MORF2 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G33430

Chloroplast

accD

794

CDS

C-to-U

UCG=>UUG
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	4.00%	Poor	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	89.00%	High	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	82.35%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	82.35%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	50.00%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	80.00%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	90.00%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

accD

3'UTR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	40.00%	Medium	Increased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	20.00%	Low	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	50.00%	Medium	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	72.22%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	50.00%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	54.17%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	83.12%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

atpF

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	10.00%	Poor	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	88.60%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	89.62%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	84.00%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	86.79%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	91.20%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

clpP

559

CDS

C-to-U

CAU=>UAU
NA=>NA

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	55.00%	Medium	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	68.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	65.00%	High	None	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	79.00%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	76.41%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	74.94%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	78.52%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	73.46%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

matK

706

CDS

C-to-U

CAU=>UAU
NA=>NA

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	45.00%	Medium	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	70.00%	High	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	69.70%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	60.10%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	65.77%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	77.03%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	77.21%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhB

1255

CDS

C-to-U

CAU=>UAU
NA=>NA

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	10.00%	Poor	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	WT + Dex (5 μM)	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing amplified PCR fragments	90.00%	High	None	37056496
Columbia (Col-0)	P1-12 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	50.00%	Medium	Decreased	37056496
Columbia (Col-0)	P1-10 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	90.20%	High	Similar	37056496
Columbia (Col-0)	P1-54 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	23.21%	Low	Decreased	37056496
Columbia (Col-0)	P1-12 + Dex (0.25 μM)	Treated with Dex	Treated with 0.25 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	91.23%	High	Similar	37056496
SALK_094930	morf2-2	T-DNA insertion	T-DNA insertion in an intron	Homozygous	Retarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germination	Seedling	7-d-old	Sequencing amplified PCR fragments	0.00%	Unedited	Absent	37056496
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	98.29%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.75%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.38%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	97.62%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	91.50%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhB

1481

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	10.00%	Poor	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	86.15%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	87.03%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.59%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	83.22%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	83.47%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhB

149

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	90.00%	High	None	22411807
Columbia (Col-0)	WT + Dex (5 μM)	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing amplified PCR fragments	90.16%	High	None	37056496
Columbia (Col-0)	P1-12 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	30.16%	Low	Decreased	37056496
Columbia (Col-0)	P1-10 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	87.10%	High	Similar	37056496
Columbia (Col-0)	P1-54 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	73.68%	High	Decreased	37056496
Columbia (Col-0)	P1-12 + Dex (0.25 μM)	Treated with Dex	Treated with 0.25 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	86.67%	High	Similar	37056496
SALK_094930	morf2-2	T-DNA insertion	T-DNA insertion in an intron	Homozygous	Retarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germination	Seedling	7-d-old	Sequencing amplified PCR fragments	0.00%	Unedited	Absent	37056496
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	86.02%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	79.03%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	77.61%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	79.53%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	62.31%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

ndhB

467

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	5.00%	Poor	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	90.00%	High	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	20.00%	Low	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	75.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	75.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment. MORF2 is overexpressed and fused with 3xHA-3xFLAG tags in the wild-type Col6-3 background.	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	15.00%	Poor	NA	30988197
Columbia (Col-0)	WT + Dex (5 μM)	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing amplified PCR fragments	92.16%	High	None	37056496
Columbia (Col-0)	P1-12 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	14.55%	Poor	Decreased	37056496
Columbia (Col-0)	P1-10 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	87.27%	High	Similar	37056496
Columbia (Col-0)	P1-54 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	90.91%	High	Similar	37056496
Columbia (Col-0)	P1-12 + Dex (0.25 μM)	Treated with Dex	Treated with 0.25 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	84.91%	High	Similar	37056496
SALK_094930	morf2-2	T-DNA insertion	T-DNA insertion in an intron	Homozygous	Retarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germination	Seedling	7-d-old	Sequencing amplified PCR fragments	0.00%	Unedited	Absent	37056496
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	82.86%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	81.03%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	79.79%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	82.95%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	78.95%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhB

