ORRM1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: ORRM1
Synonym: ORGANELLE RRM PROTEIN 1
Description: Encoding a unique protein that is both a member of the RIP (RNA-editing interacting protein) family and the RNA recognition motif (RRM)-containing family
Protein Family: ORRM
Subclass: NA
Construct Structure: NA, PTP-RIP-ORRM
Gene ID & Species: AT3G20930 (Arabidopsis thaliana)
GRMZM5G899787 (Zea mays)
Edited Gene(s): accD    clpP    matK    ndhB    ndhD    ndhG    rpoA    rpoB    rps12    rps14    atpA    ndhA    petB    rpl2    rpl20    rpoC2    rps8    ycf3    atpF    ndhF    petL    psbE    psbF    psbZ    rpl23    rpoC1
Editing Type(s): C-to-U (178)
Publication(s): [1] Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in the plastid., Plant direct, 2020. [PMID=32259001]
[2] Stable native RIP9 complexes associate with C-to-U RNA editing activity, PPRs, RIPs, OZ1, ORRM1 and ISE2., The Plant journal : for cell and molecular biology, 2019. [PMID=31077462]
[3] An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize., Proceedings of the National Academy of Sciences of the United States of America, 2013. [PMID=23487777]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At3g20930 Plastid accD 1568 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid accD 794 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast accD NA 3'UTR C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq81.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq67.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq1.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq4.00%PoorDecreased23487777
Arabidopsis thaliana At3g20930 Plastid atpF 92 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
clpP 559 CDS C-to-U CAU=>UAU
NA=>NA
H=>Y
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq90.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq24.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq40.00%MediumDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing33.33%LowDecreased32259001
Arabidopsis thaliana At3g20930 Plastid matK 640 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast matK 706 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq89.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq7.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq1.00%PoorDecreased23487777
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 1255 CDS C-to-U CAU=>UAU
NA=>NA
H=>Y
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq100.00%CompleteNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq95.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq40.00%MediumDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq42.00%MediumDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing36.84%LowDecreased32259001
Arabidopsis thaliana At3g20930 Plastid ndhB 1481 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana At3g20930 Plastid ndhB 149 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 467 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq98.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq91.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq5.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq4.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 586 CDS C-to-U CAU=>UAU
NA=>NA
H=>Y
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq98.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq92.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq5.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq6.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 746 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq98.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq94.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq46.00%MediumDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq49.00%MediumDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing50.00%MediumDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 830 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq94.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq84.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq28.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq22.00%LowDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing18.75%PoorDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 836 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq88.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq73.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq4.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq8.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhB 872 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq95.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq1.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq1.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 2 CDS C-to-U ACG=>AUG
NA=>NA
T=>M
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq46.00%MediumNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq54.00%MediumNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq21.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq31.00%LowDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 674 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq96.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq88.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq2.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq2.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 878 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq87.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq86.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq2.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq3.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhD 887 CDS C-to-U CCC=>CUC
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq88.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq85.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq3.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq3.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid ndhF 290 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
ndhG 50 CDS C-to-U UCC=>UUC
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq80.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq77.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq5.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq7.00%PoorDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana At3g20930 Plastid petL 5 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing77.78%HighSimilar32259001
Arabidopsis thaliana At3g20930 Plastid psbE 214 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana At3g20930 Plastid psbF 77 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana At3g20930 Plastid psbZ 50 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing94.44%HighSimilar32259001
Arabidopsis thaliana At3g20930 Plastid rpl23 89 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing14.29%PoorSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoA 200 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq81.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq40.00%MediumNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq22.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq22.00%LowDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing44.44%MediumDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoB 2432 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq96.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq81.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq8.00%PoorDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq20.00%LowDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoB 338 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq93.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq83.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq80.00%HighDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq77.00%HighDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing81.25%HighDecreased32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rpoB 551 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq96.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq83.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq25.00%LowDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq22.00%LowDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing33.33%LowDecreased32259001
Arabidopsis thaliana At3g20930 Plastid rpoC1 488 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing30.43%LowSimilar32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rps12 NA Intron C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq40.00%MediumNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq33.00%LowNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq0.00%UneditedAbsent23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq0.00%UneditedAbsent23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT3G20930 Chloroplast
Plastid
rps14 149 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNAGene specific RNA-seq92.00%HighNone23487777
Col-0WTControlControlControlNormalLeafNATotal plastid RNA-seq90.00%HighNone23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNAGene specific RNA-seq60.00%HighDecreased23487777
SALK_072648orrm1T-DNA InsertionT-DNA is inserted in the first exonHomozygous; KnockoutThe homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.LeafNATotal plastid RNA-seq75.00%HighDecreased23487777
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing50.00%MediumAbsent32259001
Arabidopsis thaliana At3g20930 Plastid rps14 80 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm1-1T-DNA insertion lines orrm1-1 (SALK_072648)NAHomozygousThe orrm1-1 mutant was actually slightly bigger than the Columbia wild type.Leaf4-weekRT-PCR and Sanger sequencing90.91%HighSimilar32259001
Zea mays GRMZM5G899787 Chloroplast atpA 1148 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlControlNormalLeaf14-21 daysDirect Sequencing of PCR ProductsNALowNone31077462
Zea mays GRMZM5G899787 Chloroplast matK 1258 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhA 1070 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhA 473 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAWTControlControlControlNormalLeaf14-21 daysDirect Sequencing of PCR ProductsNALowNone31077462
Zea mays GRMZM5G899787 Chloroplast ndhA 50 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 467 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay31.00%LowDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlControlNormalLeafNAPoisoned Primer Extension (PPE) Assay97.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 586 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay7.00%PoorDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlControlNormalLeafNAPoisoned Primer Extension (PPE) Assay98.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 737 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products10.00%PoorDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhB 830 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay65.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlControlNormalLeafNAPoisoned Primer Extension (PPE) Assay99.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhD 878 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay29.00%LowDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlControlNormalLeafNAPoisoned Primer Extension (PPE) Assay96.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast ndhG 347 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast petB 668 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast rpl2 2 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products70.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpl20 308 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
NAWTControlControlControlNormalLeaf14-21 daysDirect Sequencing of PCR ProductsNALowNone31077462
Zea mays GRMZM5G899787 Chloroplast rpoB 467 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoB 545 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay14.00%PoorDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
NAWTControlControlControlNormalLeafNAPoisoned Primer Extension (PPE) Assay89.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoB 560 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products60.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products90.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoB 617 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products40.00%MediumDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products80.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rpoC2 2774 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlControlNormalLeaf14-21 daysDirect Sequencing of PCR ProductsNAHighNone31077462
Zea mays GRMZM5G899787 Chloroplast rps14 80 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products90.00%HighDecreased23487777
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNAPoisoned Primer Extension (PPE) Assay90.00%HighDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
NAWTControlControlControlNormalLeafNAPoisoned Primer Extension (PPE) Assay97.00%HighNone23487777
Zea mays GRMZM5G899787 Chloroplast rps8 182 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products30.00%LowDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ycf3 185 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products0.00%UneditedAbsent23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Zea mays GRMZM5G899787 Chloroplast ycf3 44 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAZm-orrm1Mu InsertionComplementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).HeterozygousPale green phenotypeLeafNABulk sequencing of RT-PCR products20.00%LowDecreased23487777
NAWTControlControlControlNormalLeafNABulk sequencing of RT-PCR products100.00%CompleteNone23487777
Last update: Jul 2021 (version 1.0)