EMP21 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM5G849971

Mitochondrion

atp1

1178

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.53%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.20%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.79%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.41%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp1

1292

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.46%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp1

1490

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.47%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.10%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.88%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.63%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp1

1499

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.46%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.49%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.45%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp1

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.37%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.60%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.59%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	28.32%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

118

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.45%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.20%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.24%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.07%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

360

CDS

C-to-U

UGC=>UGU

C=>C

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.59%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.24%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	64.41%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.48%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.15%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.43%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.88%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.27%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

407

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.63%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.94%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.10%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

428

CDS

C-to-U

ACU=>AUU

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.39%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.80%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.38%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.31%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.17%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.66%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.71%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.14%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.57%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.52%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.55%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.37%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.55%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.46%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.64%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.00%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.80%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.35%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.84%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp4

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.49%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.72%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.66%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.19%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

1050

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.49%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.01%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.87%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.31%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

1124

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.93%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.78%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.62%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.59%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

1132

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.28%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.11%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.45%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

1139

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.06%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.32%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.00%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.68%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

1148

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.37%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.33%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.36%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.19%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

1175

CDS

C-to-U

ACA=>AUA

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.55%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.28%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.83%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.18%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

1186

CDS

C-to-U

CAA=>UAA

Q=>Stop codon

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.56%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.64%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.35%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

515

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.01%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.03%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.01%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.25%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

531

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.76%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.43%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.82%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.82%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

584

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.78%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.70%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.79%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.94%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

635

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.46%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.48%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.40%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.95%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

704

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.28%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.19%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.51%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.12%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

722

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.43%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.12%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.16%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.08%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

730

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.32%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.00%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.08%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.80%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

737

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.89%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.82%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.99%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.64%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

738

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.21%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.58%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.09%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.56%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

931

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.11%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.98%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.05%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.61%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

953

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.95%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.76%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.40%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.87%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp6

995

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.91%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.44%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.53%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.53%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp8

123

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.47%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.77%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.82%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.28%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp8

200

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.45%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.27%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.09%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.44%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp8

436

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.37%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.01%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.46%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.79%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp8

437

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.69%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.30%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp8

CDS

C-to-U

CUC=>UUC

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.13%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.41%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.65%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.70%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

134

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.84%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.31%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.34%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.61%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

182

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.71%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.30%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.21%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.01%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

191

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.85%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.89%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.58%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.62%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.13%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.03%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.21%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.88%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

212

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.43%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.94%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.29%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

CDS

C-to-U

CUC=>UUC

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.20%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.53%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.20%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.90%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.97%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.64%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.05%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.67%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

atp9

3'UTR

228

C-to-U

UUC=>UUU

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.61%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.38%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.45%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.53%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

128

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.90%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.82%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.43%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.52%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

137

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.92%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.54%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.61%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.21%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

149

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.04%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.94%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.47%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.93%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

154

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.76%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.13%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.09%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

172

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.77%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.20%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.98%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.12%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

179

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.08%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.83%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.14%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.98%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

181

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.15%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.73%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.12%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

183

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	42.87%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	33.17%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.94%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.32%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

193

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.87%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.07%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.00%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.87%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

194

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.82%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.05%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.54%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.83%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.61%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.91%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.07%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.35%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

286

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.70%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.48%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	52.72%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.26%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

304

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.55%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.61%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	55.15%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.37%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

337

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.17%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.87%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	41.92%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	36.50%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

338

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.10%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.44%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.48%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

367

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.85%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.20%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.18%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.90%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

379

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.01%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.85%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.17%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

392

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.04%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.26%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.30%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.73%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

406

CDS

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.33%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.15%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.45%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

424

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.67%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.09%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.12%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

428

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.27%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.88%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.66%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.30%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.61%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.51%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.51%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

467

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.83%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.42%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.34%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.96%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

475

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.55%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.93%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.54%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.23%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

476

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.27%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.59%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.54%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.02%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

485

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.42%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.40%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.22%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.42%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

503

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.48%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.15%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.95%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.47%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

512

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.48%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.94%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.26%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.73%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

514

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.22%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.97%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.05%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.25%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

551

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.02%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.12%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.63%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.91%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

