ORRM6 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G73530

Plastid

accD

1568

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	76.92%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	74.07%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

accD

794

CDS

TAIR (v10)

NC_000932.1

Genomic position

57868

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM7 gene	Knockout	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	30.70%	Low	Decreased	28387567
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM10 gene	Knockout	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	29.00%	Low	Decreased	28387567
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Sanger sequencing	85.80%	High	None	28387567

Arabidopsis thaliana

AT1G73530

Chloroplast
Plastid

accD

794

CDS

C-to-U

UCG=>UUG
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	NA	Low	Decreased	28213559
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	NA	NA	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	5 weeks	STS-PCRseq	NA	Low	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	NA	Low	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	NA	NA	Decreased	28213559
NA	WT	Control	Control	No mutant	Normal	Leaf	5 weeks	STS-PCRseq	100.00%	Complete	None	28213559
NA	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Sanger sequencing	100.00%	Complete	None	28213559
NA	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	28213559
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	27.27%	Low	Decreased	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	29.41%	Low	Decreased	32259001

Arabidopsis thaliana

AT1G73530

Plastid

atpF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

matK

640

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	86.96%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.48%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

1255

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

1481

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

149

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

467

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

586

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

746

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

830

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhB

872

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	50.00%	Medium	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	62.50%	High	Increased	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

383

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

674

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Increased	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Increased	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

878

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhD

887

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhF

290

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

ndhG

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

petL

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	80.00%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	88.89%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

psbE

214

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

psbF

CDS

TAIR (v10)

NC_000932.1

Genomic position

63985

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM6 gene	Knockout	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	6.80%	Poor	Decreased	28387567
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA insertions in the first intron of the ORRM9 gene	Knockout	Reduced photosystem II (PSII) activity and smaller plant and leaf sizes	Leaf	NA	Sanger sequencing	6.30%	Poor	Decreased	28387567
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Sanger sequencing	97.10%	High	None	28387567

Arabidopsis thaliana

AT1G73530

Chloroplast
Plastid

psbF

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	0.00%	Unedited	Absent	28213559
SAIL_763_A05	orrm6-1	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	5 weeks	STS-PCRseq	NA	Poor	Decreased	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Sanger sequencing	0.00%	Unedited	Absent	28213559
WiscDsLox485-488P23	orrm6-2	T-DNA Insertion	T-DNA is inserted in the first intron	Knockout; Homozygous	Pale green pigmentation, smaller leaf and plant sizes, and retarded growth	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	28213559
NA	WT	Control	Control	No mutant	Normal	Leaf	5 weeks	STS-PCRseq	100.00%	Complete	None	28213559
NA	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Sanger sequencing	100.00%	Complete	None	28213559
NA	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	28213559
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT1G73530

Plastid

psbZ

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	94.12%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	89.47%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpl23

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	13.33%	Poor	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	14.29%	Poor	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.00%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.00%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoB

2432

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.91%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	83.33%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoB

338

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	95.00%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	95.00%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoB

551

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rpoC1

488

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	39.13%	Low	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	39.13%	Low	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rps12

Intron

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	15.00%	Poor	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	15.00%	Poor	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rps14

149

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	77.78%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	75.00%	High	Similar	32259001

Arabidopsis thaliana

AT1G73530

Plastid

rps14

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm6-1	T-DNA insertion lines orrm6-1 (SAIL_763_A05)	NA	Homozygous	The orrm6-1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	91.67%	High	Similar	32259001
Columbia	orrm6-2	T-DNA insertion lines orrm6-2 (WiscDsLox485-488P23)	NA	Homozygous	The orrm6-2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.	Leaf	4-week	RT-PCR and Sanger sequencing	90.91%	High	Similar	32259001

Editing Factor:	ORRM6
Synonym:	ORGANELLE RNA RECOGNITION MOTIF PROTEIN 6
Description:	Unlike ORRM1, the other known ORRM plastid editing factor, ORRM6, does not contain RNA editing interacting protein/multiple organellar RNA editing factor (RIP/MORF) boxes, which are required for ORRM1 to interact with site-specific pentatricopeptide repeat protein editing factors; While some plastid RRM proteins are involved in other forms of RNA processing and translation, the primary function of ORRM6 is evidently to mediate psbF-C77 editing, like the essential site-specific pentatricopeptide repeat protein LOW PSII ACCUMULATION66; Stable expression in the orrm6 mutants of a nucleus-encoded, plastid-targeted PsbF protein from a psbF gene carrying a T at nucleotide 77 significantly increases leaf and plant sizes, chlorophyll content, and PSII activity; Sanger sequencing of reverse transcription-PCR products of plastid transcripts revealed 2 plastid RNA editing sites that are substantially affected in the orrm6 mutants: psbF-C77 and accD-C794; Gene set enrichment pathway analysis showed that expression of multiple gene sets involved in photosynthesis, especially photosynthetic electron transport, is significantly upregulated in both orrm6 mutants; The upregulation could be a mechanism to compensate for the reduced PSII electron transport rate in the orrm6 mutants
Protein Family:	Organelle RNA recognition motif-containing (ORRM)
Subclass:	NA
Physical Interaction:	OZ1; ORRM6; RIP1/MORF8; RIP2/MORF2; RIP9/MORF9, RIP1/MORF8; RIP2/MORF2; RIP9/MORF9, RIP1/MORF8; RIP2/MORF2; RIP9/MORF9; OZ1; ORRM6
Construct Structure:	PTP-ORRM
Gene ID & Species:	AT1G73530 (Arabidopsis thaliana)
Edited Gene(s):	psbF accD clpP matK ndhB ndhD ndhG rpoA rpoB rps12 rps14 atpF ndhF petL psbE psbZ rpl23 rpoC1
Editing Type(s):	C-to-U (90)
Publication(s):	[1] Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in the Arabidopsis thaliana plastid, Plant Direct, 2020. [doi:10.1002/pld3.213] [2] Whole-Transcriptome RNA-seq, Gene Set Enrichment Pathway Analysis, and Exon Coverage Analysis of Two Plastid RNA Editing Mutants, Plant Signaling & Behavior, 2017. [doi:10.1080/15592324.2017.1312242] [3] An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing, Plant Physiology, 2017. [doi:10.1104/pp.16.01623]

Summary

Editing Details