PPGR - Gene Detail

Basic Information

Gene ID	JsiChr08G11992.g View in Genome Browser
Position	chr8:25364302-25368687 (-) 4385bp
Gene Type	gene
Gene Description (Protein Product)	"Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys)
Organism	Juglans sigillata
Also AS	AT4G35910

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Domain

Database	EntryID	E-Value	Start	end	InterPro ID	Description

Regulation&Interaction

Protein-protein interaction (PPI)

JsiChr12G11862.g Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor

JsiChr09G10633.g Monothiol

JsiChr09G10055.g permease

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Annotation

Orthologous Group

Orthologous ID	Species Number	All hits in PereRegDB	Hits of this species	Orthologous Detail

Pathway

GO Term	Description	GO Category
GO:0002097	tRNA wobble base modification	BP
GO:0002098	tRNA wobble uridine modification	BP
GO:0002143	tRNA wobble position uridine thiolation	BP
GO:0003674	molecular_function	MF
GO:0003824	catalytic activity	MF
GO:0005575	cellular_component	CC
GO:0005622	intracellular anatomical structure	CC
GO:0005623	obsolete cell	CC
GO:0005737	cytoplasm	CC
GO:0005829	cytosol	CC
GO:0006139	nucleobase-containing compound metabolic process	BP
GO:0006396	RNA processing	BP
GO:0006399	tRNA metabolic process	BP
GO:0006400	tRNA modification	BP
GO:0006725	cellular aromatic compound metabolic process	BP
GO:0006807	nitrogen compound metabolic process	BP
GO:0008033	tRNA processing	BP
GO:0008150	biological_process	BP
GO:0008152	metabolic process	BP
GO:0009451	RNA modification	BP
GO:0009987	cellular process	BP
GO:0010467	gene expression	BP
GO:0016070	RNA metabolic process	BP
GO:0016740	transferase activity	MF
GO:0016782	transferase activity, transferring sulphur-containing groups	MF
GO:0016783	sulfurtransferase activity	MF
GO:0034227	tRNA thio-modification	BP
GO:0034470	ncRNA processing	BP
GO:0034641	cellular nitrogen compound metabolic process	BP
GO:0034660	ncRNA metabolic process	BP
GO:0043170	macromolecule metabolic process	BP
GO:0043412	macromolecule modification	BP
GO:0044237	cellular metabolic process	BP
GO:0044238	primary metabolic process	BP
GO:0044424	obsolete intracellular part	CC
GO:0044444	obsolete cytoplasmic part	CC
GO:0044464	obsolete cell part	CC
GO:0046483	heterocycle metabolic process	BP
GO:0071704	organic substance metabolic process	BP
GO:0090304	nucleic acid metabolic process	BP
GO:1901360	organic cyclic compound metabolic process	BP

KEGG Term	Name	Description
map04122	Sulfur relay system	Ubiquitin and ubiquitin-like proteins (Ubls) are signalling messengers that control many cellular functions, such as cell proliferation, apoptosis, and DNA repair. It is suggested that Ub-protein modification evolved from prokaryotic sulfurtransfer systems. Molybdenum cofactor (Moco) and thiamin are sulfur-containing cofactors whose biosynthesis includes a key sulfur transfer step that uses unique sulfur carrier proteins, MoaD and ThiS. Ubiquitin, MoaD, and ThiS are all structurally related proteins whose C-termini are activated through adenylation by homologous E1-like enzymes. s2T biosynthesis may share similar chemistry with Moco and thiamin synthesis. In Saccharomyces cerevisiae, Urm1 and Uba4 function as part of a ubl protein conjugation system, though they have sequence homology to bacterial sulfur-transfer enzymes and the ability to function in sulfur transfer.