554

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.42%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.01%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.31%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.93%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

566

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.65%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.65%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.75%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.40%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

569

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.40%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.77%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.78%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.15%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

572

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.84%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.38%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.54%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.00%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

583

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	31.74%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.59%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	20.51%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.83%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

596

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.93%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.42%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.73%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.11%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

611

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.12%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.00%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.09%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	41.77%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.72%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.15%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.34%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.19%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.61%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.44%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.80%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmB

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.42%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.49%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.00%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.68%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmC

103

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.29%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.73%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	3.31%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	3.71%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmC

227

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.10%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.20%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	2.73%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	1.90%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmC

446

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	7.68%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.18%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.92%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	1.69%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

103

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.57%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.18%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

1124

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.56%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.30%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.06%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

1144

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.26%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.56%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.29%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.60%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

1145

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.84%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.06%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.02%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.57%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

119

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.14%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.48%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.10%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

1219

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.56%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.86%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.18%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.19%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

122

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.57%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.96%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.57%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.53%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

123

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.13%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.89%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.46%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

1244

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.65%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.06%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.44%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.21%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

1300

CDS

C-to-U

CGA=>UGA

R=>Stop codon

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.35%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.45%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.53%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.05%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

146

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.06%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.96%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

160

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.37%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.14%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.43%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.49%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

306

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.53%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.34%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.87%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.75%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

325

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.56%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.93%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.17%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.93%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.25%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.21%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.34%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	34.35%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	20.40%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.68%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.96%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

397

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.61%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.73%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.11%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.05%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.45%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.18%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.60%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.13%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.33%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.34%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.28%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

543

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.26%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.86%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.34%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

799

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.73%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.79%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.14%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.28%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

866

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.33%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.32%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.84%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	27.42%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.56%	Medium	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.76%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

906

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.17%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.75%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	41.11%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	41.73%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFc

966

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.07%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.84%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.05%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.73%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1214

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.93%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.50%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.51%	Low	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.04%	Low	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1325

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.70%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.74%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.97%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.59%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1342

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.23%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.15%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.76%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.78%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1357

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.59%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.02%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.07%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.50%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

137

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.08%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.91%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.90%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.14%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1375

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.36%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.92%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.46%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.40%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1408

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.24%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.49%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.78%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.66%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1419

CDS

C-to-U

GCC=>GCU

A=>A

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.33%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.02%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.62%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.22%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1469

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.62%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.91%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.23%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.61%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1474

CDS

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	7.94%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.89%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.49%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.14%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1489

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.14%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.09%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.48%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.96%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1492

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.35%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.20%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.14%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.21%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1493

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.61%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.49%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.23%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.47%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1505

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.02%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.72%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.62%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.30%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1540

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.94%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.81%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.64%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.55%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1553

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.50%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.52%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.52%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.34%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1588

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.99%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.17%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.60%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.95%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

1709

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.34%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.03%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.25%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.73%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

176

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.26%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.69%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.32%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.61%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

181

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.37%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.54%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.80%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.83%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

190

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.01%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.02%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.28%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.03%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

287

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.14%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.13%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.80%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.75%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

302

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.08%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.40%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.12%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.49%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

318

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.73%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	55.87%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.44%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.01%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

401

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.62%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.74%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.60%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.76%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

410

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.94%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.12%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.96%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.62%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

417

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.99%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.52%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.64%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.87%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

514

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	31.83%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.28%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.47%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	48.34%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

595

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.30%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.92%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.31%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

743

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.03%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.53%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.04%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.08%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

752

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.79%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.27%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.60%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.69%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.76%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.53%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.09%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

762

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.83%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.42%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	42.50%	Medium	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	42.43%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.79%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	43.36%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.36%	Medium	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.03%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

790

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.55%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.26%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.80%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.31%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

812

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.94%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.23%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.05%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.63%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

824

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.95%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.96%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.67%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.68%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

839

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.40%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.03%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.06%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.20%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

988

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.92%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.89%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.26%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

ccmFn

3'UTR

1918

C-to-U

CAU=>UAU

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.51%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.94%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.09%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.29%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

1098

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.33%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	62.66%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.03%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.43%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

