Plant Editosome Database
a curated database of RNA editosome in plants

EMP21 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: EMP21
Synonym: NA
Description: NA
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: GRMZM5G849971 (Zea mays)
Edited Gene(s): atp1    atp4    atp6    atp8    atp9    ccmB    ccmC    ccmFC    ccmFN    cob    cox1    cox2    cox3    matR    mttb    nad1    nad2    nad3    nad4    nad4L    nad5    nad6    nad7    nad9    rpl16    rps1    rps12    rps12_ct    rps13    rps2A    rps2b    rps3    rps4    rps7
Editing Type(s): C-to-U (1972)
Publication(s): [1] Empty Pericarp21 encodes a novel PPR-DYW protein that is required for mitochondrial RNA editing at multiple sites, complexes I and V biogenesis, and seed development in maize, PLoS Genet, 2019. [PMID=31374076]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays GRMZM5G849971 Mitochondrion atp1 30 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.37%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.60%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.59%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons28.32%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp1 1178 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.53%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.20%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.79%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.41%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp1 1292 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.46%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
Zea mays GRMZM5G849971 Mitochondrion atp1 1490 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.47%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.10%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.88%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.63%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp1 1499 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.46%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.49%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.45%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 40 CDS C-to-U CUA=>UUA L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.15%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.43%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.88%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.27%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 56 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.17%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.66%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.71%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 59 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.14%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.57%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.52%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.55%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 71 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.37%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.55%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.46%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.64%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 76 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.00%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.80%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.35%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.84%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 89 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.49%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.72%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.66%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.19%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 118 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.45%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.20%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.24%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.07%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 360 CDS C-to-U UGC=>UGU C=>C Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.59%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.24%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons64.41%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.48%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 407 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.63%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.94%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.10%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp4 428 CDS C-to-U ACU=>AUU T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.39%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.80%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.38%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.31%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 515 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.01%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.03%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.01%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.25%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 531 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.76%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.43%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.82%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.82%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 584 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.78%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.70%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.79%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.94%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 635 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.46%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.48%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.40%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.95%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 704 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.28%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.19%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.51%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.12%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 722 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.43%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.12%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.16%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.08%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 730 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.32%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.00%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.08%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.80%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 737 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.89%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.82%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.99%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.64%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 738 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.21%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.58%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.09%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.56%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 931 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.11%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.98%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.05%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.61%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 953 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.95%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.76%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.40%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.87%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 995 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.91%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.44%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.53%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.53%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 1050 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.49%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.01%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.87%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.31%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 1124 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.93%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.78%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.62%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.59%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 1132 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.28%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.11%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.45%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 1139 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.06%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.32%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.00%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.68%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 1148 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.37%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.33%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.36%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.19%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 1175 CDS C-to-U ACA=>AUA T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.55%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.28%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.83%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.18%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp6 1186 CDS C-to-U CAA=>UAA Q=>Stop codon Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.56%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.64%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.35%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp8 58 CDS C-to-U CUC=>UUC L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.13%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.41%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.65%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.70%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp8 123 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.47%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.77%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.82%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.28%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion atp8 200 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.45%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.27%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.09%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.44%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp8 436 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.37%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.01%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.46%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.79%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion atp8 437 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.69%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.30%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 20 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.13%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.03%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.21%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.88%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 82 CDS C-to-U CUC=>UUC L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.20%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.53%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.20%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.90%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 92 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.97%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.64%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.05%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.67%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 134 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.84%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.31%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.34%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.61%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 182 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.71%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.30%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.21%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.01%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 191 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.85%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.89%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.58%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.62%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 212 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.43%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.94%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.29%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion atp9 228 3'UTR C-to-U UUC=>UUU 3'UTR=>3'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.61%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.38%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.45%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.53%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 28 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.61%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.91%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.07%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.35%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 43 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.30%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.61%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.51%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.51%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 80 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.72%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.15%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.34%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 87 CDS C-to-U AUC=>AUU I=>I Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.19%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.61%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.44%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.80%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 93 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.42%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.49%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.00%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.68%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 128 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.90%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.82%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.43%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.52%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 137 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.92%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.54%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.61%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.21%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 149 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.04%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.94%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.47%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.93%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 154 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.76%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.13%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.09%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 172 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.77%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.20%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.98%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.12%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 179 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.08%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.83%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.14%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.98%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 181 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.15%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.73%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.12%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 183 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons42.87%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons33.17%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.94%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.32%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 193 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.87%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.07%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.00%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.87%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 194 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.82%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.05%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.54%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.83%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 286 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.70%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.48%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons52.72%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.26%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 304 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.55%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.61%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons55.15%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.37%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 