DEK53 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G480380

Mitochondrion

atp1

1449

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	6.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	5.67%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	6.67%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	2.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

atp1

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	40.67%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	26.67%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	38.00%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	17.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

atp4

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	95.00%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	81.00%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	92.67%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	81.00%	High	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

atp6

1050

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	14.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	7.33%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	29.67%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	19.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

atp8

123

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	82.67%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	68.33%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	81.67%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	65.67%	High	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

atp8

436

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	78.67%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	69.33%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	81.67%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	64.00%	High	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

atp8

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	13.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	9.33%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	6.67%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	2.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmB

424

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	7.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	0.00%	Unedited	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	13.00%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	0.00%	Unedited	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmC

421

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	16.67%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	22.33%	Low	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	18.31%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	25.45%	Low	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmC

436

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	16.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	20.33%	Low	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	11.04%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	19.14%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmC

446

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	21.33%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	11.33%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	17.67%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	15.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmC

497

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	8.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	13.00%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	9.01%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	16.66%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmC

499

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	9.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	13.67%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	13.75%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	19.66%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmC

568

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	9.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	14.00%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	12.53%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	19.65%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmC

673

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	13.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	21.33%	Low	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	15.28%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	26.53%	Low	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmFC

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	19.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	9.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	8.00%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	5.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmFn

1492

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	16.67%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	5.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	38.33%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	12.67%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmFn

1505

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	73.67%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	63.00%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	76.00%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	64.33%	High	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmFn

1709

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	28.33%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	19.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	15.33%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	9.67%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmFn

318

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	25.33%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	18.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	48.33%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	27.33%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmFn

762

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	20.67%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	17.67%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	33.00%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	24.00%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

ccmFn

795

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	40.67%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	32.00%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	24.00%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	19.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

cob

725

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	74.33%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	61.67%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	82.67%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	71.67%	High	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

cox2

162

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	39.00%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	32.00%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	29.00%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	22.33%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

cox2

482

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	69.33%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	60.00%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	72.67%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	55.00%	Medium	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

cox2

620

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	82.33%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	66.00%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	90.33%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	72.00%	High	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

cox2

638

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	70.00%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	37.00%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	67.00%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	28.00%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

matR

1905

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	10.67%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	18.00%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	46.21%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	56.21%	Medium	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

matR

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	7.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	19.67%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	53.41%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	69.67%	High	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

149

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	17.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	10.33%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	8.33%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	4.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

205

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	14.67%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	10.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	8.33%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	4.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

227

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	12.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	6.67%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	7.67%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	2.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

355

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	7.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	3.67%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	6.67%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	2.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

373

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	35.33%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	22.33%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	26.67%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	9.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

434

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	21.33%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	18.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	14.67%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	7.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

532

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	45.67%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	35.33%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	52.33%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	28.67%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

mttB

575

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	11.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	8.67%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	10.33%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	5.00%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad1

537

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	26.67%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	51.00%	Medium	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	37.12%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	59.61%	Medium	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad1

835

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	9.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	13.00%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	7.61%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	12.97%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad2

1057

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	23.67%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	33.00%	Low	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	11.13%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	12.45%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad2

1336

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	8.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	6.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	21.67%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	15.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad3

137

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	56.67%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	63.00%	High	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	56.29%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	72.63%	High	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad3

146

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	31.00%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	17.33%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	29.00%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	20.67%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad3

190

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	24.00%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	32.33%	Low	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	32.30%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	44.07%	Medium	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad3

344

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	33.33%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	21.00%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	42.67%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	25.00%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad3

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	13.67%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	19.67%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	10.45%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	14.77%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad5

1183

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	14.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	10.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	22.67%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	12.67%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad6

146

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	93.00%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	79.00%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	88.33%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	74.00%	High	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad6

306

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	6.67%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	10.00%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	5.29%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	12.08%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad6

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	16.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	8.00%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	5.67%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	2.33%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad7

531

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	8.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	11.33%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	6.32%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	7.27%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad7

963

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	40.67%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	33.00%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	39.00%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	32.00%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad9

113

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	66.00%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	55.67%	Medium	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	65.33%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	45.33%	Medium	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad9

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	74.33%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	57.00%	Medium	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	71.67%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	57.33%	Medium	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad9

223

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	73.00%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	65.00%	High	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	65.00%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	45.00%	Medium	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

nad9

356

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	56.33%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	39.67%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	52.00%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	33.00%	Low	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rpl16

444

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	57.33%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	80.67%	High	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	47.18%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	73.33%	High	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps2B

510

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	16.67%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	37.00%	Low	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	19.40%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	38.33%	Low	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps2B

526

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	6.33%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	17.00%	Poor	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	12.12%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	15.44%	Poor	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps3

1066

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	15.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	28.00%	Low	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	21.90%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	38.73%	Low	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps3

497

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	34.33%	Low	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	62.67%	High	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	40.11%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	67.43%	High	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps3

707

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	41.00%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	68.33%	High	Increased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	36.38%	Low	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	72.81%	High	Increased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps4

290

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	69.67%	High	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	54.67%	Medium	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	64.67%	High	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	41.33%	Medium	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps4

474

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	14.00%	Poor	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	8.67%	Poor	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	15.00%	Poor	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	9.67%	Poor	Decreased	32710615

Zea mays

GRMZM2G480380

Mitochondrion

rps4

944

CDS

B73
W22

NA
W22

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT-ref	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	48.67%	Medium	None	32710615
B73	dek53-ref	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 53 bp downstream of the start codon	Homozygous	At maturity, homozygous dek53-ref kernels were misshapen, and embryo lethal, as no mutant plants were able to germinate. The hundred-kernel weight of dek53-ref kernels was only 18% of that of the WT	Kernel	15 DAP	STS-PCRseq	34.67%	Low	Decreased	32710615
W22	WT-mu1	Control	Control	No mutant	Normal	Kernel	15 DAP	STS-PCRseq	50.33%	Medium	None	32710615
W22	dek53-mu1	Insertion	The maize dek53-ref is a dek mutant isolated from F2 populations containing active MuDR. The Mu insertion was located 177 bp downstream of the start codon	Homozygous	Homozygous dek53-mu1 kernels displayed a small and collapsed phenotype with delayed development	Kernel	15 DAP	STS-PCRseq	24.00%	Low	Decreased	32710615

Editing Factor:	DEK53
Synonym:	Defective Kernel 53
Description:	Biochemical analysis of mitochondrial protein complexes revealed a significant reduction in the assembly of mitochondrial complex III in dek53; Transmission electron microscopic examination showed severe morphological defects of mitochondria in dek53 endosperm cells; In addition, yeast two-hybrid and luciferase complementation imaging assays indicated that DEK53 can interact with the mitochondrion-targeted non-PPR RNA editing factor ZmMORF1, suggesting that DEK53 might be a functional component of the organellar RNA editosome
Protein Family:	PPR
Subclass:	E
Physical Interaction:	ZmMORF1
Construct Structure:	PLS-E1-E2
Gene ID & Species:	GRMZM2G480380 (Zea mays)
Edited Gene(s):	nad2 nad3 nad5 nad6 nad7 nad9 rps4 atp1 atp4 atp6 atp8 ccmB ccmC ccmFC ccmFn cob cox2 mttB nad1 rps2B rps3 rpl16 matR
Editing Type(s):	C-to-U (260)
Publication(s):	[1] Maize pentatricopeptide repeat protein DEK53 is required for mitochondrial RNA editing at multiple sites and seed development., Journal of experimental botany, 2020. [PMID=32710615]

Summary

Editing Details