ORRM1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G20930

Plastid

accD

1568

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Plastid

accD

794

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast

accD

3'UTR

NC_000932

1568

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	81.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	67.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	1.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	4.00%	Poor	Decreased	23487777

Arabidopsis thaliana

AT3G20930

Plastid

atpF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

clpP

559

CDS

NC_000932
NA

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	90.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	24.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	40.00%	Medium	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	33.33%	Low	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Plastid

matK

640

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast

matK

706

CDS

NC_000932

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	89.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	7.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	1.00%	Poor	Decreased	23487777

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

1255

CDS

NC_000932
NA

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	100.00%	Complete	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	95.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	40.00%	Medium	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	42.00%	Medium	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	36.84%	Low	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Plastid

ndhB

1481

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Plastid

ndhB

149

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

467

CDS

NC_000932
NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	98.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	91.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	5.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	4.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

586

CDS

NC_000932
NA

C-to-U

CAU=>UAU
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	98.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	92.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	5.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	6.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

746

CDS

NC_000932
NA

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	98.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	94.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	46.00%	Medium	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	49.00%	Medium	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	50.00%	Medium	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

830

CDS

NC_000932
NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	94.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	84.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	28.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	22.00%	Low	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	18.75%	Poor	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

836

CDS

NC_000932
NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	88.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	73.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	4.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	8.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhB

872

CDS

NC_000932
NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	95.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	1.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	1.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

CDS

NC_000932
NA

C-to-U

ACG=>AUG
NA=>NA

T=>M
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	46.00%	Medium	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	54.00%	Medium	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	21.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	31.00%	Low	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Plastid

ndhD

383

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

674

CDS

NC_000932
NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	96.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	88.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	2.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	2.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

878

CDS

NC_000932
NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	87.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	86.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	2.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	3.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhD

887

CDS

NC_000932
NA

C-to-U

CCC=>CUC
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	88.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	85.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	3.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	3.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Plastid

ndhF

290

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

ndhG

CDS

NC_000932
NA

C-to-U

UCC=>UUC
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	80.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	77.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	5.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	7.00%	Poor	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Plastid

petL

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	77.78%	High	Similar	32259001

Arabidopsis thaliana

AT3G20930

Plastid

psbE

214

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Plastid

psbF

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	100.00%	Complete	Similar	32259001

Arabidopsis thaliana

AT3G20930

Plastid

psbZ

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	94.44%	High	Similar	32259001

Arabidopsis thaliana

AT3G20930

Plastid

rpl23

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	14.29%	Poor	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoA

200

CDS

NC_000932
NA

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	81.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	40.00%	Medium	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	22.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	22.00%	Low	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	44.44%	Medium	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoB

2432

CDS

NC_000932
NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	96.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	81.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	8.00%	Poor	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	20.00%	Low	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoB

338

CDS

NC_000932
NA

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	93.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	83.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	80.00%	High	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	77.00%	High	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	81.25%	High	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rpoB

551

CDS

NC_000932
NA

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	96.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	83.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	25.00%	Low	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	22.00%	Low	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	33.33%	Low	Decreased	32259001

Arabidopsis thaliana

AT3G20930

Plastid

rpoC1

488

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	30.43%	Low	Similar	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast

rps12

Intron

NC_000932

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	40.00%	Medium	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	33.00%	Low	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	0.00%	Unedited	Absent	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	0.00%	Unedited	Absent	23487777

Arabidopsis thaliana

AT3G20930

Plastid

rps12

Intron

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	0.00%	Unedited	Absent	32259001

Arabidopsis thaliana

AT3G20930

Chloroplast
Plastid

rps14

149

CDS

NC_000932
NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Gene specific RNA-seq	92.00%	High	None	23487777
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Total plastid RNA-seq	90.00%	High	None	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Gene specific RNA-seq	60.00%	High	Decreased	23487777
SALK_072648	orrm1	T-DNA Insertion	T-DNA is inserted in the first exon	Homozygous; Knockout	The homozygous mutant plant does not show any phenotypic defect compared with a Columbia wild-type plant when grown under growth-room conditions.	Leaf	NA	Total plastid RNA-seq	75.00%	High	Decreased	23487777
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	50.00%	Medium	Absent	32259001

