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RIP1b - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: RIP1b
Synonym: SlRIP1b
Description: RNA-sequencing revealed that SlRIP1b affects the C-U editing of numerous mitochondrial pre-RNA transcripts and in particular altered RNA editing of various cytochrome c maturation (CCM)-related genes; The slrip1b mutants display increased H2 O2 and aberrant mitochondrial morphologies, which are associated with defects in cytochrome c biosynthesis and assembly of respiratory complex III
Protein Family: Multiple organellar RNA editing factor (MORF)
Subclass: NA
Physical Interaction: SlORRM4
Construct Structure: NA
Gene ID & Species: Solyc12g014230 (Solanum lycopersicum)
Edited Gene(s): atp1    atp4    cox1    nad4    nad6    nad9    rps10    rps13    NA    ccmB    ccmFn    nad7    rpoB    atp6    atp9
Editing Type(s): C-to-U (348)
Publication(s): [1] SlRIP1b is a global organellar RNA editing factor, required for normal fruit development in tomato plants, New Phytol, 2023. [doi:10.1111/nph.18594]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Solanum lycopersicum Solyc12g014230 Mitochondrion atp1 1039 CDS v.SL2.50 NC_035963.1 Genomic region 189707 C-to-U NA=>NA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products56.99%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.85%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products48.85%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp1 1178 CDS v.SL2.50 NC_035963.1 Genomic region 189846 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products54.87%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.85%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.98%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp1 1216 CDS v.SL2.50 NC_035963.1 Genomic region 189884 C-to-U NA=>NA L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.82%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.98%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.81%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp1 1415 CDS v.SL2.50 NC_035963.1 Genomic region 190083 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products54.95%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.94%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.72%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp1 1490 CDS v.SL2.50 NC_035963.1 Genomic region 190158 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products56.30%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.89%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products47.58%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp4 171 CDS v.SL2.50 NC_035963.1 Genomic region 49205 C-to-U NA=>NA F=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products32.28%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products22.75%LowSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products20.04%LowDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp4 306 CDS v.SL2.50 NC_035963.1 Genomic region 49070 C-to-U NA=>NA T=>T Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products20.15%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products31.17%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products28.66%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp6 1300 CDS v.SL2.50 NC_035963.1 Genomic region 426432 C-to-U NA=>NA Q=>Stop codon Nonsense
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products71.96%HighNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products82.27%HighIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products82.46%HighIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp9 191 CDS v.SL2.50 NC_035963.1 Genomic region 409877 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products64.40%HighNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products75.54%HighIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products71.77%HighSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp9 212 CDS v.SL2.50 NC_035963.1 Genomic region 409856 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products62.60%HighNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products73.95%HighIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products70.15%HighSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion atp9 223 CDS v.SL2.50 NC_035963.1 Genomic region 409845 C-to-U NA=>NA Q=>Stop codon Nonsense
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products62.45%HighNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products73.91%HighIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products69.72%HighSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion ccmB 28 CDS v.SL2.50 NC_035963.1 Genomic region 205142 C-to-U NA=>NA H=>Y NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products25.10%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products36.48%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products35.06%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion ccmB 43 CDS v.SL2.50 NC_035963.1 Genomic region 205127 C-to-U NA=>NA P=>S NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products23.60%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products36.24%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products35.06%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion ccmFn 395 CDS v.SL2.50 NC_035963.1 Genomic region 319999 C-to-U NA=>NA S=>L NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products16.42%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products27.82%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products23.14%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion ccmFn 737 CDS v.SL2.50 NC_035963.1 Genomic region 320341 C-to-U NA=>NA S=>L NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products12.57%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products23.11%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products19.48%PoorSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion ccmFn 797 CDS v.SL2.50 NC_035963.1 Genomic region 320401 C-to-U NA=>NA S=>L NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products12.71%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products25.05%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products19.46%PoorSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion ccmFn 809 CDS v.SL2.50 NC_035963.1 Genomic region 320413 C-to-U NA=>NA P=>L NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products12.64%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products24.48%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products19.15%PoorSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion ccmFn 824 CDS v.SL2.50 NC_035963.1 Genomic region 320428 C-to-U NA=>NA S=>L NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products12.93%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products23.89%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products18.77%PoorSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 11 CDS v.SL2.50 NC_035963.1 Genomic region 315398 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products36.98%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.64%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.66%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 1405 CDS v.SL2.50 NC_035963.1 Genomic region 316792 C-to-U NA=>NA R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products37.77%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products48.23%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.59%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 1433 CDS v.SL2.50 NC_035963.1 Genomic region 316820 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products37.72%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products48.13%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.30%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 1489 CDS v.SL2.50 NC_035963.1 Genomic region 316876 C-to-U NA=>NA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.17%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.28%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.33%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 1499 CDS v.SL2.50 NC_035963.1 Genomic region 316886 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products53.40%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products42.16%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.75%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 2 CDS v.SL2.50 NC_035963.1 Genomic region 315389 C-to-U NA=>NA T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.60%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.54%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.59%MediumSimilar36345265
