MORF2 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MORF2
Synonym: DAL; DAL1; DIFFERENTIATION AND GREENING-LIKE; DIFFERENTIATION AND GREENING-LIKE 1; MULTIPLE ORGANELLAR RNA EDITING FACTOR 2; RIP2
Description: GUN1 plays a direct role in RNA editing by physically interacting with MULTIPLE ORGANELLAR RNA EDITING FACTOR 2 (MORF2); MORF2 overexpression causes widespread RNA-editing changes and a strong genomes uncoupled (gun) molecular phenotype similar to gun1 MORF2 further interacts with RNA-editing site-specificity factors: ORGANELLE TRANSCRIPT PROCESSING 81 (OTP81), ORGANELLE TRANSCRIPT PROCESSING 84 (OTP84), and YELLOW SEEDLINGS 1 (YS1); MORF2-MORF9 heterodimer is the core members of editosomes in chloroplasts, while they are not responsible for RNA editing site recognition; Reducing MORF2 by iCRISPRi stimulated the expression of stress responsive genes, triggered plastidial retrograde signaling, repressed ethylene signaling and skotomorphogenesis, and increased accumulation of hydrogen peroxide; MORF2 is an effective regulator involved in plastidial metabolic pathways whose reduction can readily activate multiple retrograde signaling molecules possibly involving reactive oxygen species to adjust plant growth
Protein Family: Multiple organellar RNA editing factor (MORF)
Subclass: NA
Physical Interaction: GUN1, MORF1; MORF9; MEF19; MEF21, MORF9; PDM1, OTP81; OTP84; YS1; GUN1
Construct Structure: NA
Gene ID & Species: AT2G33430 (Arabidopsis thaliana)
Edited Gene(s): ndhB    ndhD    psbE    psbF    atpF    clpP    accD    matK    ndhF    ndhG    petL    rpoB    rps14    rpoA    psbZ    rps12    rpl23    rpoC1
Editing Type(s): C-to-U (310)
Publication(s): [1] MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing, Front Plant Sci, 2023. [doi:10.3389/fpls.2023.1146922]
[2] GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling, PNAS, 2019. [doi:10.1073/pnas.1820426116]
[3] MORF2 tightly associates with MORF9 to regulate chloroplast RNA editing in Arabidopsis, Plant Sci, 2019. [doi:10.1016/j.plantsci.2018.10.020]
[4] Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants, PNAS, 2012. [doi:10.1073/pnas.1202452109]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G33430 Chloroplast accD 794 CDS TAIR10 NA Genomic region 57868 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments82.35%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments82.35%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments50.00%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments80.00%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.00%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast accD 794 CDS NA NA NA NA C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products4.00%PoorDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products89.00%HighNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast accD NA 3'UTR NA
TAIR10
NA Genomic position
Genomic region
58642 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products40.00%MediumIncreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products20.00%LowNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments50.00%MediumNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments72.22%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments50.00%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments54.17%MediumNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.12%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast atpF 92 CDS TAIR10 NA Genomic region 12707 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments88.60%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments89.62%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments84.00%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments86.79%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.20%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast atpF 92 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products10.00%PoorDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast clpP 559 CDS TAIR10 NA Genomic region 69942 C-to-U NA=>NA H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.00%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments76.41%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments74.94%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments78.52%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments73.46%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
clpP 559 CDS NA NA NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products60.00%HighDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products80.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products55.00%MediumNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products68.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products65.00%HighNone30988197
Arabidopsis thaliana AT2G33430 Chloroplast matK 706 CDS TAIR10 NA Genomic region 293167 C-to-U NA=>NA H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments69.70%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments60.10%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments65.77%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments77.03%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments77.21%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast matK 706 CDS NA NA NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products45.00%MediumDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products70.00%HighNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 1255 CDS TAIR10 NA Genomic region 95225 C-to-U NA=>NA H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments98.29%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.75%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.38%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments97.62%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.50%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 1255 CDS NA
TAIR10
NA NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products10.00%PoorDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments90.00%HighNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments50.00%MediumDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments90.20%HighSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments23.21%LowDecreased37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments91.23%HighSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments0.00%UneditedAbsent37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 1481 CDS TAIR10 NA Genomic region 94999 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.15%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments87.03%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.59%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.22%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.47%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 1481 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products10.00%PoorDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 149 CDS TAIR10 NA Genomic region 97016 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.02%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments79.03%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments77.61%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments79.53%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments62.31%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 149 CDS NA
TAIR10
NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments90.16%HighNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments30.16%LowDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments87.10%HighSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments73.68%HighDecreased37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments86.67%HighSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments0.00%UneditedAbsent37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 467 CDS TAIR10 NA Genomic region 96698 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments82.86%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments81.03%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.79%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments82.95%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments78.95%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhB 467 CDS NA
TAIR10
NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products5.00%PoorDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products20.00%LowNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatment. MORF2 is overexpressed and fused with 3xHA-3xFLAG tags in the wild-type Col6-3 background.NASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products15.00%PoorNA30988197