1124

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.59%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.14%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.97%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

286

CDS

C-to-U

CUC=>UUC

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.81%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.83%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.50%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

298

CDS

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.98%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.84%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.84%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.71%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

325

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.05%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.58%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

419

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.47%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.98%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.26%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.42%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

564

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.22%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.17%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.84%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

568

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.48%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.49%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.82%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

580

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.87%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.12%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.62%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.70%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

680

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.32%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.19%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.54%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.38%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

715

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.20%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.02%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.94%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.41%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

725

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.88%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.33%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.56%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.24%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

808

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.36%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.10%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.72%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.25%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

853

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.53%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.43%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.76%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.21%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

908

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.64%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.52%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.68%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.36%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

982

CDS

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.76%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.19%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.25%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.22%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cob

3'UTR

1171

C-to-U

UCA=>UUA

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.25%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.00%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.80%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.31%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox1

1402

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.50%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.10%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.31%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.33%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox1

1405

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.01%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.91%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.67%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.24%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox1

1433

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.28%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.84%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.21%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox1

1489

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.01%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.24%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.20%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.07%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox1

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.04%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.15%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.57%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.04%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.95%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.32%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.12%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

144

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.86%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.64%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.53%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.62%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

162

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.35%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.19%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.82%	Low	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.55%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

167

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.53%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.45%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.79%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

169

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.70%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.42%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.41%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.66%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

259

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.52%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.55%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.58%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

284

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.51%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.65%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.54%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.15%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.24%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.60%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.02%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.06%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

CDS

C-to-U

CUC=>UUC

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.46%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	53.77%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	42.04%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.30%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

385

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.52%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.09%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.20%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.21%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

449

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.92%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.54%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

466

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.97%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.87%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.58%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

467

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.32%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.17%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.22%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.60%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

482

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.92%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.58%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.92%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

550

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.70%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.72%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.02%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

563

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.86%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.12%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.45%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.92%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

587

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.42%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.36%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.88%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.14%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

620

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.20%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.80%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.46%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

638

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	36.61%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.04%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.44%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.56%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

704

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.40%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.45%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.48%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.59%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox2

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.01%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.31%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.73%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

245

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.92%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.17%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.56%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.42%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

257

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.00%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.45%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.43%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	27.00%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

289

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.53%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.82%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.91%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.05%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

311

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.90%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	64.27%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.80%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	35.43%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

314

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.04%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.39%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	38.36%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.40%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

413

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.50%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.52%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.24%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.93%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

422

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.39%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.77%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.98%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.82%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

527

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.36%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.55%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.72%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	52.26%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

566

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.04%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.48%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.67%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.77%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.31%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	48.60%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.40%	Poor	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.60%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

754

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.44%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.99%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.20%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.36%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

cox3

764

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.00%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.71%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	48.07%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.91%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1124

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.97%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.24%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.90%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.47%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.39%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.09%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	41.60%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.84%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1730

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.82%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.14%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.08%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1751

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.68%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.06%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.91%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.73%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1785

CDS

C-to-U

UAC=>UAU

Y=>Y

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.97%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.50%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.71%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.80%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1877

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.27%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.96%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.11%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.58%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1885

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.81%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.38%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.24%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1895

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.88%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.60%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.99%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.05%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

1905

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.88%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.31%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.88%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.83%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

295

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.81%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.42%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	27.44%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.43%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

296

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.13%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.75%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.16%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.99%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.38%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.72%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.20%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

matR

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.80%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.13%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.80%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.77%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

mttB

127

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.35%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.10%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	23.75%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.39%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

mttB

205

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	7.66%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.11%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.92%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

mttB

215

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.83%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.31%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.06%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

mttB

373

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.13%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.45%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.19%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	19.67%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

mttB

532

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.26%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.47%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	35.68%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	33.92%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

138

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.51%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.11%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.71%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.28%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

CDS

C-to-U

ACG=>AUG

T=>Start codo

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.67%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.80%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.93%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.24%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

215

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.88%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.22%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.79%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.01%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

307

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.69%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.17%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.71%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.27%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

308

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.56%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.32%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.60%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.92%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

490

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.66%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