586

CDS

C-to-U

CAU=>UAU
NA=>NA

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	20.00%	Low	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	WT + Dex (5 μM)	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	None	37056496
Columbia (Col-0)	P1-12 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	13.73%	Poor	Decreased	37056496
Columbia (Col-0)	P1-10 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	Similar	37056496
Columbia (Col-0)	P1-54 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	Similar	37056496
Columbia (Col-0)	P1-12 + Dex (0.25 μM)	Treated with Dex	Treated with 0.25 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	Similar	37056496
SALK_094930	morf2-2	T-DNA insertion	T-DNA insertion in an intron	Homozygous	Retarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germination	Seedling	7-d-old	Sequencing amplified PCR fragments	6.74%	Poor	Decreased	37056496
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	89.32%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	84.54%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	83.03%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	88.70%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	87.36%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhB

746

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	86.09%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	88.72%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	85.71%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	88.85%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	85.39%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhB

830

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	WT + Dex (5 μM)	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	None	37056496
Columbia (Col-0)	P1-12 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	72.73%	High	Decreased	37056496
Columbia (Col-0)	P1-10 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	Similar	37056496
Columbia (Col-0)	P1-54 + Dex (5 μM)	Treated with Dex	Treated with 5 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	Similar	37056496
Columbia (Col-0)	P1-12 + Dex (0.25 μM)	Treated with Dex	Treated with 0.25 μM Dex	NA	NA	Seedling	7-d-old	Sequencing amplified PCR fragments	100.00%	Complete	Similar	37056496
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	90.02%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	90.74%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	87.70%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	84.08%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	84.57%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

ndhB

836

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	40.00%	Medium	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	70.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	60.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	10.00%	Poor	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	79.44%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	85.59%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	78.06%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	73.46%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	65.45%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhB

872

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	85.00%	High	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	86.68%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	81.52%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	82.91%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	77.73%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	87.12%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhD

CDS

C-to-U

ACG=>AUG
NA=>NA

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	45.00%	Medium	None	22411807
NA	WT	Wild Type	Wild Type	No mutant	Normal	NA	NA	NA	NA	NA	NA	30471730
Columbia (Col-0)	ProMORF2: MORF2-4xMYC	Arabidopsis Col-0 transgenic plants expressing MORF2-4xMYC.	To obtain MYC-tagged transgenic plants for MORF2 analysis, constructs expressing genes encoding MORF2-4xMYC fusion proteins, driven by the corresponding native promoters were transformed into wild-typ	NA	Normal	NA	NA	NA	NA	NA	NA	30471730
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	22.83%	Low	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	29.07%	Low	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	27.87%	Low	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	21.10%	Low	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	12.87%	Poor	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhD

383

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	90.00%	High	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	74.22%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	56.57%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	53.85%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	69.07%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	83.70%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhD

674

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	74.32%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	62.50%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	58.72%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	79.79%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	80.14%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

ndhD

878

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	60.00%	High	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	78.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	75.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	20.00%	Low	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	62.91%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	53.91%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	73.25%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	69.09%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	74.32%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhD

887

CDS

C-to-U

CCC=>CUC
NA=>NA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	5.00%	Poor	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	69.27%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	64.60%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	77.42%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	72.90%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	78.24%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

ndhF

290

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	75.00%	High	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	60.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	70.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	10.00%	Poor	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	4.70%	Poor	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	4.85%	Poor	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	4.00%	Poor	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	7.52%	Poor	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	12.38%	Poor	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

ndhG

CDS

C-to-U

UCC=>UUC
NA=>NA

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	40.00%	Medium	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	70.18%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	72.52%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	75.86%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	60.00%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	73.42%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

petL

CDS

C-to-U

CCU=>CUU
NA=>NA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	65.00%	High	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	43.64%	Medium	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	26.00%	Low	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	42.25%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	53.57%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	71.43%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

psbE

214

CDS

C-to-U

CCU=>UCU
NA=>NA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	30.00%	Low	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	99.42%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	99.50%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	99.26%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	97.73%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	95.45%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

psbF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807

Arabidopsis thaliana

AT2G33430

Chloroplast

psbF

CDS

C-to-U

NA=>NA

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.17%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	98.12%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	99.26%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.04%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	95.63%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

psbZ

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	92.00%	High	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	72.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	75.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	15.00%	Poor	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	80.28%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	76.63%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	76.48%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	76.39%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	66.71%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

rpl23

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	70.00%	High	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	91.97%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	92.88%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	91.96%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	91.85%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	95.40%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

rpoA

200

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	Increased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	87.75%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	89.06%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	86.41%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	90.18%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	86.94%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