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments92.16%HighNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments14.55%PoorDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments87.27%HighSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments90.91%HighSimilar37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments84.91%HighSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments0.00%UneditedAbsent37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 586 CDS TAIR10 NA Genomic region 96579 C-to-U NA=>NA H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments89.32%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.54%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments83.03%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments88.70%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments87.36%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 586 CDS NA
TAIR10
NA NA NA C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products20.00%LowDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments13.73%PoorDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
SALK_094930morf2-2T-DNA insertionT-DNA insertion in an intronHomozygousRetarded embryo development and disrupted embryo structures that led to varied seedling morphology upon germinationSeedling7-d-oldSequencing amplified PCR fragments6.74%PoorDecreased37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 746 CDS TAIR10 NA Genomic region 96419 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.09%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments88.72%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments85.71%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments88.85%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments85.39%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 746 CDS NA NA NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 830 CDS TAIR10 NA Genomic region 95650 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments90.02%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.74%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments87.70%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.08%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.57%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 830 CDS NA
TAIR10
NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Columbia (Col-0)WT + Dex (5 μM)ControlControlNo mutantNormalSeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteNone37056496
Columbia (Col-0)P1-12 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments72.73%HighDecreased37056496
Columbia (Col-0)P1-10 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-54 + Dex (5 μM)Treated with DexTreated with 5 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Columbia (Col-0)P1-12 + Dex (0.25 μM)Treated with DexTreated with 0.25 μM DexNANASeedling7-d-oldSequencing amplified PCR fragments100.00%CompleteSimilar37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 836 CDS TAIR10 NA Genomic region 95644 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.44%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments85.59%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments78.06%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments73.46%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments65.45%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhB 836 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products40.00%MediumNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products10.00%PoorNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 872 CDS TAIR10 NA Genomic region 95608 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.68%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments81.52%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments82.91%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments77.73%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments87.12%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhB 872 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products85.00%HighNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 2 CDS TAIR10 NA Genomic region 117166 C-to-U NA=>NA T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments22.83%LowNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments29.07%LowNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments27.87%LowNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments21.10%LowNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments12.87%PoorNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 2 CDS NA NA NA NA C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products45.00%MediumNone22411807
NAWTWild TypeWild TypeNo mutantNormalNANANANANANA30471730
Columbia (Col-0)ProMORF2: MORF2-4xMYCArabidopsis Col-0 transgenic plants expressing MORF2-4xMYC.To obtain MYC-tagged transgenic plants for MORF2 analysis, constructs expressing genes encoding MORF2-4xMYC fusion proteins, driven by the corresponding native promoters were transformed into wild-typNANormalNANANANANANA30471730
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 383 CDS TAIR10 NA Genomic region 116785 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments74.22%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments56.57%MediumNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments53.85%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments69.07%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments83.70%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 383 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 674 CDS TAIR10 NA Genomic region 116494 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments74.32%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments62.50%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments58.72%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments79.79%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments80.14%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 674 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products80.00%HighNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 878 CDS TAIR10 NA Genomic region 116290 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments62.91%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments53.91%MediumNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments73.25%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments69.09%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments74.32%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhD 878 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products80.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products78.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products20.00%LowNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 887 CDS TAIR10 NA Genomic region 116281 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments69.27%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments64.60%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments77.42%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments72.90%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments78.24%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhD 887 CDS NA NA NA NA C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products5.00%PoorDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast ndhF 290 CDS TAIR10 NA Genomic region 112235 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments4.70%PoorNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments4.85%PoorNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments4.00%PoorNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments7.52%PoorNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments12.38%PoorNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
ndhF 290 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products10.00%PoorNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast ndhG 50 CDS TAIR10 NA Genomic region 118858 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments70.18%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments72.52%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments75.86%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments60.00%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments73.42%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast ndhG 50 CDS NA NA NA NA C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products40.00%MediumDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast petL 5 CDS TAIR10 NA Genomic region 65716 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments43.64%MediumNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments26.00%LowNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments42.25%MediumNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments53.57%MediumNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments71.43%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast petL 5 CDS NA NA NA NA C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products65.00%HighDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast psbE 214 CDS TAIR10 NA Genomic region 64109 C-to-U