| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
accD
|
794 |
CDS |
C-to-U |
UCG=>UUG
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 4.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 89.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 80.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 89.89% | High | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT | mock | Sugar starvation | No mutant | Normal | Root | NA | Sequencing PCR products | 100.00% | Complete | None | 38357785 | | Columbia (Col-0) | WT | Sucrose exposure | 2% sucrose exposure | No mutant | NA | Root | NA | Sequencing PCR products | 100.00% | Complete | None | 38357785 | | Columbia (Col-0) | morf9 | mock | Mutant; Sugar starvation | Homozygous | A dwarfed seedling characterized by pale yellow leaves and highly reduced root length, diminished photosynthetic fluorescence intensity, derangements in thylakoid membrane structure in the chloroplasts, and decreased PSII chemical quantum efficiency (Fv/Fm) and NDH activity relative to wild type plants (WT); Furthermore, sucrose content was significantly decreased in 28-day-old morf9 plants compared with WT when grown in absence of glucose in the medium, although glucose content remained stable in the morf9 mutant leaves; Roots of morf9 mutant plants were significantly shorter and associated with a slower root elongation rate relative to WT in sugar deficiency medium; Propidium iodide staining revealed smaller root meristems (i.e., fewer epidermal cells between the QC and the meristem–elongation zone boundary), and shorter mature epidermal cells in morf9 mutants compared with WT plants | Leaf; Root | NA | Sequencing PCR products | 90.00% | High | Decreased | 38357785 | | Columbia (Col-0) | morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | Homozygous | NA | Leaf; Root | NA | Sequencing PCR products | 90.00% | High | Decreased | 38357785 | | Columbia (Col-0) | gin2 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Similar | 38357785 | | Columbia (Col-0) | gin2 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Similar | 38357785 | | Columbia (Col-0) | snrk1 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 50.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | snrk1 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Similar | 38357785 | | Columbia (Col-0) | gin2morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Similar | 38357785 | | Columbia (Col-0) | gin2morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Similar | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | mock | Overexpressing HXK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | Sucrose exposure | Overexpressing HXK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | snrk1morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 50.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | snrk1morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Similar | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | mock | Overexpressing SnRK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | Sucrose exposure | Overexpressing SnRK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
accD
|
NA |
3'UTR |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 40.00% | Medium | Increased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 20.00% | Low | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
atpF
|
92 |
CDS |
C-to-U |
CCA=>CUA
NA=>NA |
P=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
clpP
|
559 |
CDS |
C-to-U |
CAU=>UAU |
H=>Y |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 40.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 80.00% | High | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Plastid |
clpP1
|
559 |
CDS |
C-to-U |
NA=>NA |
H=>Y |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 45.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 79.90% | High | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
matK
|
706 |
CDS |
C-to-U |
CAU=>UAU
NA=>NA |
H=>Y |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 70.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 80.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 60.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 80.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 80.44% | High | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 80.41% | High | None | 31546885 | | Columbia (Col-0) | WT | mock | Sugar starvation | No mutant | Normal | Root | NA | Sequencing PCR products | 90.00% | High | None | 38357785 | | Columbia (Col-0) | WT | Sucrose exposure | 2% sucrose exposure | No mutant | NA | Root | NA | Sequencing PCR products | 90.00% | High | None | 38357785 | | Columbia (Col-0) | morf9 | mock | Mutant; Sugar starvation | Homozygous | A dwarfed seedling characterized by pale yellow leaves and highly reduced root length, diminished photosynthetic fluorescence intensity, derangements in thylakoid membrane structure in the chloroplasts, and decreased PSII chemical quantum efficiency (Fv/Fm) and NDH activity relative to wild type plants (WT); Furthermore, sucrose content was significantly decreased in 28-day-old morf9 plants compared with WT when grown in absence of glucose in the medium, although glucose content remained stable in the morf9 mutant leaves; Roots of morf9 mutant plants were significantly shorter and associated with a slower root elongation rate relative to WT in sugar deficiency medium; Propidium iodide staining revealed smaller root meristems (i.e., fewer epidermal cells between the QC and the meristem–elongation zone boundary), and shorter mature epidermal cells in morf9 mutants compared with WT plants | Leaf; Root | NA | Sequencing PCR products | 70.00% | High | Decreased | 38357785 | | Columbia (Col-0) | morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | Homozygous | NA | Leaf; Root | NA | Sequencing PCR products | 55.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | gin2 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 70.00% | High | Decreased | 38357785 | | Columbia (Col-0) | gin2 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Similar | 38357785 | | Columbia (Col-0) | snrk1 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 60.00% | High | Decreased | 38357785 | | Columbia (Col-0) | snrk1 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 90.00% | High | Similar | 38357785 | | Columbia (Col-0) | gin2morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 70.00% | High | Decreased | 38357785 | | Columbia (Col-0) | gin2morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Similar | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | mock | Overexpressing HXK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Restored | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | Sucrose exposure | Overexpressing HXK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | snrk1morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 30.00% | Low | Decreased | 38357785 | | Columbia (Col-0) | snrk1morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 90.00% | High | Similar | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | mock | Overexpressing SnRK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Restored | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | Sucrose exposure | Overexpressing SnRK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