rpoB

2432

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	90.00%	High	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	65.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	70.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	55.00%	Medium	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	91.91%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.13%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	85.14%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	95.56%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.90%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

rpoB

338

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	70.00%	High	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	90.00%	High	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	85.00%	High	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	60.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	75.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	55.00%	Medium	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	84.82%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	84.50%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	79.22%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	82.83%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.85%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

rpoB

551

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	90.00%	High	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	85.00%	High	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	60.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	70.00%	High	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	25.00%	Low	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	94.25%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	92.11%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	90.74%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	91.36%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	95.45%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Plastid
Chloroplast

rpoC1

488

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	60.00%	High	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	25.00%	Low	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	30.00%	Low	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	40.00%	Medium	NA	30988197
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	33.33%	Low	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	55.26%	Medium	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	35.56%	Low	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	35.48%	Low	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	32.61%	Low	None	37056496

Arabidopsis thaliana

AT2G33430

Plastid

rps12

CDS

C-to-U

CAA/CAG=>UAA/UAG

G=>Stop codon

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	NULL	NA	Seedling	NA	Direct Sequencing of PCR Products	35.00%	Low	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	50.00%	Medium	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedling	NA	Direct Sequencing of PCR Products	55.00%	Medium	None	30988197
Col6-3	MORF2OX	NF treatment; Overexpression	A bleaching herbicide norflurazon (NF) treatment	NULL	Seedling is pale and die	Seedling	NA	Direct Sequencing of PCR Products	10.00%	Poor	NA	30988197

Arabidopsis thaliana

AT2G33430

Chloroplast

rps12

Intron

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	15.00%	Poor	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	20.00%	Low	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	13.92%	Poor	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	17.97%	Poor	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	18.25%	Poor	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	20.95%	Low	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	15.50%	Poor	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

rps14

149

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	95.00%	High	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	94.41%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	95.02%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	93.50%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	92.10%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	89.52%	High	None	37056496

Arabidopsis thaliana

AT2G33430

Chloroplast

rps14

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22411807
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807
Columbia (Col-0)	KRAB (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	96.27%	High	None	37056496
Columbia (Col-0)	KRAB (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	97.80%	High	None	37056496
Columbia (Col-0)	P1-12 (+DMSO)	Treated with DMSO	NA	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	95.29%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.025 mM Dex)	Treated with Dex	Treated with 0.025 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	94.88%	High	None	37056496
Columbia (Col-0)	P1-12 (+0.25 mM Dex)	Treated with Dex	Treated with 0.25 mM Dex	NA	NA	Seedling	8-d-old	Sequencing amplified PCR fragments	90.17%	High	None	37056496

Editing Factor:	MORF2
Synonym:	DAL; DAL1; DIFFERENTIATION AND GREENING-LIKE, DIFFERENTIATION AND GREENING-LIKE 1; MULTIPLE ORGANELLAR RNA EDITING FACTOR 2
Description:	Encoding an editing factor involved at almost all RNA editing sites in chloroplasts; MORF2-MORF9 heterodimer is the core members of editosomes in chloroplasts, while they are not responsible for RNA editing site recognition; Reducing MORF2 by iCRISPRi stimulated the expression of stress responsive genes, triggered plastidial retrograde signaling, repressed ethylene signaling and skotomorphogenesis, and increased accumulation of hydrogen peroxide; These findings along with previous discoveries suggest that MORF2 is an effective regulator involved in plastidial metabolic pathways whose reduction can readily activate multiple retrograde signaling molecules possibly involving reactive oxygen species to adjust plant growth
Protein Family:	MORF
Subclass:	NA
Construct Structure:	NA
Gene ID & Species:	AT2G33430 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD psbE psbF atpF clpP accD matK ndhF ndhG petL rpoB rps14 rpoA psbZ rps12 rpl23 rpoC1
Editing Type(s):	C-to-U (310)
Publication(s):	[1] MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing., Frontiers in plant science, 2023. [PMID=37056496] [2] GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling., Proceedings of the National Academy of Sciences of the United States of America, 2019. [PMID=30988197] [3] MORF2 tightly associates with MORF9 to regulate chloroplast RNA editing in Arabidopsis., Plant science : an international journal of experimental plant biology, 2019. [PMID=30471730] [4] Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants., Proceedings of the National Academy of Sciences of the United States of America, 2012. [PMID=22411807]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details