NA=>NA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments99.42%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments99.50%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments99.26%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments97.73%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.45%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast psbE 214 CDS NA NA NA NA C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products30.00%LowDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast psbF 65 CDS NA NA NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast psbF 77 CDS TAIR10 NA Genomic region 63985 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.17%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments98.12%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments99.26%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.04%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.63%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast psbZ 50 CDS TAIR10 NA Genomic region 35800 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments80.28%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments76.63%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments76.48%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments76.39%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments66.71%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
psbZ 50 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products92.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products72.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products15.00%PoorNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast rpl23 89 CDS TAIR10 NA Genomic region 86055 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments91.97%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments92.88%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments91.96%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.85%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.40%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rpl23 89 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products70.00%HighNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast rpoA 200 CDS TAIR10 NA Genomic region 78691 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments87.75%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments89.06%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments86.41%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.18%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments86.94%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rpoA 200 CDS NA NA NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products80.00%HighIncreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products50.00%MediumNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast rpoB 2432 CDS TAIR10 NA Genomic region 23898 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments91.91%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.13%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments85.14%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.56%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.90%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
rpoB 2432 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products80.00%HighDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products90.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products65.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products55.00%MediumNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast rpoB 338 CDS TAIR10 NA Genomic region 25992 C-to-U NA=>NA S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments84.82%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments84.50%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments79.22%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments82.83%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments93.85%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
rpoB 338 CDS NA NA NA NA C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products70.00%HighDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products85.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products75.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products55.00%MediumNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast rpoB 551 CDS TAIR10 NA Genomic region 25779 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments94.25%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments92.11%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments90.74%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments91.36%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.45%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast
Plastid
rpoB 551 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products80.00%HighDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products90.00%HighNone22411807
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products85.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products70.00%HighNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products25.00%LowNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast rpoC1 488 CDS TAIR10 NA Genomic region 21806 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments33.33%LowNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments55.26%MediumNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments35.56%LowNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments35.48%LowNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments32.61%LowNone37056496
Arabidopsis thaliana AT2G33430 Plastid rpoC1 488 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products60.00%HighNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products25.00%LowNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products30.00%LowNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products40.00%MediumNA30988197
Arabidopsis thaliana AT2G33430 Plastid rps12 58 CDS NA NA NA NA C-to-U CAA/CAG=>UAA/UAG Q=>Stop codon Nonsense
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col6-3Col6-3NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products35.00%LowNone30988197
Col6-3gun1-9NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products50.00%MediumNone30988197
Col6-3gun1-8NF treatmentA bleaching herbicide norflurazon (NF) treatmentNANASeedlingNADirect Sequencing of PCR Products55.00%MediumNone30988197
Col6-3MORF2OXNF treatment; OverexpressionA bleaching herbicide norflurazon (NF) treatmentNASeedling is pale and dieSeedlingNADirect Sequencing of PCR Products10.00%PoorNA30988197
Arabidopsis thaliana AT2G33430 Chloroplast rps12 NA Intron NA
TAIR10
NA Genomic position
Genomic region
69553 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products15.00%PoorDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products20.00%LowNone22411807
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments13.92%PoorNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments17.97%PoorNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments18.25%PoorNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments20.95%LowNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments15.50%PoorNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rps14 149 CDS TAIR10 NA Genomic region 37092 C-to-U NA=>NA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments94.41%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments95.02%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments93.50%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments92.10%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments89.52%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rps14 149 CDS NA NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products50.00%MediumDecreased22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products95.00%HighNone22411807
Arabidopsis thaliana AT2G33430 Chloroplast rps14 80 CDS TAIR10 NA Genomic region 37161 C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)KRAB (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments96.27%HighNone37056496
Columbia (Col-0)KRAB (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments97.80%HighNone37056496
Columbia (Col-0)P1-12 (+DMSO)Treated with DMSONANANASeedling8-d-oldSequencing amplified PCR fragments95.29%HighNone37056496
Columbia (Col-0)P1-12 (+0.025 mM Dex)Treated with DexTreated with 0.025 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments94.88%HighNone37056496
Columbia (Col-0)P1-12 (+0.25 mM Dex)Treated with DexTreated with 0.25 mM DexNANASeedling8-d-oldSequencing amplified PCR fragments90.17%HighNone37056496
Arabidopsis thaliana AT2G33430 Chloroplast rps14 80 CDS NA NA NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_094930Cmorf2-1T-DNA InsertionNAHomozygousStay whiteLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22411807
ColWTControlControlNo mutantNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22411807
Last update: Sep 2026 (version 2.0)