45.10%	Medium	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	42.86%	Medium	Restored	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	8.77%	Poor	Decreased	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	8.62%	Poor	Decreased	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	22.81%	Low	Restored	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	33.33%	Low	Restored	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	26.79%	Low	Restored	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	88.64%	High	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	25.00%	Low	Restored	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	41.82%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	61.82%	High	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	67.27%	High	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	56.25%	Medium	Restored	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	43.96%	Medium	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	27.84%	Low	Restored	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-21	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-H	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	5.00%	Poor	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	85.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	10.00%	Poor	Decreased	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	52.00%	Medium	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	39.13%	Low	Restored	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	34.69%	Low	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	33.33%	Low	Restored	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	9.62%	Poor	Decreased	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	10.00%	Poor	Decreased	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	12.24%	Poor	Decreased	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	20.00%	Low	Restored	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	12.00%	Poor	Decreased	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	85.11%	High	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	18.37%	Poor	Decreased	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	34.69%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	48.98%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	51.02%	Medium	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	6.12%	Poor	Decreased	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	6.12%	Poor	Decreased	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	53.13%	Medium	Restored	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	36.36%	Low	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	23.08%	Low	Restored	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-21	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-H	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	33.00%	Low	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	83.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	80.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	97.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	84.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	80.70%	High	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	11.11%	Poor	Restored	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	17.78%	Poor	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	21.57%	Low	Restored	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	47.27%	Medium	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	29.41%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	45.28%	Medium	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	17.53%	Poor	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	13.40%	Poor	Restored	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-J	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	45.00%	Medium	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	78.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	88.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	97.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	87.00%	High	Increased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	92.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	67.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	83.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	71.00%	High	None	25768119
SAIL_358_H03	oz1 -/-	T-DNA insertion	oz1 T-DNA insertional mutants (SAIL_358_H03) were purchased from the Arabidopsis Biological Resource Center (https://abrc.osu.edu)	Homozygous; Knockout	oz1 knockout mutants exhibit reduced seed viability and early chlorosis	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
Columbia (Col-3)	WT	Control	Control	No mutant	Normal	Leaf	1-5-month-old	Sanger sequencing	84.42%	High	None	34743362
SAIL_358_H03	RecA-OZ1_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	RecA-OZ1_B	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	RecA-OZ1_C	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	Seedlings and mature plants expressing the full-length OZ1 construct resembled wild-type Arabidopsis, but plants expressing truncation constructs exhibited varying degrees of chlorosis and delayed growth	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	N-terminus	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminal part of OZ1 under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	N+Znf	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the N-terminus+Znf under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amin	Homozygous; Knockout	The plants expressing the N-terminal part of OZ1 or the N-terminus+Znf showed severe chlorosis similar to the oz1 mutant plant.	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_C-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf_E-47	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	9.57%	Poor	Decreased	34743362
SAIL_358_H03	Znf_E-100	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the Znf alone under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the first 65 amino aci	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Decreased	34743362
SAIL_358_H03	Znf+C_G	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	11.29%	Poor	Restored	34743362
SAIL_358_H03	Znf+C_C-8	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	7.69%	Poor	Decreased	34743362
SAIL_358_H03	Znf+C_C-21	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	23.08%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-57	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	22.86%	Low	Restored	34743362
SAIL_358_H03	Znf+C_D-84	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	42.86%	Medium	Restored	34743362
SAIL_358_H03	C-terminus_A	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	C-terminus_B-98	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	24.19%	Low	Restored	34743362
SAIL_358_H03	C-terminus_B-62	Plant transformation	Producing stable transformants of oz1 homozygous plants expressing the full-length OZ1 construct under control of the 35S promoter and targeted to the chloroplast with the RecA transit peptide (the fi	Homozygous; Knockout	The transgenic plants expressing constructs with the Znf alone, Znf+C-terminus or the C-terminus alone exhibited some greening	Leaf	1-5-month-old	Sanger sequencing	15.38%	Poor	Restored	34743362
SAIL_358_H03	Znf-1_67-1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1_67-B-64	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	10.26%	Poor	Restored	34743362
SAIL_358_H03	Znf-1_67-D-29	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf1	Homozygous; Knockout	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64_1	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64_3	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-2_64-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-21	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-A	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-B	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-D	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-H	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362
SAIL_358_H03	Znf-1&2_73-J	Plant transformation; Mutation	oz1 homozygous background plants expressing OZ1 constructs with the cystine-to-alanine mutations in both Znf1 and Znf2	Knockout; Heterozygous	Yellow leaves at 3 weeks, green leaves at 2.5 months	Leaf	1-5-month-old	Sanger sequencing	0.00%	Unedited	Absent	34743362

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	44.00%	Medium	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	82.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	76.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	91.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	60.00%	High	Decreased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	92.00%	High	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	72.00%	High	Increased	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	24.00%	Low	None	25768119

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_358_H03	oz1-1	T-DNA Insertion	NA	Homozygous	Uniform yellow phenotype as a young seedling; Subsequent growth on sucrose media result in the appearance of light green; Non-variegated leaves	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	25768119
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	28.00%	Low	None	25768119

Editing Factor:	OZ1
Synonym:	ORGANELLE ZINC FINGER 1, VAR3, VARIEGATED 3
Description:	Encoding a zinc finger motif-containing protein essential for chloroplast RNA editing; Mutation of key structural residues in the Znf domains showed that they are necessary for editing and required for interaction with ORRM1, a general editing factor with an RNA-binding domain
Protein Family:	OZ
Subclass:	NA
Construct Structure:	NA, N-terminal motif-Znf-Znf-C-ter
Gene ID & Species:	AT5G17790 (Arabidopsis thaliana)
Edited Gene(s):	ndhD petL ndhG psbZ rps12 psbF rpl23 ndhB rpoA ndhF rpoB rpoC1 clpP matK accD
Editing Type(s):	C-to-U (329)
Publication(s):	[1] The RanBP2 zinc finger domains of chloroplast RNA editing factor OZ1 are required for protein-protein interactions and conversion of C to U., The Plant journal : for cell and molecular biology, 2022. [PMID=34743362] [2] A zinc finger motif-containing protein is essential for chloroplast RNA editing., PLoS genetics, 2015. [PMID=25768119]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details