MEF8 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G25580

Mitochondrion

atp1

1110

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

GUC=>GUU
NA=>NA

V=>V
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	19.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	7.00%	Poor	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	20.04%	Low	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	7.47%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

atp4

382

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CUA=>UUA
NA=>NA

L=>L
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	3.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	44.00%	Medium	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	1.00%	Poor	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	46.56%	Medium	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	3.33%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

atp8

389

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	15.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	4.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmB

338

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	92.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	83.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	93.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmB

367

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	86.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	68.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	84.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmB

379

CDS

C-to-U

CCA/CCA=>UCA/UUA

P=>S/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	85.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	64.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	83.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmB

380

CDS

C-to-U

CCA/CCA=>CUA/UUA

P=>L/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	90.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	79.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	91.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmB

406

CDS

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	47.00%	Medium	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	25.00%	Low	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	39.00%	Low	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmB

576

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	67.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	34.00%	Low	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	45.00%	Medium	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmC

270

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	10.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	17.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	15.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmFc

1150

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CUA=>UUA
NA=>NA

L=>L
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	1.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	7.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	7.74%	Poor	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	0.58%	Poor	Similar	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmFc

1280

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	73.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	58.00%	Medium	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	64.00%	High	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmFc

321

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	7.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	10.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	8.00%	Poor	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmFc

333

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

UUC=>UUU
NA=>NA

F=>F
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	3.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	84.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	3.00%	Poor	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	84.96%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	2.66%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmFc

832

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	2.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	27.00%	Low	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	2.00%	Poor	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	28.93%	Low	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	2.21%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

ccmFn1

378

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	64.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	92.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	65.00%	High	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

cox2

557

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CCU=>CUU
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	52.00%	Medium	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	99.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	35.00%	Low	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.44%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	50.75%	Medium	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

cox3

603

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

UUC=>UUU
NA=>NA

F=>F
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	1.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	14.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	15.08%	Poor	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	0.50%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

cox3

3'UTR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	14.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

matR

1593

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	33.00%	Low	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	55.00%	Medium	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	45.00%	Medium	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

matR

1596

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CCC=>CCU
NA=>NA

P=>P
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	14.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	46.00%	Medium	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	25.00%	Low	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	48.97%	Medium	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	13.75%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

matR

1730

CDS

C-to-U

UCC/UCC=>UUC/UUU

S=>F/F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	72.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	87.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	84.00%	High	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

matR

1731

CDS

C-to-U

UCC/UCC=>UCU/UUU

S=>S/F

Synonymous/Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	43.00%	Medium	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	57.00%	Medium	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	54.00%	Medium	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

matR

1751

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CCU=>CUU
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	50.00%	Medium	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	79.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	67.00%	High	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	79.66%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	49.69%	Medium	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

matR

1771

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	56.00%	Medium	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	75.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	73.00%	High	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

matR

1807

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CAC=>UAC
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	45.00%	Medium	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	73.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	57.00%	Medium	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	72.76%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	44.17%	Medium	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

mttB

407

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CCU/CCU=>CUU/UUU
NA=>NA

P=>L/F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	47.00%	Medium	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	82.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	45.00%	Medium	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	82.08%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	49.92%	Medium	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

mttB

440

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	66.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	80.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	64.00%	High	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

mttB

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	63.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	76.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	70.00%	High	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad2

1233

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	18.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad2

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	74.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	65.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	82.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad2

285

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	10.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	5.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	6.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad3

149

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	89.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	75.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	85.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad3

211

CDS

C-to-U

CCU/CCU=>UCU/UUU

P=>S/F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	90.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	78.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	88.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad3

212

CDS

C-to-U

CCU/CCU=>CUU/UUU

P=>L/F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	90.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	79.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	89.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

1206

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CUC=>CUU
NA=>NA

L=>L
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	6.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	22.00%	Low	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	4.00%	Poor	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	23.79%	Low	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	5.81%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

1401

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	22.00%	Low	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	31.00%	Low	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	22.00%	Low	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

279

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	5.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	2.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	2.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

303

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	10.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	3.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	4.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

385

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	12.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	5.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	7.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

402

CDS

EnsemblGenomes (release 39)

BK010421

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	1.67%	Poor	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	0.40%	Poor	Similar	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

CDS

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	2.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad4

753

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	3.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	4.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad5

1731

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	8.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	17.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad5

676

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CUU=>UUU
NA=>NA

L=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
Col	WT	Control	Control	Control	Normal	Pollen	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
NA	mef8-1	EMS Mutant	Gly498Arg in E+	Homozygous	NA	Leaf	NA	Direct Sequencing of PCR Products	70.00%	High	Decreased	22977245
Salk_106391	mef8-2	T-DNA Insertion	T-DNA interrupts the MEF8 protein within the N-terminal region	Homozygous	NA	Leaf	NA	Direct Sequencing of PCR Products	40.00%	Medium	Decreased	22977245
Salk_106391	mef8-2	T-DNA Insertion	T-DNA interrupts the MEF8 protein within the N-terminal region	Homozygous	NA	Pollen	NA	Direct Sequencing of PCR Products	100.00%	Complete	Similar	22977245
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	57.00%	Medium	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	99.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	44.00%	Medium	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.36%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	57.34%	Medium	Decreased	32098170
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.38%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad5