492

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.07%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.41%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

500

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.55%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.85%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

536

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.25%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.64%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

537

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.22%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.45%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.71%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.16%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

555

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.30%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.98%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.15%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.50%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

571

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.18%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.58%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.70%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.49%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

577

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.69%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.19%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.39%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

580

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.58%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.38%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.53%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

608

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.81%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.71%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

635

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.41%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.60%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

674

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

679

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	19.43%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.94%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.93%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	20.21%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

734

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

743

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.57%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

755

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.48%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

779

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.34%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.47%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.64%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.44%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

823

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.09%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.86%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.57%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.41%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

835

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.46%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.85%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.82%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.40%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

898

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.51%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.70%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.97%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

928

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.02%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.72%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.60%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.79%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

937

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.59%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.32%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.24%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad1

953

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.18%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.30%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.18%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1028

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.92%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1058

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1246

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1247

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.22%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.85%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1276

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.38%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.56%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1298

CDS

C-to-U

GCG=>GUG

A=>V

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.56%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.94%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1400

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.72%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.90%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.37%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1403

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.58%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.57%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.38%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.83%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1408

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.37%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.53%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.52%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.78%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1409

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.49%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.99%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.51%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1416

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.62%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.55%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.55%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.68%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

1457

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.85%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.71%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.12%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.78%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.26%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

303

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	23.88%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	19.71%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	33.77%	Low	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.01%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

308

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.69%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.64%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

356

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.07%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.75%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.61%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.51%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

361

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.69%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

367

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.36%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.08%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.56%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

388

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

394

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.99%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

453

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.96%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.55%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.01%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.21%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

497

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.96%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

523

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.56%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.75%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

621

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

677

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.15%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.75%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

788

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.63%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.24%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.85%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

800

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

809

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.64%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

920

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.63%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

928

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

958

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.73%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.72%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.85%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

962

CDS

C-to-U

ACU=>AUU

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.14%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.02%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.65%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad2

5'UTR

-8

C-to-U

UCG=>UUG

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.27%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.86%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.12%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

137

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.55%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.52%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.52%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.96%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

138

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.45%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.81%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.39%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

146

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	55.15%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.35%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.08%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	28.62%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

185

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.09%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.42%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.46%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.04%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

190

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.12%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.85%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	43.49%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

208

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	48.21%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.01%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.38%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.73%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

209

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.49%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.61%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.78%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.55%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

215

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.05%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.42%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	19.72%	Poor	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	23.07%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

230

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.23%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	38.38%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.13%	Poor	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	18.05%	Poor	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

247

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	42.43%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.03%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.44%	Poor	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.73%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

251

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.16%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.79%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.91%	Poor	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.37%	Poor	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

275

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.80%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	18.36%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

317

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.68%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.25%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.42%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.52%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

344

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	55.10%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.96%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	28.25%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	33.54%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

349

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.28%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.16%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	31.31%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.86%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.86%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.32%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.35%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	16.81%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.27%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.05%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.16%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.12%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	33.11%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.21%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.15%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.54%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.13%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.50%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.28%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	20.61%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.10%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	41.91%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.81%	Poor	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.60%	Poor	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad3

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	52.82%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.65%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.98%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.86%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

107

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.79%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

1373

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.17%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.54%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.72%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

1405

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.69%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.63%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

1417

CDS

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.53%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.14%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.01%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

1432

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.91%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.24%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.50%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.01%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

1433

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.36%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.49%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.71%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

1452

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.97%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.23%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.77%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.59%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

154

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.75%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.20%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.85%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.57%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

156

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.06%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.22%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.20%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.23%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

158

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.63%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.76%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

164

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.27%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.56%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.29%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.88%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

166

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.94%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.99%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

197

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.81%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.85%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

317

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.99%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.96%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

362

CDS

C-to-U

ACA=>AUA

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

376

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

401

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.77%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

416

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.69%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.68%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.64%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

433

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.33%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.28%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.79%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.84%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

437

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.45%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.08%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.38%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.74%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.88%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

449

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.66%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.42%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

CDS

C-to-U

ACU=>AUU

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.62%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.26%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.85%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.79%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.53%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.66%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

