ORRM6 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G73530

Plastid

accD

1568

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	76.92%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	74.07%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Chloroplast
Plastid

accD

794

CDS

C-to-U

UCG=>UUG
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	NA	Low	Decreased	28213559
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	NA	NA	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	5 weeks	STS-PCRseq	NA	Low	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	NA	Low	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	NA	NA	Decreased	28213559
NA	WT	Control	Control	Control	Normal	Leaf	5 weeks	STS-PCRseq	100.00%	Complete	None	28213559
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Sanger sequencing	100.00%	Complete	None	28213559
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	28213559
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM7 gene	Knockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	30.70%	Low	Decreased	28387567
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM10 gene	Knockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	29.00%	Low	Decreased	28387567
NA	WT	Control	Control	Control	Normal	Leaf	NA	Sanger sequencing	85.80%	High	None	28387567
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	27.27%	Low	Decreased	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	29.41%	Low	Decreased	32259001

Arabidopsis thaliana

AT1G73530

Plastid

atpF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

matK

640

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	86.96%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.48%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

1255

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

1481

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

149

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

467

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

586

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

746

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

830

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

872

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	50.00%	Medium	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	62.50%	High	Increased	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

383

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

674

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Increased	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Increased	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

878

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

887

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhF

290

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhG

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

petL

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	80.00%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	88.89%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

psbE

214

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Chloroplast
Plastid

psbF

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	0.00%	Unedited	Absent	28213559
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	5 weeks	STS-PCRseq	NA	Poor	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	0.00%	Unedited	Absent	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	28213559
NA	WT	Control	Control	Control	Normal	Leaf	5 weeks	STS-PCRseq	100.00%	Complete	None	28213559
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Sanger sequencing	100.00%	Complete	None	28213559
NA	WT	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	28213559
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM6 gene	Knockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	6.80%	Poor	Decreased	28387567
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM9 gene	Knockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	6.30%	Poor	Decreased	28387567
NA	WT	Control	Control	Control	Normal	Leaf	NA	Sanger sequencing	97.10%	High	None	28387567
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT1G73530

Plastid

psbZ

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	94.12%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	89.47%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpl23

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	13.33%	Poor	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	14.29%	Poor	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.00%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.00%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoB

2432

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.91%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	83.33%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoB

338

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	95.00%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	95.00%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoB

551

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoC1

488

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	39.13%	Low	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	39.13%	Low	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rps12

Intron

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	15.00%	Poor	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	15.00%	Poor	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rps14

149

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	77.78%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	75.00%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rps14

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	91.67%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.91%	High	Similar	32259001

Editing Factor:	ORRM6
Synonym:	ORGANELLE RNA RECOGNITION MOTIF PROTEIN 6
Description:	Encoding an organelle RNA recognition motif protein required for photosystem II subunit psbF transcript editing
Protein Family:	ORRM
Subclass:	NA
Construct Structure:	NA, PTP-ORRM
Gene ID & Species:	AT1G73530 (Arabidopsis thaliana)
Edited Gene(s):	psbF accD clpP matK ndhB ndhD ndhG rpoA rpoB rps12 rps14 atpF ndhF petL psbE psbZ rpl23 rpoC1
Editing Type(s):	C-to-U (90)
Publication(s):	[1] Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in the plastid., Plant direct, 2020. [PMID=32259001] [2] Whole-transcriptome RNA-seq, gene set enrichment pathway analysis, and exon coverage analysis of two plastid RNA editing mutants., Plant signaling & behavior, 2017. [PMID=28387567] [3] An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit Transcript Editing., Plant physiology, 2017. [PMID=28213559]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details