801

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	26.00%	Low	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	36.00%	Low	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	26.00%	Low	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad5

Intron

NA
EnsemblGenomes (release 39)

NA
BK010421

Genomic position

141966
235403

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	25.00%	Low	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	96.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	19.00%	Poor	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	95.97%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	25.45%	Low	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad6

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
Col	WT	Control	Control	Control	Normal	Pollen	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
NA	mef8-1	EMS Mutant	Gly498Arg in E+	Homozygous	NA	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	22977245
Salk_106391	mef8-2	T-DNA Insertion	T-DNA interrupts the MEF8 protein within the N-terminal region	Homozygous	NA	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	22977245
Salk_106391	mef8-2	T-DNA Insertion	T-DNA interrupts the MEF8 protein within the N-terminal region	Homozygous	NA	Pollen	NA	Direct Sequencing of PCR Products	100.00%	Complete	Similar	22977245
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	79.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	99.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	75.00%	High	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.00%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	77.59%	High	Decreased	32098170
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.01%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	90.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad7

1137

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	88.00%	High	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	64.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	80.00%	High	Increased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad7

283

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

CCA=>UCA
NA=>NA

P=>S
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	3.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	0.00%	Unedited	Absent	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	3.03%	Poor	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	0.03%	Poor	Similar	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad7

910

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	22.00%	Low	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	7.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	12.00%	Poor	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad7

Intron

Genomic position

134282

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	19.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	7.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	4.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

nad9

517

CDS

C-to-U

CAA=>UAA

Q=>Stop codon

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	10.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	4.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	3.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

pseudo-rps14

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	15.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	21.00%	Low	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	17.00%	Poor	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

rpl16

426

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	5.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	5.00%	Poor	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

rpl16

3'UTR

391
72
105

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	61.00%	High	Increased	29035004
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	15.00%	Poor	Increased	29035004
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	17.00%	Poor	Increased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	31.00%	Low	None	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	6.00%	Poor	None	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	9.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	33.00%	Low	Restored	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	6.00%	Poor	Restored	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	8.00%	Poor	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

rpl5

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	76.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	85.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	87.00%	High	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

rpl5

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	76.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	88.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	85.00%	High	Restored	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

rpl5

CDS

C-to-U

CCG/CCG=>CUG/UUG

P=>L/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	77.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	88.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	84.00%	High	Decreased	29035004

Arabidopsis thaliana

AT2G25580

Mitochondrion

rps12

159

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

GCC=>GCU
NA=>NA

A=>A
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	2.00%	Poor	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	14.00%	Poor	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	2.00%	Poor	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	16.53%	Poor	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	2.02%	Poor	Decreased	32098170

Arabidopsis thaliana

AT2G25580

Mitochondrion

rps3

887

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

UCG=>UUG
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK-106391C	mef8	T-DNA Insertion	T-DNA is inserted located upstream of the PPRs	Null allele	NA	Leaf	4-5 weeks	STS-PCRseq	80.00%	High	Decreased	29035004
Col-0	WT	Control	Control	Control	Normal	Leaf	4-5 weeks	STS-PCRseq	90.00%	High	None	29035004
SALK-106391C	HXA	Complementation	mef8 mutant is transformed with a mutated MEF8 where E in HXE has been substituted by HXA	NA	NA	Leaf	4-5 weeks	STS-PCRseq	86.00%	High	Decreased	29035004
NA	WT	Control	Control	Control	NA	NA	NA	RNA-seq	90.69%	High	None	32098170
NA	mef8	NA	NA	NA	NA	NA	NA	RNA-seq	79.07%	High	Decreased	32098170

Editing Factor:	MEF8
Synonym:	MITOCHONDRIAL EDITING FACTOR 8
Description:	Encoding an RNA editing factor which is responsible for two specific sites in plant mitochondria. Mutants of MEF8 show diminished editing in leaves but not in pollen
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AT2G25580 (Arabidopsis thaliana)
Edited Gene(s):	nad5 nad6 ccmFc cox2 atp4 mttB matR ccmFn1 nad4 cox3 rpl5 rps12 rps3 atp1 nad2 ccmC pseudo-rps14 rpl16 nad7 ccmB nad3 atp8 nad9
Editing Type(s):	C-to-U (242)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] A protein with an unusually short PPR domain, MEF8, affects editing at over 60 Arabidopsis mitochondrial C targets of RNA editing., The Plant journal : for cell and molecular biology, 2017. [PMID=29035004] [3] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [4] Two related RNA-editing proteins target the same sites in mitochondria of Arabidopsis thaliana., The Journal of biological chemistry, 2012. [PMID=22977245]

Summary

Editing Details