337 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.17%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.87%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons41.92%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons36.50%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 338 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.10%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.44%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.48%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 367 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.85%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.20%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.18%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.90%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 379 CDS C-to-U CUA=>UUA L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.01%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.85%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.17%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 392 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.04%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.26%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.30%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.73%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 406 CDS C-to-U CUG=>UUG L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.33%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.15%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.45%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 424 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.67%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.09%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.12%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 428 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.27%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.88%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.66%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 467 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.83%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.42%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.34%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.96%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 475 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.55%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.93%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.54%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.23%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 476 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.27%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.59%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.54%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.02%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 485 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.42%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.40%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.22%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.42%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 503 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.48%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.15%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.95%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.47%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 512 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.48%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.94%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.26%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.73%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 514 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.22%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.97%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.05%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.25%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 551 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.02%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.12%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.63%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.91%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 554 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.42%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.01%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.31%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.93%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 566 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.65%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.65%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.75%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.40%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 569 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.40%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.77%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.78%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.15%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 572 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.84%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.38%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.54%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.00%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 583 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons31.74%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.59%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons20.51%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.83%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 596 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.93%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.42%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.73%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.11%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmB 611 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.12%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.00%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.09%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons41.77%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmC 103 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.29%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.73%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons3.31%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons3.71%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmC 227 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.10%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.20%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons2.73%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons1.90%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmC 446 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons7.68%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.18%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.92%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons1.69%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 38 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.93%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.25%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.21%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.34%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 39 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons34.35%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons20.40%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.68%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.96%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 50 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.45%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.18%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.60%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 52 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.13%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.33%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.34%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.28%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 87 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.84%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons27.42%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.56%MediumIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.76%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 103 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.57%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.18%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 119 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.14%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.48%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.10%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 122 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.57%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.96%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.57%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.53%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 123 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.13%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.89%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.46%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 146 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.06%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.96%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 160 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.37%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.14%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.43%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.49%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 306 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.53%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.34%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.87%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.75%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 325 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.56%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.93%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.17%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 397 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.61%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.73%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.11%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.05%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 543 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.26%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.86%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.34%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 799 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.73%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.79%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.14%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.28%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 866 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.33%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.32%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 906 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.17%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.75%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons41.11%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons41.73%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 966 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.07%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.84%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.05%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.73%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 1124 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.56%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.30%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.06%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 1144 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.26%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.56%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.29%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.60%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 1145 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.84%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.06%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.02%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.57%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 1219 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.56%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.86%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.18%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.19%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 1244 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.65%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.06%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.44%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.21%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFC 1300 CDS C-to-U CGA=>UGA R=>Stop codon Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.35%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.45%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.53%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.05%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 76 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.69%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.76%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.53%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.09%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 77 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.79%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons43.36%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.36%MediumIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.03%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 137 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.08%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.91%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.90%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.14%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 176 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.26%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.69%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.32%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.61%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 181 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.37%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.54%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.80%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.83%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 190 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.01%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.02%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.28%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.03%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 287 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.14%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.13%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.80%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.75%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 302 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.08%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.40%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.12%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.49%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 318 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.73%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons55.87%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.44%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.01%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 401 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.62%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.74%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.60%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.76%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 410 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.94%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.12%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.96%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.62%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 417 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.99%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.52%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.64%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.87%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 514 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons31.83%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.28%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.47%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons48.34%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 595 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.30%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.92%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.31%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 743 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.03%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.53%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.04%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.08%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 752 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.79%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.27%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.60%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 762 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.83%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.42%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons42.50%MediumIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons42.43%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 790 