MORF2 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MORF2
Synonym: DAL; DAL1; DIFFERENTIATION AND GREENING-LIKE, DIFFERENTIATION AND GREENING-LIKE 1; MULTIPLE ORGANELLAR RNA EDITING FACTOR 2
Description: Encoding an editing factor involved at almost all RNA editing sites in chloroplasts; MORF2-MORF9 heterodimer is the core members of editosomes in chloroplasts, while they are not responsible for RNA editing site recognition; Reducing MORF2 by iCRISPRi stimulated the expression of stress responsive genes, triggered plastidial retrograde signaling, repressed ethylene signaling and skotomorphogenesis, and increased accumulation of hydrogen peroxide; These findings along with previous discoveries suggest that MORF2 is an effective regulator involved in plastidial metabolic pathways whose reduction can readily activate multiple retrograde signaling molecules possibly involving reactive oxygen species to adjust plant growth
Protein Family: MORF
Subclass: NA
Physical Interaction: GUN1, MORF1; MORF9; MEF19; MEF21, MORF9; PDM1, OTP81; OTP84; YS1; GUN1
Construct Structure: NA
Gene ID & Species: AT2G33430 (Arabidopsis thaliana)
Edited Gene(s): ndhB    ndhD    psbE    psbF    atpF    clpP    accD    matK    ndhF    ndhG    petL    rpoB    rps14    rpoA    psbZ    rps12    rpl23    rpoC1
Editing Type(s): C-to-U (310)
Publication(s): [1] MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing., Frontiers in plant science, 2023. [PMID=37056496]
[2] GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling., Proceedings of the National Academy of Sciences of the United States of America, 2019. [PMID=30988197]
[3] MORF2 tightly associates with MORF9 to regulate chloroplast RNA editing in Arabidopsis., Plant science : an international journal of experimental plant biology, 2019. [PMID=30471730]
[4] Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants., Proceedings of the National Academy of Sciences of the United States of America, 2012. [PMID=22411807]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G33430 Chloroplast accD 794 CDS NA
TAIR10
NA NA
Genomic position
NA
57868
C-to-U UCG=>UUG
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products4.00%PoorDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products89.00%HighNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments82.35%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments82.35%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments50.00%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments80.00%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.00%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast accD NA 3'UTR NA
TAIR10
NA Genomic position 58642 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products40.00%MediumIncreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products20.00%LowNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments50.00%MediumNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments72.22%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments50.00%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments54.17%MediumNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.12%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast atpF 92 CDS NA
TAIR10
NA NA
Genomic position
NA
12707
C-to-U CCA=>CUA
NA=>NA
P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products10.00%PoorDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments88.60%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments89.62%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments84.00%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments86.79%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.20%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
clpP 559 CDS NA
TAIR10
NA NA
Genomic position
NA
69942
C-to-U CAU=>UAU
NA=>NA
H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products60.00%HighDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products80.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products55.00%MediumNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products68.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products65.00%HighNone30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.00%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments76.41%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments74.94%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments78.52%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments73.46%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast matK 706 CDS NA
TAIR10
NA NA
Genomic position
NA
293167
C-to-U CAU=>UAU
NA=>NA
H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products45.00%MediumDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products70.00%HighNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments69.70%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments60.10%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments65.77%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments77.03%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments77.21%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 1255 CDS NA
TAIR10
NA NA
Genomic position
NA
95225
C-to-U CAU=>UAU
NA=>NA
H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products10.00%PoorDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments90.00%HighNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments50.00%MediumDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments90.20%HighSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments23.21%LowDecreased37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments91.23%HighSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments0.00%UneditedAbsent37056496
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments98.29%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.75%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.38%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments97.62%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.50%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 1481 CDS NA
TAIR10
NA NA
Genomic position
NA
94999
C-to-U CCA=>CUA
NA=>NA
P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products10.00%PoorDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.15%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments87.03%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.59%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.22%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.47%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 149 CDS NA
TAIR10
NA NA
Genomic position
NA
97016
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments90.16%HighNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments30.16%LowDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments87.10%HighSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments73.68%HighDecreased37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments86.67%HighSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments0.00%UneditedAbsent37056496
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.02%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments79.03%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments77.61%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments79.53%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments62.31%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhB 467 CDS NA
TAIR10
NA NA
Genomic position
NA
96698
C-to-U CCA=>CUA
NA=>NA
P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products5.00%PoorDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products20.00%LowNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatment. MORF2 is overexpressed and fused with 3xHA-3xFLAG tags in the wild-type Col6-3 background.NULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products15.00%PoorNA30988197
