MORF2 - Plant Editosome Database

Summary

Editing Factor:	MORF2
Synonym:	DAL, DAL1, DIFFERENTIATION AND GREENING-LIKE, DIFFERENTIATION AND GREENING-LIKE 1, MULTIPLE ORGANELLAR RNA EDITING FACTOR 2
Description:	Encodes an editing factor involved at almost all RNA editing sites in chloroplasts.
Protein Family:	MORF/RIP
Subclass:	NA
Construct Structure:	NA
Gene ID & Species:	AT2G33430 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD psbE psbF atpF clpP accD matK ndhF ndhG petL rpoB rps14 rpoA psbZ rps12 rpl23 rpoC1
Editing Type(s):	C-to-U (109)
Publication(s):	[1] GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling, PNAS, 2019. [PMID=30988197] [2] Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants, PNAS, 2012. [PMID=22411807]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G33430

Chloroplast

accD

794

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	4.00%	Poor	Decreased	22411807
Col	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Direct Sequencing of PCR Products	89.00%	High	None	22411807

Arabidopsis thaliana

AT2G33430

Chloroplast

accD

58642

3'UTR

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	40.00%	Medium	Increased	22411807
Col	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Direct Sequencing of PCR Products	20.00%	Low	None	22411807

Arabidopsis thaliana

AT2G33430

Chloroplast

atpF

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	10.00%	Poor	Decreased	22411807
Col	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22411807

Arabidopsis thaliana

AT2G33430

Chloroplast
Plastid

clpP

559

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_094930C	morf2-1	T-DNA Insertion	NA	Homozygous	Stay white	Leaf	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	22411807
Col	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Direct Sequencing of PCR Products	80.00%	High	None	22411807
Col6-3	Col6-3	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedlings	NA	Direct Sequencing of PCR Products	55.00%	Medium	None	30988197
Col6-3	gun1-9	NF treatment	A bleaching herbicide norflurazon (NF) treatment	Null allele	NA	Seedlings	NA	Direct Sequencing of PCR Products	68.00%	High	None	30988197
Col6-3	gun1-8	NF treatment	A bleaching herbicide norflurazon (NF) treatment