819

CDS

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	62.77%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.15%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.13%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.57%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.54%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	23.18%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.81%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.99%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

977

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.33%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.13%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.62%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.55%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4

5'UTR

-7

C-to-U

UUC=>UUU

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	23.26%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.27%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.01%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.87%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4L

110

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.82%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.74%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.81%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4L

179

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.26%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.16%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	18.02%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.88%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad4L

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.17%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.26%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.71%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1183

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.11%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.11%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.22%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.63%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1490

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.84%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.92%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1494

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.30%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	28.74%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	52.70%	Medium	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.23%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1550

CDS

C-to-U

ACA=>AUA

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.79%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1567

CDS

C-to-U

CUG=>UUG

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.63%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.58%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	64.41%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.93%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1580

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.57%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.75%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.75%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1589

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.90%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.79%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1665

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.16%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.97%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.99%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.44%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1859

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.84%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.52%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1895

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1900

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.50%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.75%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1901

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.51%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.35%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.73%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1916

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.25%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.00%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.79%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.65%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

1918

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.64%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.02%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.66%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad5

2005

CDS

C-to-U

CAA=>UAA

Q=>Stop codon

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.10%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	13.83%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.30%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.55%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

-3

CDS

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	25.51%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.53%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	27.15%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.99%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

138

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	31.93%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.26%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	18.44%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.29%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

146

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.94%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.56%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	62.34%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

159

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	38.46%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.91%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.60%	Poor	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.45%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

161

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.72%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.60%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.93%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.99%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

169

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.05%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.56%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.58%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

191

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.60%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.26%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.31%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.97%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

196

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	16.54%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.02%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.94%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.12%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.51%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.86%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	37.97%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.68%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.43%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.28%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.46%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.98%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

446

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.31%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.32%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.27%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.92%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

569

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.21%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.92%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.77%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.60%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.37%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.13%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.43%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.33%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.10%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.95%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.28%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.11%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad6

5'UTR

-45

C-to-U

CCC=>UCC

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.98%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.03%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.36%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	12.06%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

1088

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.84%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.77%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.84%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.67%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

1103

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.42%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.96%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

1124

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.96%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.38%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

137

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.63%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.65%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.73%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

209

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.91%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.84%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.58%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

224

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.79%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.06%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

244

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.84%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.52%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

251

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.92%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

255

CDS

C-to-U

GCC=>GCU

A=>A

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	34.46%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.85%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	48.41%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.82%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

315

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.96%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

335

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

344

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.99%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.97%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.51%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

353

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.99%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.96%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.69%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.41%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.71%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

383

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.83%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.95%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.91%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.74%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.91%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

445

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.45%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.75%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.62%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

533

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.27%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.08%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.02%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.89%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

578

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.67%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.89%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.90%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

679

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.21%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.69%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.06%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

698

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.97%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.29%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.63%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

724

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.98%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.80%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

734

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.26%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.45%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.45%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.59%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

739

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.81%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.91%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.68%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.41%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

769

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	100.00%	Complete	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.68%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.47%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.73%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.78%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.96%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.68%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

836

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.93%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.81%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.82%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.87%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

926

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.88%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.94%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.65%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.75%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

973

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.62%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.71%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.57%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.40%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

CDS

C-to-U

AAC=>AAU

N=>N

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.54%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.35%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.70%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.14%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad7

5'UTR

-7

C-to-U

UUC=>UUU

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.93%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.55%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	29.42%	Low	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	34.07%	Low	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

111

CDS

C-to-U

UAC=>UAU

Y=>Y

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.18%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	14.39%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.87%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	18.98%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

113

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.45%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.00%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.20%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.43%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.81%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.66%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.75%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.54%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

167

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.18%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.44%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.17%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.40%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

190

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.14%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.97%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.40%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.03%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

223

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.49%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.55%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.52%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.25%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

298

CDS

C-to-U

CCG=>UCG

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.13%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.88%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.07%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.19%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

311

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.31%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.48%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.62%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.24%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

328

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.78%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.46%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.50%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

356

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.72%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.75%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.80%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.13%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

368

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.28%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.90%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.27%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.43%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