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.55%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.26%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.80%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.31%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 812 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.94%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.23%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.05%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.63%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 824 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.95%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.96%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.67%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.68%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 839 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.40%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.03%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.06%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.20%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 988 CDS C-to-U CGC=>UGC R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.92%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.89%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.26%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1214 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.93%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.50%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.51%LowIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.04%LowIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1325 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.70%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.74%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.97%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.59%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1342 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.23%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.15%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.76%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.78%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1357 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.59%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.02%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.07%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.50%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1375 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.36%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.92%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.46%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.40%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1408 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.24%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.49%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.78%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.66%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1419 CDS C-to-U GCC=>GCU A=>A Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.33%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.02%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.62%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.22%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1469 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.62%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.91%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.23%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.61%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1474 CDS C-to-U CUG=>UUG L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons7.94%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.89%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.49%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.14%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1489 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.14%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.09%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.48%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.96%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1492 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.35%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.20%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.14%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.21%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1493 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.61%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.49%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.23%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.47%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1505 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.02%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.72%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.62%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.30%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1540 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.94%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.81%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.64%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.55%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1553 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.50%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.52%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.52%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.34%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1588 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.99%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.17%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.60%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.95%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1709 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.34%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.03%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.25%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.73%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion ccmFN 1918 3'UTR C-to-U CAU=>UAU 3'UTR=>3'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.51%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.94%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.09%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.29%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 286 CDS C-to-U CUC=>UUC L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.81%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.83%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.50%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 298 CDS C-to-U CAC=>UAC H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.98%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.84%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.84%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.71%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 325 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.05%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.58%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 419 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.47%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.98%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.26%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.42%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 564 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.22%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.17%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.84%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cob 568 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.48%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.49%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.82%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 580 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.87%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.12%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.62%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.70%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 680 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.32%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.19%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.54%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.38%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 715 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.20%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.02%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.94%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.41%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 725 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.88%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.33%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.56%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.24%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 808 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.36%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.10%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.72%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.25%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 853 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.53%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.43%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.76%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.21%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 908 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.64%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.52%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.68%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.36%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 982 CDS C-to-U CAC=>UAC H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.76%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.19%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.25%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.22%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 1098 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.33%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons62.66%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.03%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.43%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion cob 1124 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.59%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.14%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.97%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cob 1171 3'UTR C-to-U UCA=>UUA 3'UTR=>3'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.25%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.00%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.80%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.31%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox1 15 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.04%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.15%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.57%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.04%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox1 1402 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.50%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.10%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.31%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.33%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox1 1405 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.01%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.91%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.67%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.24%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox1 1433 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.28%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.84%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.21%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox1 1489 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.01%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.24%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.20%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.07%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 14 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.95%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.32%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.12%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 30 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.24%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.60%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.02%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.06%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 31 CDS C-to-U CUC=>UUC L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.46%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons53.77%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons42.04%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.30%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 77 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.01%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.31%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.73%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 144 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.86%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.64%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.53%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.62%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 162 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.35%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.19%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.82%LowIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.55%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 167 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.53%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.45%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.79%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 169 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.70%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.42%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.41%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.66%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 259 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.52%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.55%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.58%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 284 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.51%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.65%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.54%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.15%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 385 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.52%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.09%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.20%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.21%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 449 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.92%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.54%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 466 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.97%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.87%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.58%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 467 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.32%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.17%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.22%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.60%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 482 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.92%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.58%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.92%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 550 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.70%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.72%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.02%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 563 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.86%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.12%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.45%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.92%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 587 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.42%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.36%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.88%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.14%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 620 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.20%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.80%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.46%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 638 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons36.61%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.04%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.44%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.56%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox2 704 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.40%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.45%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.48%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.59%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 69 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.31%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons48.60%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.40%PoorDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.60%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 245 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.92%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.17%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.56%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.42%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 257 