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Plant biotechnology journal
02, 2019;17 (2): 451-460.

Genome sequence and genetic transformation of a widely distributed and cultivated poplar.

Jianchao Ma, Dongshi Wan, Bingbing Duan, Xiaotao Bai, Qiuxian Bai, Ningning Chen, Tao Ma
Author Information
  1. Jianchao Ma: State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China.
  2. Dongshi Wan: State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China.
  3. Bingbing Duan: State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China.
  4. Xiaotao Bai: State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China.
  5. Qiuxian Bai: State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China.
  6. Ningning Chen: State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China.
  7. Tao Ma: State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China. ORCID
PMID: 30044051 DOI: 10.1111/pbi.12989

Abstract

Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by experiments. Through a process of hybrid genome assembly, a total of 464 M of the genome was assembled. Annotation analyses predicted 37 901 protein-coding genes. This genome is highly collinear to that of P. trichocarpa, with most genes having orthologs in the two species. We found a marked expansion of gene families related to histone and the hormone auxin but loss of disease resistance genes in P. alba if compared with the closely related P. trichocarpa. The genome sequence presented here represents a valuable resource for further molecular functional analyses of this species as a new tree model, poplar breeding practices and comparative genomic analyses across different poplars.

Keywords

Populus alba Populus bolleana comparative genomics gene families transformation efficiency

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Grants

  1. 2013AA102605/National High-Tech Research and Development Program of China
  2. 31561123001/National Science Foundation of China
  3. 31470620/National Science Foundation of China

MeSH Term

Genome, Plant
Genomics
Molecular Sequence Annotation
Populus
Sequence Analysis, DNA
Transformation, Genetic
Journal Article Research Support, Non-U.S. Gov't

Links to CNCB-NGDC Resources

GWH: GWHAAEP00000000 (Populus alba var. pyramidalis)
Article has an altmetric score of 1

Word Cloud

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