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products32.44%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.87%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.45%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 452 CDS v.SL2.50 NC_035963.1 Genomic region 315839 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products36.79%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.75%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.65%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 551 CDS v.SL2.50 NC_035963.1 Genomic region 315938 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products36.00%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.05%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.35%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 590 CDS v.SL2.50 NC_035963.1 Genomic region 315977 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products36.58%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.29%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.30%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 668 CDS v.SL2.50 NC_035963.1 Genomic region 316055 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products36.08%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.78%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.93%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion cox1 746 CDS v.SL2.50 NC_035963.1 Genomic region 316133 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products37.20%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.63%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.95%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 109965 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products15.00%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products19.73%PoorSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products29.51%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 112526 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products45.14%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products31.20%LowDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products31.17%LowDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 148284 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.05%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.89%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.18%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 150866 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products56.56%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.50%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products48.06%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 151006 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products57.75%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.17%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.60%MediumDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 151036 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products57.93%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.74%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products48.02%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 151040 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products57.17%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.74%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.84%MediumDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 152844 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products56.55%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.33%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products47.06%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 195368 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products8.79%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products23.20%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products19.10%PoorIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 195627 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products12.74%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products29.13%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products26.75%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 196184 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products22.03%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products36.62%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products43.57%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 196197 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products27.34%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products39.66%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.61%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 196480 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products29.77%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products38.36%LowSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.86%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 216643 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products7.77%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products19.92%PoorIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products24.21%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 220707 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products54.27%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.86%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.26%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 276254 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products56.60%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.21%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.54%MediumDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 276275 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products56.50%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.36%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.63%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 288342 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products9.71%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products13.94%PoorSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products20.29%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 30204 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products20.18%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products48.23%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products53.07%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 30274 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products22.96%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products49.82%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products57.00%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 30958 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products20.47%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.05%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products53.52%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 334101 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products36.23%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.37%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.61%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 355678 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products32.74%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.68%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products41.04%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 357200 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products33.92%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.61%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products42.24%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 373012 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products22.07%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.51%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products35.56%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 373223 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products43.98%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products53.37%MediumSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products54.66%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 373321 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products26.50%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products38.09%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.23%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 373358 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products45.73%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products56.30%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products54.66%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 374940 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products47.85%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products58.48%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products58.05%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 376398 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products50.95%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products40.22%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products40.05%MediumDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 376407 