Basic Information
Gene Structure
Domain
Regulation&
Interaction
Annotation
Orthologus Group
Pathway
Basic Information
Gene ID
EVM0017155
View in Genome Browser
Position
GWHASIS00001978:12835748-12839775 (+)
4027bp
Gene Type
gene
Gene Description (Protein Product)
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys); tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position
Organism
Acer catalpifolium
Also AS AT4G35910

Gene Structure

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Domain
Database EntryID E-Value Start end InterPro ID Description

Regulation&Interaction
Protein-protein interaction (PPI)
EVM0027179 Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys); tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs; an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position
EVM0027717 Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys); tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP); then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently; a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor
EVM0017881 Monothiol

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Annotation

Orthologous Group
Orthologous ID Species Number All hits in PereRegDB Hits of this species Orthologous Detail


Pathway
Go Pathway KEGG Pathway
GO Term Description GO Category
GO:0002097 tRNA wobble base modification BP
GO:0002098 tRNA wobble uridine modification BP
GO:0002143 tRNA wobble position uridine thiolation BP
GO:0003674 molecular_function MF
GO:0003824 catalytic activity MF
GO:0005575 cellular_component CC
GO:0005622 intracellular anatomical structure CC
GO:0005623 obsolete cell CC
GO:0005737 cytoplasm CC
GO:0005829 cytosol CC
GO:0006139 nucleobase-containing compound metabolic process BP
GO:0006396 RNA processing BP
GO:0006399 tRNA metabolic process BP
GO:0006400 tRNA modification BP
GO:0006725 cellular aromatic compound metabolic process BP
GO:0006807 nitrogen compound metabolic process BP
GO:0007275 multicellular organism development BP
GO:0008033 tRNA processing BP
GO:0008150 biological_process BP
GO:0008152 metabolic process BP
GO:0009451 RNA modification BP
GO:0009653 anatomical structure morphogenesis BP
GO:0009791 post-embryonic development BP
GO:0009987 cellular process BP
GO:0010015 root morphogenesis BP
GO:0010101 post-embryonic root morphogenesis BP
GO:0010102 lateral root morphogenesis BP
GO:0010311 lateral root formation BP
GO:0010467 gene expression BP
GO:0016070 RNA metabolic process BP
GO:0016740 transferase activity MF
GO:0016782 transferase activity, transferring sulphur-containing groups MF
GO:0016783 sulfurtransferase activity MF
GO:0022622 root system development BP
GO:0032501 multicellular organismal process BP
GO:0032502 developmental process BP
GO:0034227 tRNA thio-modification BP
GO:0034470 ncRNA processing BP
GO:0034641 cellular nitrogen compound metabolic process BP
GO:0034660 ncRNA metabolic process BP
GO:0043170 macromolecule metabolic process BP
GO:0043412 macromolecule modification BP
GO:0044237 cellular metabolic process BP
GO:0044238 primary metabolic process BP
GO:0044424 obsolete intracellular part CC
GO:0044444 obsolete cytoplasmic part CC
GO:0044464 obsolete cell part CC
GO:0046483 heterocycle metabolic process BP
GO:0048364 root development BP
GO:0048527 lateral root development BP
GO:0048528 post-embryonic root development BP
GO:0048646 anatomical structure formation involved in morphogenesis BP
GO:0048731 system development BP
GO:0048856 anatomical structure development BP
GO:0071704 organic substance metabolic process BP
GO:0090304 nucleic acid metabolic process BP
GO:0090696 post-embryonic plant organ development BP
GO:0090697 post-embryonic plant organ morphogenesis BP
GO:0090698 post-embryonic plant morphogenesis BP
GO:0099402 plant organ development BP
GO:1901360 organic cyclic compound metabolic process BP
GO:1905392 plant organ morphogenesis BP
GO:1905393 plant organ formation BP
KEGG Term Name Description
map04122 Sulfur relay system Ubiquitin and ubiquitin-like proteins (Ubls) are signalling messengers that control many cellular functions, such as cell proliferation, apoptosis, and DNA repair. It is suggested that Ub-protein modification evolved from prokaryotic sulfurtransfer systems. Molybdenum cofactor (Moco) and thiamin are sulfur-containing cofactors whose biosynthesis includes a key sulfur transfer step that uses unique sulfur carrier proteins, MoaD and ThiS. Ubiquitin, MoaD, and ThiS are all structurally related proteins whose C-termini are activated through adenylation by homologous E1-like enzymes. s2T biosynthesis may share similar chemistry with Moco and thiamin synthesis. In Saccharomyces cerevisiae, Urm1 and Uba4 function as part of a ubl protein conjugation system, though they have sequence homology to bacterial sulfur-transfer enzymes and the ability to function in sulfur transfer.