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.00%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.45%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.43%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons27.00%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 289 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.53%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.82%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.91%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.05%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 311 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.90%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons64.27%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.80%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons35.43%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 314 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.04%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.39%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons38.36%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.40%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 413 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.50%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.52%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.24%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.93%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 422 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.39%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.77%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.98%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.82%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 527 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.36%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.55%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.72%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons52.26%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 566 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.04%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.48%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.67%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.77%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 754 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.44%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.99%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.20%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.36%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion cox3 764 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.00%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.71%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons48.07%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.91%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion matR 17 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.39%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.09%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons41.60%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.84%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 86 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.99%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.38%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.72%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.20%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 92 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.80%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.13%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.80%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.77%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 295 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.81%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.42%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons27.44%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.43%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 296 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.13%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.75%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.16%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1124 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.97%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.24%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.90%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.47%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1730 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.82%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.14%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.08%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1751 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.68%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.06%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.91%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.73%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1785 CDS C-to-U UAC=>UAU Y=>Y Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.97%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.50%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.71%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.80%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1877 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.27%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.96%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.11%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.58%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1885 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.81%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.38%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.24%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1895 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.88%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.60%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.99%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.05%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion matR 1905 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.88%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.31%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.88%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.83%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion mttb 127 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.35%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.10%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons23.75%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.39%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion mttb 205 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons7.66%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.11%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.92%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion mttb 215 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.83%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.31%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.06%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion mttb 373 CDS C-to-U CGC=>UGC R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.13%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.45%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.19%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons19.67%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion mttb 532 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.26%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.47%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons35.68%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons33.92%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 2 CDS C-to-U ACG=>ATG T=>Start codo Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.67%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.80%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.93%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.24%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 138 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.51%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.11%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.71%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.28%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 215 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.88%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.22%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.79%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.01%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 307 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.69%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.17%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.71%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.27%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 308 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.56%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.32%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.60%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.92%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 490 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.66%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 492 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.07%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.41%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 500 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.55%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.85%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 536 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.25%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.64%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 537 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.22%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.45%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.71%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.16%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 555 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.30%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.98%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.15%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.50%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 571 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.18%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.58%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.70%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.49%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 577 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.69%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.19%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.39%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 580 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.58%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.38%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.53%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 608 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.81%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.71%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 635 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.41%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.60%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 674 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 679 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons19.43%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.94%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.93%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons20.21%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 734 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 743 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.57%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 755 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.48%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 779 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.34%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.47%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.64%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.44%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 823 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.09%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.86%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.57%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.41%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 835 CDS C-to-U CUA=>UUA L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.46%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.85%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.82%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.40%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 898 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.51%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.70%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.97%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 928 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.02%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.72%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.60%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.79%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 937 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.59%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.32%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.24%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad1 953 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.18%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.30%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.18%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 -8 5'UTR C-to-U UCG=>UUG 5'UTR=>5'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.27%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.86%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.12%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 26 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.12%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.78%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.26%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 303 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons23.88%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons19.71%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons33.77%LowIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.01%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 308 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.69%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.64%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 356 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.07%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.75%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.61%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.51%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 361 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.69%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 367 CDS C-to-U CGC=>UGC R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.36%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.08%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.56%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 388 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 394 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.99%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 453 CDS C-to-U AUC=>AUU I=>I Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.96%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.55%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.01%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.21%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 497 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.96%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 523 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.56%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.75%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 621 CDS C-to-U AUC=>AUU I=>I Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 677 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.15%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.75%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 788 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.63%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.24%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.85%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 800 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 809 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.64%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 920 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.63%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 928 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 958 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.73%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.72%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.85%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 962 CDS C-to-U ACU=>AUU T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.14%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.02%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.65%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1028 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.92%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1058 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1246 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1247 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.22%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.85%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1276 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.38%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.56%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1298 CDS C-to-U GCG=>GUG A=>V Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.56%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.94%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1400 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.72%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.90%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.37%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1403 