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments92.16%HighNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments14.55%PoorDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments87.27%HighSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments90.91%HighSimilar37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments84.91%HighSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments0.00%UneditedAbsent37056496
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments82.86%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments81.03%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.79%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments82.95%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments78.95%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 586 CDS NA
TAIR10
NA NA
Genomic position
NA
96579
C-to-U CAU=>UAU
NA=>NA
H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products20.00%LowDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments13.73%PoorDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments6.74%PoorDecreased37056496
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments89.32%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.54%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments83.03%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments88.70%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments87.36%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 746 CDS NA
TAIR10
NA NA
Genomic position
NA
96419
C-to-U UCU=>UUU
NA=>NA
S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.09%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments88.72%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments85.71%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments88.85%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments85.39%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 830 CDS NA
TAIR10
NA NA
Genomic position
NA
95650
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments72.73%HighDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments90.02%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.74%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments87.70%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.08%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.57%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhB 836 CDS NA
TAIR10
NA NA
Genomic position
NA
95644
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products40.00%MediumNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products10.00%PoorNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.44%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments85.59%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments78.06%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments73.46%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments65.45%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 872 CDS NA
TAIR10
NA NA
Genomic position
NA
95608
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products85.00%HighNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.68%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments81.52%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments82.91%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments77.73%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments87.12%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 2 CDS NA
TAIR10
NA NA
Genomic position
NA
117166
C-to-U ACG=>AUG
NA=>NA
T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products45.00%MediumNone22411807
NAWTWild TypeWild TypeNo mutantNormalNANANANANANA30471730
Columbia (Col-0)ProMORF2: MORF2-4xMYCArabidopsis Col-0 transgenic plants expressing MORF2-4xMYC.To obtain MYC-tagged transgenic plants for MORF2 analysis, constructs expressing genes encoding MORF2-4xMYC fusion proteins, driven by the corresponding native promoters were transformed into wild-typNANormalNANANANANANA30471730
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments22.83%LowNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments29.07%LowNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments27.87%LowNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments21.10%LowNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments12.87%PoorNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 383 CDS NA
TAIR10
NA NA
Genomic position
NA
116785
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments74.22%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments56.57%MediumNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments53.85%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments69.07%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.70%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 674 CDS NA
TAIR10
NA NA
Genomic position
NA
116494
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products80.00%HighNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments74.32%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments62.50%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments58.72%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments79.79%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments80.14%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhD 878 CDS NA
TAIR10
NA NA
Genomic position
NA
116290
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products80.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products78.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products20.00%LowNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments62.91%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments53.91%MediumNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments73.25%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments69.09%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments74.32%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 887 CDS NA
TAIR10
NA NA
Genomic position
NA
116281
C-to-U CCC=>CUC
NA=>NA
P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products5.00%PoorDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments69.27%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments64.60%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments77.42%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments72.90%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments78.24%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhF 290 CDS NA
TAIR10
NA NA
Genomic position
NA
112235
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products10.00%PoorNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments4.70%PoorNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments4.85%PoorNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments4.00%PoorNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments7.52%PoorNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments12.38%PoorNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhG 50 CDS NA
TAIR10
NA NA
Genomic position
NA
118858
C-to-U UCC=>UUC
NA=>NA
S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products40.00%MediumDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments70.18%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments72.52%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments75.86%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments60.00%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments73.42%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast petL 5 CDS NA
TAIR10
NA NA
Genomic position
NA
65716
C-to-U CCU=>CUU
NA=>NA
