ORRM1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: ORRM1
Synonym: ORGANELLE RRM PROTEIN 1
Description: Encodes a unique protein that is both a member of the RIP (RNA-editing interacting protein) family and the RNA recognition motif (RRM)-containing family.
Protein Family: ORRM
Subclass: NA,ORRM
Construct Structure: RRM, PTP-RIP-ORRM
Gene ID & Species: AT3G20930 (Arabidopsis thaliana)
GRMZM5G899787 (Zea mays subsp. mays)
GRMZM5G899787 (Zea mays var. Sugar Buns)
Edited Gene(s): accD    clpP    matK    ndhB    ndhD    ndhG    rpoA    rpoB    rps12    rps14    atpA    ndhA    petB    rpl2    rpl20    rpoC2    rps8    ycf3    psbF    atpF    ndhF    petL    psbE    psbZ    rpl23    rpoC1
Editing Type(s): C-to-U (178)
Publication(s): [1] Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in the Arabidopsis thaliana plastid, Plant Direct, 2020. [PMID=32259001]
[2] Stable native RIP9 complexes associate with C-to-U RNA editing activity, PPRs, RIPs, OZ1, ORRM1, and ISE2, The Plant Journal, 2019. [PMID=31077462]
[3] An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize, PNAS, 2013. [PMID=23487777]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At3g20930 Plastid accD 794 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
accD 1568 3'UTR
CDS
C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq81.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq67.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq1.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq4.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid atpF 92 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
clpP 559 CDS C-to-U CAU=>UAU
NA=>NA
H=>Y
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq90.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq24.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq40.00%MediumDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing33.33%LowDecreased32259001
Arabidopsis thaliana At3g20930 Plastid matK 640 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast matK 706 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq89.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq7.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq1.00%PoorDecreased23487777
Arabidopsis thaliana At3g20930 Plastid ndhB 149 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 467 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq98.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq91.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq5.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq4.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 586 CDS C-to-U CAU=>UAU
NA=>NA
H=>Y
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq98.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq92.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq5.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq6.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 746 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq98.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq94.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq46.00%MediumDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq49.00%MediumDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing50.00%MediumDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 830 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq94.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq84.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq28.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq22.00%LowDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing18.75%PoorDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 836 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq88.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq73.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq4.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq8.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 872 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq95.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq1.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq1.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 1255 CDS C-to-U CAU=>UAU
NA=>NA
H=>Y
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq100.00%CompleteNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq95.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq40.00%MediumDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq42.00%MediumDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing36.84%MediumDecreased32259001
Arabidopsis thaliana At3g20930 Plastid ndhB 1481 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 2 CDS C-to-U ACG=>AUG
NA=>NA
T=>M
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq46.00%MediumNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq54.00%MediumNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq21.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq31.00%LowDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 674 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq96.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq88.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq2.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq2.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 878 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq87.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq86.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq2.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq3.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 887 CDS C-to-U CCC=>CUC
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq88.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq3.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq3.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid ndhF 290 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhG 50 CDS C-to-U UCC=>UUC
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq80.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq77.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq5.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq7.00%PoorDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid petL 5 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing77.78%HighSimilar32259001
Arabidopsis thaliana At3g20930 Plastid psbE 214 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana At3g20930 Plastid psbF 77 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana At3g20930 Plastid psbZ 50 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing94.44%HighSimilar32259001
Arabidopsis thaliana At3g20930 Plastid rpl23 89 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing14.29%PoorSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoA 200 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq81.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq40.00%MediumNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq22.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq22.00%LowDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing44.44%MediumDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoB 338 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq93.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq83.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq80.00%HighDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq77.00%HighDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing81.25%HighDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoB 551 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq96.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq83.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq25.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq22.00%LowDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing33.33%LowDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoB 2432 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq96.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq81.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq8.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq20.00%LowDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid rpoC1 488 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing30.43%LowSimilar32259001
Arabidopsis thaliana At3g20930 Plastid rps12 null Intronic C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast rps12 58 Intronic C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq40.00%MediumNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq33.00%LowNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq0.00%UneditedAbsent23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq0.00%UneditedAbsent23487777
Arabidopsis thaliana At3g20930 Plastid rps14 80 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing90.91%HighSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rps14 149 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAGene specific RNA-seq92.00%HighNone23487777
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNATotal plastid RNA-seq90.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq60.00%HighDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq75.00%HighDecreased23487777
Columbiaorrm1-1T‐DNA insertion lines orrm1‐1 (SALK_072648)NAHomozygousThe orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.Rosette leaf4-weekRT‐PCR and Sanger sequencing50.00%MediumAbsent32259001
Zea mays subsp. mays GRMZM5G899787 Chloroplast atpA 1148 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast matK 1258 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhA 50 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhA 1070 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhB 467 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%LowDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay31.00%LowDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAPoisoned Primer Extension (PPE) Assay97.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhB 586 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay7.00%PoorDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAPoisoned Primer Extension (PPE) Assay98.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhB 737 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%LowDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhB 830 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay65.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAPoisoned Primer Extension (PPE) Assay99.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhD 878 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay29.00%LowDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAPoisoned Primer Extension (PPE) Assay96.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ndhG 347 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast petB 668 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rpl2 2 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products70.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rpl20 308 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rpoB 467 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rpoB 545 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay14.00%PoorDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAPoisoned Primer Extension (PPE) Assay89.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rpoB 560 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rpoB 617 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products40.00%MediumDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products80.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rpoC2 2774 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rps14 80 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay90.00%HighDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNAPoisoned Primer Extension (PPE) Assay97.00%HighNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast rps8 182 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products30.00%LowDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ycf3 44 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays subsp. mays GRMZM5G899787 Chloroplast ycf3 185 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays var. Sugar Buns GRMZM5G899787 Chloroplast atpA 1148 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf14–21 daysDirect Sequencing of PCR Products NALowNone31077462
Zea mays var. Sugar Buns GRMZM5G899787 Chloroplast ndhA 473 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf14–21 daysDirect Sequencing of PCR Products NALowNone31077462
Zea mays var. Sugar Buns GRMZM5G899787 Chloroplast rpl20 308 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf14–21 daysDirect Sequencing of PCR Products NALowNone31077462
Zea mays var. Sugar Buns GRMZM5G899787 Chloroplast rpoC2 2774 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf14–21 daysDirect Sequencing of PCR Products NAHighNone31077462
Last update: Jul 2021 (version 1.0)