ndhB
|
1255 |
CDS |
C-to-U |
CAU=>UAU |
H=>Y |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 15.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
ndhB
|
1481 |
CDS |
C-to-U |
CCA=>CUA |
P=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
ndhB
|
149 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 90.00% | High | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhB
|
467 |
CDS |
C-to-U |
CCA=>CUA
NA=>NA |
P=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 15.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 90.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 20.00% | Low | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Root | 21 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhB
|
586 |
CDS |
C-to-U |
CAU=>UAU
NA=>NA |
H=>Y |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Root | 21 days | Bulk-cDNA sequencing | 80.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhB
|
746 |
CDS |
C-to-U |
UCU=>UUU
NA=>NA |
S=>F |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Root | 21 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhB
|
830 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhB
|
836 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 35.00% | Low | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 30.00% | Low | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Root | 21 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhB
|
872 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 85.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 80.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Root | 21 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
ndhD
|
2 |
CDS |
C-to-U |
ACG=>AUG |
T=>M |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 45.00% | Medium | None | 22411807 | | NA | WT | Wild Type | Wild Type | No mutant | Normal | NA | NA | NA | NA | NA | NA | 30471730 | | Salk_013483 | morf9 | A T-DNA insertion | The T-DNA was inserted in the 5′-untranslated region of the MORF9 gene. | Homozygous | A dwarf phenotype and green leaves with an albino margin | NA | NA | NA | NA | NA | NA | 30471730 | | Columbia (Col-0) | ProMORF9: MORF9-4xMYC | Complementation | We then created transgenic complemented morf9 plants expressing a gene encoding a 4xMYC-tagged MORF9 fusion protein (MORF9-4xMYC) under the control of the native MORF9 promoter. | Homozygous | Normal | NA | NA | NA | NA | NA | NA | 30471730 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhD
|
383 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 40.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 90.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 60.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT | mock | Sugar starvation | No mutant | Normal | Root | NA | Sequencing PCR products | 100.00% | Complete | None | 38357785 | | Columbia (Col-0) | WT | Sucrose exposure | 2% sucrose exposure | No mutant | NA | Root | NA | Sequencing PCR products | 90.00% | High | None | 38357785 | | Columbia (Col-0) | morf9 | mock | Mutant; Sugar starvation | Homozygous | A dwarfed seedling characterized by pale yellow leaves and highly reduced root length, diminished photosynthetic fluorescence intensity, derangements in thylakoid membrane structure in the chloroplasts, and decreased PSII chemical quantum efficiency (Fv/Fm) and NDH activity relative to wild type plants (WT); Furthermore, sucrose content was significantly decreased in 28-day-old morf9 plants compared with WT when grown in absence of glucose in the medium, although glucose content remained stable in the morf9 mutant leaves; Roots of morf9 mutant plants were significantly shorter and associated with a slower root elongation rate relative to WT in sugar deficiency medium; Propidium iodide staining revealed smaller root meristems (i.e., fewer epidermal cells between the QC and the meristem–elongation zone boundary), and shorter mature epidermal cells in morf9 mutants compared with WT plants | Leaf; Root | NA | Sequencing PCR products | 60.00% | High | Decreased | 38357785 | | Columbia (Col-0) | morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | Homozygous | NA | Leaf; Root | NA | Sequencing PCR products | 50.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | gin2 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 60.00% | High | Decreased | 38357785 | | Columbia (Col-0) | gin2 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Similar | 38357785 | | Columbia (Col-0) | snrk1 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Similar | 38357785 | | Columbia (Col-0) | snrk1 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 60.00% | High | Decreased | 38357785 | | Columbia (Col-0) | gin2morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 60.00% | High | Decreased | 38357785 | | Columbia (Col-0) | gin2morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 65.00% | High | Decreased | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | mock | Overexpressing HXK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | Sucrose exposure | Overexpressing HXK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | snrk1morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 60.00% | High | Decreased | 38357785 | | Columbia (Col-0) | snrk1morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 95.00% | High | Similar | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | mock | Overexpressing SnRK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | Sucrose exposure | Overexpressing SnRK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhD
|
674 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 80.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 60.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Root | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Increased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 90.06% | High | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 89.48% | High | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhD
|
878 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 80.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 60.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 60.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Root | 21 days | Bulk-cDNA sequencing | 60.00% | High | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhD
|
887 |
CDS |
C-to-U |
CCC=>CUC
NA=>NA |
P=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 60.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 50.00% | Medium | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 50.00% | Medium | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 10.00% | Poor | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf14) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf14 mutant. | Root | 21 days | Bulk-cDNA sequencing | 10.00% | Poor | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhF
|
290 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 40.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 20.00% | Low | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Root | 21 days | Bulk-cDNA sequencing | 20.00% | Low | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 90.02% | High | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT | mock | Sugar starvation | No mutant | Normal | Root | NA | Sequencing PCR products | 100.00% | Complete | None | 38357785 | | Columbia (Col-0) | WT | Sucrose exposure | 2% sucrose exposure | No mutant | NA | Root | NA | Sequencing PCR products | 95.00% | High | None | 38357785 | | Columbia (Col-0) | morf9 | mock | Mutant; Sugar starvation | Homozygous | A dwarfed seedling characterized by pale yellow leaves and highly reduced root length, diminished photosynthetic fluorescence intensity, derangements in thylakoid membrane structure in the chloroplasts, and decreased PSII chemical quantum efficiency (Fv/Fm) and NDH activity relative to wild type plants (WT); Furthermore, sucrose content was significantly decreased in 28-day-old morf9 plants compared with WT when grown in absence of glucose in the medium, although glucose content remained stable in the morf9 mutant leaves; Roots of morf9 mutant plants were significantly shorter and associated with a slower root elongation rate relative to WT in sugar deficiency medium; Propidium iodide staining revealed smaller root meristems (i.e., fewer epidermal cells between the QC and the meristem–elongation zone boundary), and shorter mature epidermal cells in morf9 mutants compared with WT plants | Leaf; Root | NA | Sequencing PCR products | 70.00% | High | Decreased | 38357785 | | Columbia (Col-0) | morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | Homozygous | NA | Leaf; Root | NA | Sequencing PCR products | 50.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | gin2 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 40.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | gin2 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 55.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | snrk1 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 70.00% | High | Decreased | 38357785 | | Columbia (Col-0) | snrk1 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 30.00% | Low | Decreased | 38357785 | | Columbia (Col-0) | gin2morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 20.00% | Low | Decreased | 38357785 | | Columbia (Col-0) | gin2morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 40.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | mock | Overexpressing HXK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | oeHXK1morf9 | Sucrose exposure | Overexpressing HXK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | snrk1morf9 | mock | Mutant; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 50.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | snrk1morf9 | Sucrose exposure | Mutant; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 50.00% | Medium | Decreased | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | mock | Overexpressing SnRK1; Sugar starvation | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 | | Columbia (Col-0) | oesnRK1morf9 | Sucrose exposure | Overexpressing SnRK1; 2% sucrose exposure | NA | NA | Leaf | NA | Sequencing PCR products | 100.00% | Complete | Restored | 38357785 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
ndhG
|
50 |
CDS |
C-to-U |
UCC=>UUC
NA=>NA |
S=>F
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
petL
|
5 |
CDS |
C-to-U |
CCU=>CUU |
P=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Plastid |
psbE
|
214 |
CDS |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
psbF
|
65 |
CDS |
C-to-U |
NA=>NA |
NA=>NA
S=>F |
NA
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 70.00% | High | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf13) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf13 mutant. | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 90.30% | High | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
psbZ
|
50 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 30.00% | Low | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 60.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
rpl23
|
89 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 70.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 60.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Root | 21 days | Bulk-cDNA sequencing | 80.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 80.37% | High | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 90.16% | High | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
rpoA
|
200 |
CDS |
C-to-U |
UCU=>UUU
NA=>NA |
S=>F |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 50.00% | Medium | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf10) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf10 mutant. | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | Similar | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 80.05% | High | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 70.78% | High | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
rpoB
|
2432 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 80.00% | High | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
rpoB
|
338 |
CDS |
C-to-U |
UCU=>UUU |
S=>F |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 70.00% | High | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 90.00% | High | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
rpoB
|
551 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 60.00% | High | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 90.00% | High | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Plastid |
rpoC1
|
488 |
CDS |
C-to-U |
NA=>NA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 20.00% | Low | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 20.00% | Low | Similar | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 50.00% | Medium | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 30.00% | Low | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 50.00% | Medium | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf9) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf9 mutant. | Root | 21 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 20.07% | Low | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 20.21% | Low | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast |
rps12
|
NA |
Intron |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 20.00% | Low | None | 22411807 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
rps14
|
149 |
CDS |
C-to-U |
CCA=>CUA
NA=>NA |