398

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.47%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.11%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.46%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.56%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

nad9

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.25%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.79%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.35%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.77%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

184

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.30%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.79%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.70%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.86%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

225

CDS

C-to-U

GGC=>GGU

G=>G

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.60%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.48%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.48%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.89%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

227

CDS

C-to-U

ACC=>AUC

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.95%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.49%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.01%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.39%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

228

CDS

C-to-U

ACC=>ACU

T=>T

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	38.78%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.38%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	28.29%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.17%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

287

CDS

C-to-U

ACA=>AUA

T=>I

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.96%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.65%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.11%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.09%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

355

CDS

C-to-U

CUC=>UUC

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.80%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.81%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.43%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.65%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

444

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	62.41%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.61%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.82%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.90%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

458

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.93%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.61%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.02%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.28%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

CDS

C-to-U

CUC=>CUU

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.47%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.61%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.33%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.71%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

524

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.67%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.05%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.21%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.50%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

530

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.80%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.60%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.54%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.99%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

CDS

C-to-U

CAG=>UAG

Q=>Stop codon

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.08%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.65%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.44%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.14%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rpl16

CDS

C-to-U

UGC=>UGU

C=>C

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.83%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.57%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.58%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.20%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps1

107

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.20%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.23%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	28.98%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	36.97%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps1

377

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.21%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.34%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.65%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.55%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps1

505

CDS

C-to-U

CAA=>UAA

Q=>Stop codon

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.65%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.07%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	21.78%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.47%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps12

196

CDS

C-to-U

CAC=>UAC

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.46%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.94%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.69%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.38%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps12

221

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.38%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.05%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.93%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	55.54%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps12

269

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.06%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.77%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.26%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.07%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps12

284

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.40%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.78%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.45%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.08%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps12

289

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.09%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	36.24%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.99%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.02%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps12

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.91%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.87%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	33.98%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.70%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps12_ct

Intron

418

C-to-U

CAA=>UAA

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.15%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.45%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	39.39%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.03%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps13

100

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.73%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.58%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.89%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.82%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps13

256

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.96%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.22%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	43.46%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	48.74%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps13

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.29%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.31%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.06%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps13

287

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.23%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	65.96%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.52%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	53.62%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps13

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.84%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.71%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.26%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.85%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps13

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	45.25%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.64%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	36.62%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.36%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2A

200

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.78%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.03%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.67%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.45%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2A

449

CDS

C-to-U

GCA=>GUA

A=>V

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.56%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	55.65%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.08%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.47%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2A

514

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.13%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.30%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	90.77%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.41%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2A

541

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.19%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.66%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.42%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.71%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2A

548

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.11%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.70%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.59%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.82%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2B

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.14%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.19%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	44.12%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.72%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2B

206

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.72%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	85.89%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	73.95%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.72%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2B

455

CDS

C-to-U

GCA=>GUA

A=>V

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.49%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.95%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	94.04%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.17%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2B

510

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.76%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.50%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	19.84%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	31.89%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2B

526

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	8.67%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	10.83%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	9.04%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	15.95%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2B

550

CDS

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.24%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.77%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.76%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.88%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps2B

557

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.30%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	93.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.92%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.14%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

1019

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.00%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	69.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.78%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.08%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

1066

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	19.78%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	19.77%	Poor	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	41.83%	Medium	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.95%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

1576

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.12%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.96%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	89.45%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.46%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

1607

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.09%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.37%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.85%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.81%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

1621

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.95%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.16%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.85%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.73%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

314

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	22.55%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	20.38%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	43.21%	Medium	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	43.94%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

497

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	32.41%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	31.05%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	51.51%	Medium	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.41%	Medium	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

503

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.52%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.73%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.97%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.48%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.58%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	97.01%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.86%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	98.66%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.26%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.74%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	57.00%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.13%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

707

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	34.34%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	34.51%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.01%	High	Increased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.44%	High	Increased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

878

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.10%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.87%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	16.26%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	17.84%	Poor	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps3

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.86%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.42%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.45%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	99.49%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

1031

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.11%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.12%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.62%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	92.30%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

1040

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	84.99%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.42%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.44%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.62%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

