PPGR - Gene Detail

Basic Information

Gene ID	Juni_Chr2.287.g View in Genome Browser
Position	Chr2:3131548-3131757 (+) 209bp
Gene Type	gene
Gene Description (Protein Product)	"Catalytic subunit of the molybdopterin synthase complex
Organism	Juglans nigra
Also AS	AT2G43760

upstream:

Domain

Database	EntryID	E-Value	Start	end	InterPro ID	Description

Regulation&Interaction

Protein-protein interaction (PPI)

Juni_Chr4.889.g Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin

Juni_Chr7.3118.g Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates

Juni_Chr9.1650.g Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

Regulatory gene

Juni_Chr1.180.g Agamous-like MADS-box protein AGL12

Juni_Chr1.186.g Agamous-like MADS-box protein

Juni_Chr1.243.g Cyclic dof factor

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Annotation

Orthologous Group

Orthologous ID	Species Number	All hits in PereRegDB	Hits of this species	Orthologous Detail

Expression Profile

DataSet	Number of Samples expressed(TPM>1)	Mean	Min	Max	Standard deviation(SD)	Coeffcient variation(CV)

Select Expression

Pathway

GO Term	Description	GO Category
GO:0003674	molecular_function	MF
GO:0003824	catalytic activity	MF
GO:0005575	cellular_component	CC
GO:0005622	intracellular anatomical structure	CC
GO:0005623	obsolete cell	CC
GO:0005737	cytoplasm	CC
GO:0005829	cytosol	CC
GO:0006139	nucleobase-containing compound metabolic process	BP
GO:0006163	purine nucleotide metabolic process	BP
GO:0006725	cellular aromatic compound metabolic process	BP
GO:0006732	obsolete coenzyme metabolic process	BP
GO:0006753	nucleoside phosphate metabolic process	BP
GO:0006793	phosphorus metabolic process	BP
GO:0006796	phosphate-containing compound metabolic process	BP
GO:0006807	nitrogen compound metabolic process	BP
GO:0008150	biological_process	BP
GO:0008152	metabolic process	BP
GO:0009058	biosynthetic process	BP
GO:0009116	nucleoside metabolic process	BP
GO:0009117	nucleotide metabolic process	BP
GO:0009119	ribonucleoside metabolic process	BP
GO:0009141	nucleoside triphosphate metabolic process	BP
GO:0009144	purine nucleoside triphosphate metabolic process	BP
GO:0009150	purine ribonucleotide metabolic process	BP
GO:0009199	ribonucleoside triphosphate metabolic process	BP
GO:0009205	purine ribonucleoside triphosphate metabolic process	BP
GO:0009259	ribonucleotide metabolic process	BP
GO:0009987	cellular process	BP
GO:0016740	transferase activity	MF
GO:0016782	transferase activity, transferring sulphur-containing groups	MF
GO:0016783	sulfurtransferase activity	MF
GO:0018130	heterocycle biosynthetic process	BP
GO:0019538	protein metabolic process	BP
GO:0019637	organophosphate metabolic process	BP
GO:0019693	ribose phosphate metabolic process	BP
GO:0030366	molybdopterin synthase activity	MF
GO:0032324	molybdopterin cofactor biosynthetic process	BP
GO:0034641	cellular nitrogen compound metabolic process	BP
GO:0042278	purine nucleoside metabolic process	BP
GO:0043170	macromolecule metabolic process	BP
GO:0043545	molybdopterin cofactor metabolic process	BP
GO:0044237	cellular metabolic process	BP
GO:0044238	primary metabolic process	BP
GO:0044249	cellular biosynthetic process	BP
GO:0044260	cellular macromolecule metabolic process	BP
GO:0044267	protein metabolic process	BP
GO:0044281	small molecule metabolic process	BP
GO:0044424	obsolete intracellular part	CC
GO:0044444	obsolete cytoplasmic part	CC
GO:0044464	obsolete cell part	CC
GO:0046039	GTP metabolic process	BP
GO:0046128	purine ribonucleoside metabolic process	BP
GO:0046483	heterocycle metabolic process	BP
GO:0051186	obsolete cofactor metabolic process	BP
GO:0051188	obsolete cofactor biosynthetic process	BP
GO:0051189	prosthetic group metabolic process	BP
GO:0055086	nucleobase-containing small molecule metabolic process	BP
GO:0071704	organic substance metabolic process	BP
GO:0072521	purine-containing compound metabolic process	BP
GO:0090407	organophosphate biosynthetic process	BP
GO:1901068	guanosine-containing compound metabolic process	BP
GO:1901135	carbohydrate derivative metabolic process	BP
GO:1901360	organic cyclic compound metabolic process	BP
GO:1901362	organic cyclic compound biosynthetic process	BP
GO:1901564	organonitrogen compound metabolic process	BP
GO:1901566	organonitrogen compound biosynthetic process	BP
GO:1901576	organic substance biosynthetic process	BP
GO:1901657	glycosyl compound metabolic process	BP

KEGG Term	Name	Description
map04122	Sulfur relay system	Ubiquitin and ubiquitin-like proteins (Ubls) are signalling messengers that control many cellular functions, such as cell proliferation, apoptosis, and DNA repair. It is suggested that Ub-protein modification evolved from prokaryotic sulfurtransfer systems. Molybdenum cofactor (Moco) and thiamin are sulfur-containing cofactors whose biosynthesis includes a key sulfur transfer step that uses unique sulfur carrier proteins, MoaD and ThiS. Ubiquitin, MoaD, and ThiS are all structurally related proteins whose C-termini are activated through adenylation by homologous E1-like enzymes. s2T biosynthesis may share similar chemistry with Moco and thiamin synthesis. In Saccharomyces cerevisiae, Urm1 and Uba4 function as part of a ubl protein conjugation system, though they have sequence homology to bacterial sulfur-transfer enzymes and the ability to function in sulfur transfer.
map01100	Metabolic pathways	-
map00790	Folate biosynthesis	-