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products46.46%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products35.66%LowDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products35.31%LowDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 433553 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products8.32%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products8.25%PoorSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products18.57%PoorIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 51124 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products81.41%HighNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products67.93%HighDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products70.96%HighDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 51127 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products81.23%HighNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products67.62%HighDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products70.81%HighDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 51133 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products81.97%HighNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products69.06%HighDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products71.37%HighDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 63971 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products10.60%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products21.93%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products33.07%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 64024 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products10.00%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products21.84%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products29.64%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 64976 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products22.19%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products39.34%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products30.97%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 65057 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products24.34%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products42.28%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products34.29%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 65500 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products16.29%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products31.07%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products28.96%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 65720 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products11.54%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products27.72%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products22.35%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion NA NA Non-coding region v.SL2.50 NC_035963.1 Genomic region 65814 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products9.30%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products21.87%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products18.14%PoorSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1006 CDS v.SL2.50 NC_035963.1 Genomic region 310712 C-to-U NA=>NA L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products34.98%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.31%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products44.55%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1010 CDS v.SL2.50 NC_035963.1 Genomic region 310716 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products34.00%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products44.77%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products43.95%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1016 CDS v.SL2.50 NC_035963.1 Genomic region 310722 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products35.18%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.79%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.03%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 107 CDS v.SL2.50 NC_035963.1 Genomic region 305271 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products30.73%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.80%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products39.63%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1109 CDS v.SL2.50 NC_035963.1 Genomic region 310815 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products31.15%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.22%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.07%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1129 CDS v.SL2.50 NC_035963.1 Genomic region 310835 C-to-U NA=>NA L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products54.86%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.62%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.75%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1148 CDS v.SL2.50 NC_035963.1 Genomic region 310854 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.50%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.21%MediumSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.49%MediumDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1151 CDS v.SL2.50 NC_035963.1 Genomic region 310857 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.68%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.47%MediumSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.48%MediumDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1172 CDS v.SL2.50 NC_035963.1 Genomic region 310878 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products34.36%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.09%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products44.53%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1307 CDS v.SL2.50 NC_035963.1 Genomic region 311013 C-to-U NA=>NA A=>V Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products33.27%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products44.32%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.06%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1355 CDS v.SL2.50 NC_035963.1 Genomic region 311061 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products36.81%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products47.14%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products46.43%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1405 CDS v.SL2.50 NC_035963.1 Genomic region 313738 C-to-U NA=>NA R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products22.31%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products33.91%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products35.77%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1417 CDS v.SL2.50 NC_035963.1 Genomic region 313750 C-to-U NA=>NA H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products21.33%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products32.63%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products34.88%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1433 CDS v.SL2.50 NC_035963.1 Genomic region 313766 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products29.80%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.90%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.71%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 1438 CDS v.SL2.50 NC_035963.1 Genomic region 313771 C-to-U NA=>NA R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products29.08%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.18%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.02%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 29 CDS v.SL2.50 NC_035963.1 Genomic region 305193 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products25.44%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products35.79%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products34.79%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 376 CDS v.SL2.50 NC_035963.1 Genomic region 305540 C-to-U NA=>NA R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products31.31%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.38%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.70%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 436 CDS v.SL2.50 NC_035963.1 Genomic region 305600 C-to-U NA=>NA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products31.78%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.86%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.72%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 437 CDS v.SL2.50 NC_035963.1 Genomic region 305601 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products31.38%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.49%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.24%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 577 CDS v.SL2.50 NC_035963.1 Genomic region 307152 C-to-U NA=>NA L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.93%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.59%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.19%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 608 CDS v.SL2.50 NC_035963.1 Genomic region 307183 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products55.52%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.10%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.60%MediumSimilar36345265