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.58%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.57%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.38%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.83%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1408 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.37%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.53%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.52%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.78%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1409 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.49%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.99%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.51%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1416 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.62%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.55%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.55%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.68%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad2 1457 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.85%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.71%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 5 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons33.11%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.21%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.15%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.54%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 39 CDS C-to-U AUC=>AUU I=>I Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.86%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.32%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.35%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons16.81%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 44 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.27%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.05%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.16%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.12%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 61 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.13%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.50%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.28%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons20.61%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 62 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.10%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons41.91%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.81%PoorDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.60%PoorDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 80 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons52.82%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.65%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.98%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.86%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 137 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.55%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.52%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.52%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.96%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 138 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.45%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.81%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.39%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 146 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons55.15%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.35%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.08%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons28.62%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 185 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.09%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.42%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.46%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.04%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 190 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.12%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.85%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons43.49%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 208 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons48.21%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.01%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.38%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.73%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 209 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.49%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.61%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.78%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.55%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 215 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.05%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.42%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons19.72%PoorDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons23.07%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 230 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.23%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons38.38%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.13%PoorDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons18.05%PoorDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 247 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons42.43%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.03%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.44%PoorDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.73%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 251 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.16%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.79%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.91%PoorDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.37%PoorDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 275 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.80%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons18.36%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 317 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.68%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.25%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.42%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.52%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 344 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons55.10%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.96%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons28.25%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons33.54%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad3 349 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.28%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.16%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons31.31%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.86%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 -7 5'UTR C-to-U UUC=>UUU 5'UTR=>5'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons23.26%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.27%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.01%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.87%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 44 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.08%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.38%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.74%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.88%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 74 CDS C-to-U ACU=>AUU T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.62%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.26%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.85%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 77 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.79%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.53%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.66%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 84 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.54%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons23.18%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.81%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.99%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 107 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.79%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 154 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.75%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.20%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.85%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.57%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 156 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.06%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.22%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.20%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.23%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 158 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.63%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.76%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 164 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.27%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.56%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.29%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.88%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 166 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.94%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.99%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 197 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.81%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.85%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 317 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.99%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.96%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 362 CDS C-to-U ACA=>AUA T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 376 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 401 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.77%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 416 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.69%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.68%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.64%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 433 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.33%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.28%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.79%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.84%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 437 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.45%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 449 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.66%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.42%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 819 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons62.77%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.15%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.13%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.57%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 977 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.33%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.13%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.62%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.55%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 1373 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.17%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.54%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.72%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 1405 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.69%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.63%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 1417 CDS C-to-U CAC=>UAC H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.53%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.14%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.01%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 1432 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.91%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.24%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.50%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.01%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 1433 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.36%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.49%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.71%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4 1452 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.97%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.23%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.77%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.59%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4L 41 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.17%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.26%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.71%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4L 110 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.82%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.74%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.81%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad4L 179 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.26%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.16%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons18.02%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.88%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1183 CDS C-to-U CUA=>UUA L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.11%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.11%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.22%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.63%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1490 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.84%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.92%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1494 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.30%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons28.74%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons52.70%MediumIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.23%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1550 CDS C-to-U ACA=>AUA T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.79%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1567 CDS C-to-U CUG=>UUG L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.63%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.58%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons64.41%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.93%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1580 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.57%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.75%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.75%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1589 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.90%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.79%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1665 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.16%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.97%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.99%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.44%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1859 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.84%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.52%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1895 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1900 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.50%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.75%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1901 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.51%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.35%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.73%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1916 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.25%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.00%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.79%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.65%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 1918 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.64%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.02%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.66%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad5 2005 CDS C-to-U CAA=>UAA Q=>Stop codon Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.10%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons13.83%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.30%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.55%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 -45 5'UTR C-to-U CCC=>UCC 5'UTR=>5'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.98%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.03%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.36%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons12.06%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 -3 CDS C-to-U CCA=>UCA P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons25.51%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.53%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons27.15%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.99%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 25 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.51%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.86%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.97%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.68%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 26 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.43%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.28%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.46%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.98%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 88 