ORRM1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

At3g20930

Plastid

accD

794

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

accD

1568

3'UTR
CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	81.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	67.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	1.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	4.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

At3g20930

Plastid

atpF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

clpP

559

CDS

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	90.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	24.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	40.00%	Medium	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	33.33%	Low	Decreased	32259001

Arabidopsis thaliana

At3g20930

Plastid

matK

640

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast

matK

706

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	89.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	7.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	1.00%	Poor	Decreased	23487777

Arabidopsis thaliana

At3g20930

Plastid

ndhB

149

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

467

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	98.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	91.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	5.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	4.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

586

CDS

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	98.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	92.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	5.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	6.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

746

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	98.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	94.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	46.00%	Medium	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	49.00%	Medium	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	50.00%	Medium	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

830

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	94.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	84.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	28.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	22.00%	Low	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	18.75%	Poor	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

836

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	88.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	73.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	4.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	8.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

872

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	95.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	1.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	1.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

1255

CDS

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	100.00%	Complete	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	95.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	40.00%	Medium	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	42.00%	Medium	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	36.84%	Medium	Decreased	32259001

Arabidopsis thaliana

At3g20930

Plastid

ndhB

1481

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

CDS

C-to-U

ACG=>AUG
NA=>NA

T=>M
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	46.00%	Medium	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	54.00%	Medium	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	21.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	31.00%	Low	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

At3g20930

Plastid

ndhD

383

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

674

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	96.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	88.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	2.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	2.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

878

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	87.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	86.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	2.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	3.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

887

CDS

C-to-U

CCC=>CUC
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	88.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	3.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	3.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

At3g20930

Plastid

ndhF

290

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhG

CDS

C-to-U

UCC=>UUC
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	80.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	77.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	5.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	7.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

At3g20930

Plastid

petL

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	77.78%	High	Similar	32259001

Arabidopsis thaliana

At3g20930

Plastid

psbE

214

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

At3g20930

Plastid

psbF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

At3g20930

Plastid

psbZ

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	94.44%	High	Similar	32259001

Arabidopsis thaliana

At3g20930

Plastid

rpl23

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	14.29%	Poor	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoA

200

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	81.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	40.00%	Medium	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	22.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	22.00%	Low	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	44.44%	Medium	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoB

338

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	93.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	83.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	80.00%	High	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	77.00%	High	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	81.25%	High	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoB

551

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	96.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	83.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	25.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	22.00%	Low	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	33.33%	Low	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoB

2432

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	96.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	81.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	8.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	20.00%	Low	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

At3g20930

Plastid

rpoC1

488

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	30.43%	Low	Similar	32259001

Arabidopsis thaliana

At3g20930

Plastid

rps12

null

Intronic

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast

rps12

Intronic

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	40.00%	Medium	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	33.00%	Low	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	0.00%	Unedited	Absent	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	0.00%	Unedited	Absent	23487777

Arabidopsis thaliana

At3g20930

Plastid

rps14

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	90.91%	High	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rps14

149

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Gene specific RNA-seq	92.00%	High	None	23487777
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Total plastid RNA-seq	90.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	60.00%	High	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	75.00%	High	Decreased	23487777
Columbia	orrm1-1	T‐DNA insertion lines orrm1‐1 (SALK_072648)	NA	Homozygous	The orrm1‐1 mutant was actually slightly bigger than the Columbia wild type.	Rosette leaf	4-week	RT‐PCR and Sanger sequencing	50.00%	Medium	Absent	32259001

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

atpA

1148

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

matK

1258

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhA

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhA

1070

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhB

467

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Low	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	31.00%	Low	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Poisoned Primer Extension (PPE) Assay	97.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhB

586

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	7.00%	Poor	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Poisoned Primer Extension (PPE) Assay	98.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhB

737

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Low	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhB

830

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	60.00%	High	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	65.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Poisoned Primer Extension (PPE) Assay	99.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhD

878

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	20.00%	Low	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	29.00%	Low	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Poisoned Primer Extension (PPE) Assay	96.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ndhG

347

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

petB

668

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rpl2

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	70.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rpl20

308

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	20.00%	Low	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rpoB

467

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	60.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rpoB

545

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	14.00%	Poor	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Poisoned Primer Extension (PPE) Assay	89.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rpoB

560

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	60.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rpoB

617

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	40.00%	Medium	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	80.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rpoC2

2774

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rps14

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	90.00%	High	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Poisoned Primer Extension (PPE) Assay	97.00%	High	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

rps8

182

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	30.00%	Low	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ycf3

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	20.00%	Low	Decreased	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays subsp. mays

GRMZM5G899787

Chloroplast

ycf3

185

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays var. Sugar Buns

GRMZM5G899787

Chloroplast

atpA

1148

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	14–21 days	Direct Sequencing of PCR Products	NA	Low	None	31077462

Zea mays var. Sugar Buns

GRMZM5G899787

Chloroplast

ndhA

473

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	14–21 days	Direct Sequencing of PCR Products	NA	Low	None	31077462

Zea mays var. Sugar Buns

GRMZM5G899787

Chloroplast

rpl20

308

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	14–21 days	Direct Sequencing of PCR Products	NA	Low	None	31077462

Zea mays var. Sugar Buns

GRMZM5G899787

Chloroplast

rpoC2

2774

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	14–21 days	Direct Sequencing of PCR Products	NA	High	None	31077462

Editing Factor:	ORRM1
Synonym:	ORGANELLE RRM PROTEIN 1
Description:	Encodes a unique protein that is both a member of the RIP (RNA-editing interacting protein) family and the RNA recognition motif (RRM)-containing family.
Protein Family:	ORRM
Subclass:	NA,ORRM
Construct Structure:	RRM, PTP-RIP-ORRM
Gene ID & Species:	AT3G20930 (Arabidopsis thaliana) GRMZM5G899787 (Zea mays subsp. mays) GRMZM5G899787 (Zea mays var. Sugar Buns)
Edited Gene(s):	accD clpP matK ndhB ndhD ndhG rpoA rpoB rps12 rps14 atpA ndhA petB rpl2 rpl20 rpoC2 rps8 ycf3 psbF atpF ndhF petL psbE psbZ rpl23 rpoC1
Editing Type(s):	C-to-U (178)
Publication(s):	[1] Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in the Arabidopsis thaliana plastid, Plant Direct, 2020. [PMID=32259001] [2] Stable native RIP9 complexes associate with C-to-U RNA editing activity, PPRs, RIPs, OZ1, ORRM1, and ISE2, The Plant Journal, 2019. [PMID=31077462] [3] An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize, PNAS, 2013. [PMID=23487777]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details