P=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 95.00% | High | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 50.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 30.00% | Low | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 80.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf12) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf12 mutant. | Root | 21 days | Bulk-cDNA sequencing | 40.00% | Medium | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 40.24% | Medium | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 |
|
| Arabidopsis thaliana |
AT1G11430 |
Chloroplast
Plastid |
rps14
|
80 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| SALK_013483 (E) (R) | morf9-1 | T-DNA Insertion | NA | Homozygous | Defects in greening in light | Leaf | NA | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22411807 | | Col | WT | Control | Control | Control | Normal | Leaf | NA | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22411807 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 100.00% | Complete | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Leaf | 21 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Flower | 56 days | Bulk-cDNA sequencing | 80.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Flower | 56 days | Bulk-cDNA sequencing | 60.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT | Wild Type | Wild Type | No mutant | Normal | Root | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 | | Columbia (Col-0) | morf9 | A T-DNA insertion | T-DNA insertion lines SALK_013483 (morf11) were provided by NASC. | Homozygous | Phenotypically, morf9 seedlings grown in sugar-free 1/2 MS medium showed a stunted plant, short roots, and yellow leaves. These plants exhibited retarded growth with yellow variegated leaves after they were transferred to soil for 35 days. At 12 days after germination (dag), the average root length of the morf9 seedlings was one-sixth shorter than that of WT ones, whereas the average root length of plants overexpressing MORF9-GFP did not differ significantly from that of WT plants. Both yellowish rosette leaves and shortened roots during sugar-free germination were obvious in morf11 mutant. | Root | 21 days | Bulk-cDNA sequencing | 70.00% | High | Decreased | 31546885 | | Columbia (Col-0) | WT-leaf-12d | Wild Type | Wild Type | No mutant | Normal | Leaf | 12 days | Bulk-cDNA sequencing | 45.31% | Medium | None | 31546885 | | Columbia (Col-0) | WT-leaf-21d | Wild Type | Wild Type | No mutant | Normal | Leaf | 21 days | Bulk-cDNA sequencing | 90.00% | High | None | 31546885 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
atpA
|
1148 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhA
|
1070 |
CDS |
C-to-U |
UCC=>UUC |
S=>F |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 73.91% | High | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 20.00% | Low | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 17.86% | Poor | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhB
|
467 |
CDS |
C-to-U |
CCA=>CUA |
P=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 40.54% | Medium | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 32.43% | Low | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhB
|
586 |
CDS |
C-to-U |
CAU=>UAU |
H=>Y |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 20.69% | Low | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 28.57% | Low | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhB
|
611 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 30.77% | Low | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 17.95% | Poor | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhB
|
704 |
CDS |
C-to-U |
UCC=>UUC |
S=>F |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 91.67% | High | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 44.68% | Medium | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 32.50% | Low | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhB
|
737 |
CDS |
C-to-U |
CCA=>CUA |
P=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 62.22% | High | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 11.76% | Poor | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhB
|
830 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 32.00% | Low | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 83.67% | High | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhB
|
836 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 35.42% | Low | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 77.78% | High | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhD
|
878 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 80.00% | High | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ndhG
|
10 |
CDS |
C-to-U |
CUU=>UUU |
L=>F |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 57.14% | Medium | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 55.56% | Medium | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
rpl2
|
2 |
CDS |
C-to-U |
ACG=>AUG |
T=>M |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 50.00% | Medium | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
rpoB
|
467 |
CDS |
C-to-U |
UCG=>UUG |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
rpoB
|
545 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 80.77% | High | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 16.67% | Poor | Decreased | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 19.61% | Poor | Decreased | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
rpoC2
|
4106 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
rps14
|
80 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
rps8
|
182 |
CDS |
C-to-U |
UCA=>UUA |
S=>L |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|
| Oryza sativa |
LOC_Os08g04450 |
Chloroplast |
ycf3
|
185 |
CDS |
C-to-U |
ACG=>AUG |
T=>M |
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Leaf | NA | Sequencing RT-PCR products | 100.00% | Complete | None | 33902846 | | Nipponbare | osmorf9-1 | Deletion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 | | Nipponbare | osmorf9-2 | Insertion | Sequence analysis showed that the osmorf9-1 and osmorf9-2 mutants had a 1 bp deletion and 2 bp insertion, respectively, at the target site. | Knockout | Phenotypic analysis showed that both mutants exhibited albino phenotypes and died after about one month. Thereafter, the study measured the levels of chlorophyll a (Chl a) and b (Chl b). The findings revealed that there was a significant decrease in the levels of both Chl a and Chl b in the osmorf9 mutants. Furthermore, the Fv/Fm ratio was also significantly decreased in the osmorf9-1 and osmorf9-2 mutants compared to the WT. In addition, the ultrastructure of chloroplast in WT, osmorf9-1 and osmorf9-2 mutant plants were observed through TEM. When compared with WT, abnormal chloroplasts which lacked stacked grana and thylakoid membranes were observed in the leaves of the osmorf9-1 and osmorf9-2 mutants. | Leaf | NA | Sequencing RT-PCR products | 0.00% | Unedited | Absent | 33902846 |
|