P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products65.00%HighDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments43.64%MediumNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments26.00%LowNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments42.25%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments53.57%MediumNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments71.43%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast psbE 214 CDS NA
TAIR10
NA NA
Genomic position
NA
64109
C-to-U CCU=>UCU
NA=>NA
P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products30.00%LowDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments99.42%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments99.50%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments99.26%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments97.73%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.45%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast psbF 65 CDS NA NA NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast psbF 77 CDS TAIR10 NA Genomic position 63985 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.17%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments98.12%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments99.26%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.04%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.63%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
psbZ 50 CDS NA
TAIR10
NA NA
Genomic position
NA
35800
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products92.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products72.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products15.00%PoorNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments80.28%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments76.63%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments76.48%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments76.39%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments66.71%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rpl23 89 CDS NA
TAIR10
NA NA
Genomic position
NA
86055
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products70.00%HighNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments91.97%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments92.88%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments91.96%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.85%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.40%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rpoA 200 CDS NA
TAIR10
NA NA
Genomic position
NA
78691
C-to-U UCU=>UUU
NA=>NA
S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products80.00%HighIncreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products50.00%MediumNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments87.75%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments89.06%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.41%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.18%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments86.94%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
rpoB 2432 CDS NA
TAIR10
NA NA
Genomic position
NA
23898
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products80.00%HighDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products90.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products65.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products55.00%MediumNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments91.91%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.13%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments85.14%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.56%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.90%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
rpoB 338 CDS NA
TAIR10
NA NA
Genomic position
NA
25992
C-to-U UCU=>UUU
NA=>NA
S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products70.00%HighDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products85.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products55.00%MediumNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments84.82%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.50%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.22%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments82.83%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.85%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
rpoB 551 CDS NA
TAIR10
NA NA
Genomic position
NA
25779
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products80.00%HighDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products85.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products25.00%LowNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments94.25%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments92.11%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments90.74%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.36%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.45%HighNone37056496
Arabidopsis thaliana AT2G33430 Plastid
Chloroplast
rpoC1 488 CDS NA
TAIR10
NA NA
Genomic position
NA
21806
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products25.00%LowNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products30.00%LowNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products40.00%MediumNA30988197
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments33.33%LowNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments55.26%MediumNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments35.56%LowNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments35.48%LowNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments32.61%LowNone37056496
Arabidopsis thaliana AT2G33430 Plastid rps12 58 CDS NA NA NA NA C-to-U CAA/CAG=>UAA/UAG G=>Stop codon Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNULLNASeedlingNADirect Sequencing of PCR Products35.00%LowNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products50.00%MediumNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNull alleleNASeedlingNADirect Sequencing of PCR Products55.00%MediumNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNULLSeedling is pale and dieSeedlingNADirect Sequencing of PCR Products10.00%PoorNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast rps12 NA Intron NA
TAIR10
NA Genomic position 69553 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products15.00%PoorDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products20.00%LowNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments13.92%PoorNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments17.97%PoorNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments18.25%PoorNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments20.95%LowNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments15.50%PoorNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rps14 149 CDS NA
TAIR10
NA NA
Genomic position
NA
37092
C-to-U CCA=>CUA
NA=>NA
P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products95.00%HighNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments94.41%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.02%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.50%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments92.10%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments89.52%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rps14 80 CDS NA
TAIR10
NA NA
Genomic position
NA
37161
C-to-U UCA=>UUA
NA=>NA
S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments96.27%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments97.80%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments95.29%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments94.88%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.17%HighNone37056496
Last update: Feb 2026 (version 2.0)