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.37%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.13%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.43%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.33%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 89 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.10%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.95%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.28%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.11%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 138 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons31.93%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons37.26%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons18.44%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.29%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 146 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.94%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.56%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons62.34%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 159 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons38.46%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.91%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.60%PoorDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.45%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 161 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.72%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.60%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.93%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.99%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 169 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.05%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.56%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.58%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 191 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.60%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.26%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.31%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.97%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 196 CDS C-to-U CUA=>UUA L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons16.54%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.02%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.94%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.12%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 446 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.31%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.32%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.27%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.92%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad6 569 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.21%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.92%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.77%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.60%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 -7 5'UTR C-to-U UUC=>UUU 5'UTR=>5'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.93%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.55%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons29.42%LowIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons34.07%LowIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 38 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.41%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.71%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 44 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.91%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 45 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.62%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 77 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.68%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.47%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 83 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.73%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.96%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.68%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 99 CDS C-to-U AAC=>AAU N=>N Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.54%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.35%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.70%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.14%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 137 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.63%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.74%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.65%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.73%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 209 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.91%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.84%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.58%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 224 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.79%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.06%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 244 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.84%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.52%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 251 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.92%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 255 CDS C-to-U GCC=>GCU A=>A Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons34.46%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.85%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons48.41%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.82%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 315 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.96%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 335 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 344 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.99%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.97%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.51%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 353 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.99%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.96%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.69%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 383 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.83%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.95%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.91%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 445 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.45%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.78%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.75%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 533 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.27%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.08%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.02%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.89%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 578 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.67%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.89%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.90%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 679 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.21%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.69%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.06%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 698 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.97%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.29%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.63%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 724 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.98%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 734 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.26%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.45%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.45%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.59%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 739 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.81%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.91%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.68%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.41%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 769 CDS C-to-U CGC=>UGC R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons100.00%CompleteSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 836 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.81%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.82%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.87%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 926 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.88%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.94%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.65%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.75%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 973 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.62%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.71%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.57%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.40%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 1088 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.84%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.77%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.84%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.67%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 1103 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.42%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.80%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.96%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad7 1124 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.93%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.96%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.38%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 14 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.81%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.66%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.75%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.54%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 92 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.25%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.79%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.35%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.77%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 111 CDS C-to-U UAC=>UAU Y=>Y Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.18%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons14.39%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.87%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons18.98%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 113 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.45%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.00%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.20%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.43%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 167 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.18%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.44%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.17%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.40%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 190 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.14%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.97%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.40%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.03%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 223 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.49%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.55%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.52%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.25%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 298 CDS C-to-U CCG=>UCG P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.13%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.88%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.07%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.19%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 311 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.31%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.48%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.62%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.24%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 328 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.78%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.46%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.50%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 356 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.72%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.75%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.80%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.13%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 368 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.28%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.90%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.27%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.43%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion nad9 398 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.47%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.11%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.46%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.56%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 48 CDS C-to-U CUC=>CUU L=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.47%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.61%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.33%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.71%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 79 CDS C-to-U CAG=>UAG Q=>Stop codon Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.08%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.65%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.44%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.14%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 93 CDS C-to-U UGC=>UGU C=>C Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.83%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.57%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.58%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.20%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 184 CDS C-to-U CGC=>UGC R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.30%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.79%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.70%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.86%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 225 CDS C-to-U GGC=>GGU G=>G Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.60%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.48%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.48%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.89%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 227 CDS C-to-U ACC=>AUC T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.95%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.49%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.01%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.39%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 228 CDS C-to-U ACC=>ACU T=>T Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons38.78%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.38%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons28.29%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.17%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 287 CDS C-to-U ACA=>AUA T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.96%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.65%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.11%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.09%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 355 CDS C-to-U CUC=>UUC L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.80%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.81%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.43%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.65%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 444 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons62.41%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.61%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.82%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.90%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 458 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.93%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.61%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.02%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.28%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 524 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.67%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.05%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.21%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.50%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rpl16 530 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.80%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.60%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.54%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.99%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps1 107 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.20%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.23%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons28.98%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons36.97%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps1 377 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.21%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.34%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.65%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.55%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps1 505 CDS C-to-U CAA=>UAA Q=>Stop codon Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.65%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.07%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons21.78%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.47%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps12 