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products32.01%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.23%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.73%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 659 CDS v.SL2.50 NC_035963.1 Genomic region 307234 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products53.91%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.86%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.40%MediumSimilar36345265
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products30.52%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.69%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products39.74%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 767 CDS v.SL2.50 NC_035963.1 Genomic region 307342 C-to-U NA=>NA A=>V Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products32.02%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.28%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.09%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 77 CDS v.SL2.50 NC_035963.1 Genomic region 305241 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products28.68%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products39.36%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products38.65%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad4 952 CDS v.SL2.50 NC_035963.1 Genomic region 307527 C-to-U NA=>NA L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products26.82%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products15.92%PoorDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products16.30%PoorDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad6 161 CDS v.SL2.50 NC_035963.1 Genomic region 291230 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products31.18%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.47%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.27%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad6 191 CDS v.SL2.50 NC_035963.1 Genomic region 291200 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products31.96%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.15%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products41.83%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad6 569 CDS v.SL2.50 NC_035963.1 Genomic region 290822 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products54.71%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.54%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.93%MediumSimilar36345265
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products30.08%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.29%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products40.15%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad7 1050 CDS v.SL2.50 NC_035963.1 Genomic region 353091 C-to-U NA=>NA P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products35.80%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products45.89%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products43.00%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad7 1057 CDS v.SL2.50 NC_035963.1 Genomic region 353084 C-to-U NA=>NA R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products31.91%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products42.33%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products38.64%LowSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad7 200 CDS v.SL2.50 NC_035963.1 Genomic region 357140 C-to-U NA=>NA S=>F NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products36.57%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products47.94%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.54%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad7 209 CDS v.SL2.50 NC_035963.1 Genomic region 357131 C-to-U NA=>NA S=>L NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products34.91%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.90%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.64%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad7 383 CDS v.SL2.50 NC_035963.1 Genomic region 355499 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products38.68%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products49.34%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products49.78%MediumIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad7 578 CDS v.SL2.50 NC_035963.1 Genomic region 355304 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products45.37%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products55.68%MediumIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products53.53%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad9 328 CDS v.SL2.50 NC_035963.1 Genomic region 215190 C-to-U NA=>NA R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products53.05%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products42.43%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products44.25%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion nad9 439 CDS v.SL2.50 NC_035963.1 Genomic region 215301 C-to-U NA=>NA L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products53.61%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products43.56%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.41%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Chloroplast rpoB 2000 CDS v.SL2.50 NC_007898.3 Genomic region 25099 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products0.54%PoorNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products0.43%PoorDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products0.55%PoorSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion rps10 238 CDS v.SL2.50 NC_035963.1 Genomic region 314325 C-to-U NA=>NA R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products26.20%LowNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products37.55%LowIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products38.86%LowIncreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion rps10 302 CDS v.SL2.50 NC_035963.1 Genomic region 315164 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products56.66%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.13%MediumDecreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products46.51%MediumDecreased36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion rps13 287 CDS v.SL2.50 NC_035963.1 Genomic region 375350 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalFruitstage 18Sequencing RT-PCR products50.02%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products60.42%HighIncreased36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Fruitstage 18Sequencing RT-PCR products59.91%MediumSimilar36345265
Solanum lycopersicum Solyc12g014230 Mitochondrion rps13 343 CDS v.SL2.50 NC_035963.1 Genomic region 375294 C-to-U NA=>NA R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Micro-TomWTControlControlNo mutantNormalCarpelstage 18Sequencing RT-PCR products53.07%MediumNone36345265
Micro-Tomslrip1b#1Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#1HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products45.13%MediumSimilar36345265
Micro-Tomslrip1b#2Soft mutationThe soft mutation line with some amino acid changes in SlRIP1b protein, termed here as slrip1b#2HomozygousAnalysis of the growth and developmental traits indicated that slrip1b mutants had comparable plant height, root length, leaf size, number of flowers, and fruit set ratio to WT plants. However, the seed number of the slrip1b mutants was significantly decreased. Notably, flowers of slrip1b mutants exhibited a variety of developmental variations, with an increased number of floral organs, dehiscent stamens, and exposed stigmas. The carpels at stage 18 in slrip1b mutants consisted of larger ovaries and thicker styles compared with WT. Histological examinations of ovaries showed that the number of locules in slrip1b mutants has increased, from two or three in the WT to more than five in the mutant lines. In general, the size of the shoot apical meristems (SAMs) determines the number and shape of floral organs (Xu et al., 2015). We therefore examined SAM size at the transition from vegetative to reproductive growth. As expected, the SAMs of slrip1b mutants were significantly larger than that of WT. Since tomato ovaries will develop into fruits after pollination, we next focused on the fruit development process. As seen in the ovaries, the fruits obtained from slrip1b mutants displayed an increased number of locules, ranging from four to six (instead of two or three in the WT plants). Statistical analysis showed that both the weight and the horizontal diameter of the fruits of slrip1b mutants were notably increased compared to those of the WT plants.Carpelstage 18Sequencing RT-PCR products41.97%MediumDecreased36345265
Last update: Sep 2026 (version 2.0)