Arabidopsis thaliana

AT3G20930

Plastid

rps14

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	orrm1-1	T-DNA insertion lines orrm1-1 (SALK_072648)	NA	Homozygous	The orrm1-1 mutant was actually slightly bigger than the Columbia wild type.	Leaf	4-week	RT-PCR and Sanger sequencing	90.91%	High	Similar	32259001

Zea mays

GRMZM5G899787

Chloroplast

atpA

1148

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

matK

1258

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhA

1070

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhA

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhB

467

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	31.00%	Low	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	97.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhB

586

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	7.00%	Poor	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	98.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhB

737

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	10.00%	Poor	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhB

830

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	60.00%	High	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	65.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	99.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhD

878

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	20.00%	Low	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	29.00%	Low	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	96.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ndhG

347

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

petB

668

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rpl2

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	70.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rpl20

308

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	20.00%	Low	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rpoB

467

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	60.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rpoB

545

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	14.00%	Poor	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	89.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rpoB

560

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	60.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rpoB

617

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	40.00%	Medium	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	80.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rpoC2

2774

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rps14

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	90.00%	High	Decreased	23487777
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Poisoned Primer Extension (PPE) Assay	90.00%	High	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	97.00%	High	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

rps8

182

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	30.00%	Low	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ycf3

185

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	0.00%	Unedited	Absent	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Zea mays

GRMZM5G899787

Chloroplast

ycf3

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Zm-orrm1	Mu Insertion	Complementation crosses between plants heterozygous for the two alleles (Zm-orrm1-1 and Zm-orrm1-2) yielded heteroallelic mutant progeny (Zm-orrm1-1/Zm-orrm1-2).	Heterozygous	Pale green phenotype	Leaf	NA	Bulk sequencing of RT-PCR products	20.00%	Low	Decreased	23487777
NA	WT	Control	Control	No mutant	Normal	Leaf	NA	Bulk sequencing of RT-PCR products	100.00%	Complete	None	23487777

Editing Factor:	ORRM1
Synonym:	ORGANELLE RRM PROTEIN 1
Description:	Transfection of Arabidopsis orrm1 mutant protoplasts with constructs encoding a region encompassing the RIP-RIP domain or a region spanning the RRM domain of ORRM1 demonstrated that the RRM domain is sufficient for the editing function of ORRM1 in vitro; According to a yeast two-hybrid assay, ORRM1 interacts selectively with pentatricopeptide transfactors via its RIP-RIP domain; Phylogenetic analysis reveals that the RRM in ORRM1 clusters with a clade of RRM proteins that are targeted to organelles; In the orrm1 mutant, 21 plastid RNA editing sites were affected but none are essential to photosynthesis; Morphological and physiological analyses showed that the orrm1 orrm6 double mutants had a smaller plant size, reduced chlorophyll contents, and decreased photosynthetic efficiency, similar to the orrm6 single mutants; Although the orrm1 orrm6 double mutants adopted the phenotype of the orrm6 single mutants, the total number of plastid RNA editing sites affected in the orrm1 orrm6 double mutants was the sum of the sites affected in the orrm1 and orrm6 single mutants
Protein Family:	Organelle RNA recognition motif-containing (ORRM)
Subclass:	NA
Physical Interaction:	CRR28; OTP82; OZ1, RARE1; CRR28; OTP82
Construct Structure:	PTP-RIP-ORRM, NA
Gene ID & Species:	AT3G20930 (Arabidopsis thaliana) GRMZM5G899787 (Zea mays)
Edited Gene(s):	accD clpP matK ndhB ndhD ndhG rpoA rpoB rps12 rps14 atpA ndhA petB rpl2 rpl20 rpoC2 rps8 ycf3 atpF ndhF petL psbE psbF psbZ rpl23 rpoC1
Editing Type(s):	C-to-U (174)
Publication(s):	[1] Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in the Arabidopsis thaliana plastid, Plant Direct, 2020. [doi:10.1002/pld3.213] [2] An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize, PNAS, 2013. [doi:10.1073/pnas.1220162110]

Summary

Editing Details