71 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.91%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.87%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons33.98%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.70%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps12 196 CDS C-to-U CAC=>UAC H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.46%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.94%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.69%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.38%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps12 221 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.38%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.05%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.93%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons55.54%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps12 269 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.06%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.77%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.26%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.07%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps12 284 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.40%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.78%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.45%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.08%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps12 289 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.09%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons36.24%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.99%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.02%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps12_ct 418 Intronic C-to-U CAA=>UAA Intronic=>Intronic Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.15%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.45%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons39.39%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.03%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps13 5 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.84%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.71%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.26%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.85%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps13 26 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.29%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.31%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.06%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps13 56 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons45.25%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.64%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons36.62%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.36%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps13 100 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.73%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.58%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.89%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.82%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps13 256 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.96%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.22%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons43.46%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons48.74%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps13 287 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.23%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons65.96%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.52%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons53.62%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps2A 200 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.78%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.03%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.67%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.45%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps2A 449 CDS C-to-U GCA=>GUA A=>V Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.56%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons55.65%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.08%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.47%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps2A 514 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.13%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.30%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons90.77%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.41%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps2A 541 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.19%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.66%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.42%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.71%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps2A 548 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.11%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.70%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.59%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.82%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps2b 15 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.14%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.19%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons44.12%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.72%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps2b 206 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.72%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons85.89%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons73.95%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.72%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps2b 455 CDS C-to-U GCA=>GUA A=>V Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.49%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.95%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons94.04%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.17%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps2b 510 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.76%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.50%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons19.84%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons31.89%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps2b 526 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons8.67%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons10.83%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons9.04%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons15.95%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps2b 550 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.24%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.77%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.76%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.88%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps2b 557 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.30%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons93.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.92%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.14%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 64 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.58%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.01%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.86%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.66%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 69 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.26%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.74%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons57.00%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.13%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 92 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.86%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.42%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.45%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.49%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 314 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons22.55%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons20.38%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons43.21%MediumIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons43.94%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 497 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons32.41%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons31.05%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons51.51%MediumIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.41%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 503 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.52%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.73%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.97%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.48%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 707 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons34.34%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons34.51%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.01%HighIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.44%HighIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 878 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.10%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.87%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons16.26%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons17.84%PoorSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 1019 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.00%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons69.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.78%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.08%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 1066 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons19.78%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons19.77%PoorNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons41.83%MediumIncreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.95%MediumIncreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 1576 CDS C-to-U CCU=>UCU P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.12%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.96%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons89.45%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.46%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 1607 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.09%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.37%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.85%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.81%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps3 1621 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons98.95%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons99.16%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.85%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons97.73%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 -9 5'UTR C-to-U CAA=>UAA 5'UTR=>5'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.19%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.62%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons40.31%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons55.58%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 38 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.38%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.76%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons67.20%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons64.53%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 49 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.05%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.26%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons88.01%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons96.11%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 164 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.61%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons64.67%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.17%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.18%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 278 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.59%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.55%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.03%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons95.83%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 290 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons71.54%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.43%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons64.62%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.97%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 335 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.58%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.64%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.85%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons91.06%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 482 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons66.09%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons58.90%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons68.58%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.11%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 549 CDS C-to-U UUC=>UUU F=>F Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons43.85%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons49.91%MediumNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons23.55%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons35.18%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 870 CDS C-to-U ACC=>ACU T=>T Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons16.76%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons24.63%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons0.00%UneditedAbsent31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 915 CDS C-to-U AUC=>AUU I=>I Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons83.17%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.60%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.12%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons86.69%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 944 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.98%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.41%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons56.70%MediumSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons59.21%MediumSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 955 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons80.44%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons74.93%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons78.85%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.62%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 980 CDS C-to-U UCU=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.06%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons75.34%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons47.85%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons50.47%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 1031 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons87.11%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.12%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.62%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons92.30%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 1040 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons84.99%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.42%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons81.44%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.62%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps4 1045 CDS C-to-U CGG=>UGG R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.92%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons76.65%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons72.88%HighSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons79.24%HighSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps7 -114 5'UTR C-to-U CCU=>UCU 5'UTR=>5'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.84%LowNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.98%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons26.24%LowSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons23.34%LowSimilar31374076
Zea mays GRMZM5G849971 Mitochondrion rps7 -68 5'UTR C-to-U UCG=>UUG 5'UTR=>5'UTR Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons64.09%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons70.86%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons52.32%MediumDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons46.97%MediumDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps7 277 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons54.65%MediumNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons61.13%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons31.76%LowDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons30.39%LowDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps7 332 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons77.31%HighNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons82.97%HighNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons60.39%HighDecreased31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons63.76%HighDecreased31374076
Zea mays GRMZM5G849971 Mitochondrion rps7 520 CDS C-to-U CUU=>UUU L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22WT-1No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.99%PoorNone31374076
W22WT-2No treatment No treatmentNo treatmentNormalKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons36.95%LowNone31374076
W22emp21-1Mutator (Mu) insertionA Mu insertion at +280 bp from the translation start codon in GRMZM5G849971HeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons11.72%PoorSimilar31374076
W22emp21-2Mutator (Mu) insertionThe Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codonHeterozygousEmpty pericarpKernel12 DAPSTS-PCRseq, Direct sequencing of RT-PCR amplicons20.87%LowSimilar31374076
Last update: Jul 2021 (version 1.0)