1045

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.92%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.65%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.88%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.24%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

164

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.61%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	64.67%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.17%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.18%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

278

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	87.59%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.55%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.03%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	95.83%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

290

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.54%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.43%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	64.62%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.97%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

335

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.58%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.64%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.85%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	91.06%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.38%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	71.76%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	67.20%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	64.53%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

482

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	66.09%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	58.90%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	68.58%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	72.11%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.05%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.26%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	88.01%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	96.11%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

549

CDS

C-to-U

UUC=>UUU

F=>F

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	43.85%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.91%	Medium	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	23.55%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	35.18%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

870

CDS

C-to-U

ACC=>ACU

T=>T

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	16.76%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	24.63%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

915

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	83.17%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.60%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	76.12%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	86.69%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

944

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.98%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.41%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	56.70%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	59.21%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

955

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	80.44%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	74.93%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	78.85%	High	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	81.62%	High	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

980

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	79.06%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	75.34%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	47.85%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	50.47%	Medium	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps4

5'UTR

-9

C-to-U

CAA=>UAA

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	49.19%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.62%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	40.31%	Medium	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	55.58%	Medium	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps7

277

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	54.65%	Medium	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	61.13%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	31.76%	Low	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.39%	Low	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps7

332

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	77.31%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	82.97%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	60.39%	High	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	63.76%	High	Decreased	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps7

520

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.99%	Poor	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	36.95%	Low	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	11.72%	Poor	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	20.87%	Low	Similar	31374076

Zea mays

GRMZM5G849971

Mitochondrion

rps7

5'UTR

-114
-68

C-to-U

CCU=>UCU
UCG=>UUG

NA=>NA

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.84%	Low	None	31374076
W22	WT-1	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	64.09%	High	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	30.98%	Low	None	31374076
W22	WT-2	Control	Control	Control	Normal	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	70.86%	High	None	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	26.24%	Low	Similar	31374076
W22	emp21-1	Mutator (Mu) insertion	A Mu insertion at +280 bp from the translation start codon in GRMZM5G849971	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	52.32%	Medium	Decreased	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	23.34%	Low	Similar	31374076
W22	emp21-2	Mutator (Mu) insertion	The Mu element is inserted at +608 bp from the GRMZM5G849971 translation start codon	Heterozygous	Empty pericarp	Kernel	12 DAP	STS-PCRseq; Direct sequencing of RT-PCR amplicons	46.97%	Medium	Decreased	31374076

Editing Factor:	EMP21
Synonym:	Empty Pericarp21
Description:	From a scan of 35 mitochondrial transcripts produced by the Emp21 loss-of-function mutant, the C-to-U editing was found to be abolished at five sites (nad7-77, atp1-1292, atp8-437, nad3-275 and rps4-870), while reduced at 76 sites in 21 transcripts; In consequence, the mutant became deficient with respect to the assembly and activity of mitochondrial complexes I and V; As six of the decreased editing sites in emp21 overlap with the affected editing sites in emp5-1, and the editing efficiency at rpl16-458 showed a substantial reduction in the emp21-1 emp5-4 double mutant compared with the emp21-1 and emp5-4 single mutants, we explored their interaction; These results indicate that EMP21 is a novel PPR-DYW protein required for the editing of ~17% of mitochondrial target Cs, and the editing process may involve an interaction between EMP21 and ZmMORF8 (and probably other proteins)
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	MORF8
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	GRMZM5G849971 (Zea mays)
Edited Gene(s):	atp1 atp4 atp6 atp8 atp9 ccmB ccmC ccmFc ccmFn cob cox1 cox2 cox3 matR mttB nad1 nad2 nad3 nad4 nad4L nad5 nad6 nad7 nad9 rpl16 rps1 rps12 rps12_ct rps13 rps2A rps2B rps3 rps4 rps7
Editing Type(s):	C-to-U (1972)
Publication(s):	[1] Empty Pericarp21 encodes a novel PPR-DYW protein that is required for mitochondrial RNA editing at multiple sites, complexes I and V biogenesis, and seed development in maize., PLoS genetics, 2019. [PMID=31374076]

Summary

Editing Details