NUWA - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G074599

Mitochondrion

atp1

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	54.92%	Medium	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	5.08%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

atp4

360

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	84.75%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.37%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

atp4

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.93%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	12.54%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

atp4

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.93%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	50.85%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

atp6

279

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	51.86%	Medium	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

atp8

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.22%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	30.51%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

atp9

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	91.53%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	6.78%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmB

286

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	71.19%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	47.46%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmB

304

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.80%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	4.07%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmB

338

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	74.58%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	10.85%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmB

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	73.56%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	6.78%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmB

551

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.80%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	28.47%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmB

554

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.80%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	10.85%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

-16

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	16.27%	Poor	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

1145

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	70.85%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	29.15%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

1244

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	81.36%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.37%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

1300

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	69.49%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	5.08%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

146

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	97.29%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

282

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	13.90%	Poor	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

306

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	71.19%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	46.44%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	94.92%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	58.98%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFc

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.90%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	30.51%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

137

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.56%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	44.07%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

1408

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.22%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.71%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

1469

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	92.20%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	71.19%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

1489

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	71.19%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.08%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

176

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.56%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	64.41%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

190

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	85.42%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.10%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

1918

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	94.92%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	33.56%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

410

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.22%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	55.93%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

417

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.25%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	26.10%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

ccmFn

514

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	34.92%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.39%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cob

1124

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.02%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	6.56%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox2

162

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	37.70%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.93%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox2

259

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.02%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	66.56%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox2

482

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.87%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	31.80%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox2

587

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	92.79%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	32.13%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox2

638

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	32.13%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.30%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox2

704

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	94.43%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	18.36%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox3

245

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	81.64%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox3

257

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	81.64%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	4.59%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox3

314

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	81.31%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	42.30%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

cox3

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	61.97%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	7.21%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Plastid

matK

1258

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	78.43%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	57.14%	Medium	Decreased	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	61.90%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

matR

1895

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	72.05%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	41.21%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

matR

296

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	63.98%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.94%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

mttB

139

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	16.27%	Poor	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

mttB

532

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	34.58%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	10.51%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad1

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.27%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	22.48%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad1

308

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.42%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.72%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad1

580

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.42%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	48.99%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad1

674

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.14%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	63.69%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad1

743

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.14%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	65.99%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad1

898

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.42%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	62.25%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad1

928

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.42%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	52.45%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad2

367

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.14%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	14.41%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad2

453

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	36.02%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.88%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad3

317

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	33.14%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad3

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	29.11%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	7.49%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad4

107

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	81.84%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad4

416

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.85%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	50.43%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad4

433

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.27%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	48.99%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad4

449

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.56%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	40.06%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad4

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.14%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	30.26%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad4

819

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.36%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad4L

110

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	34.87%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad5

1665

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	72.91%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.65%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad5

1916

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	97.69%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	68.88%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad6

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	78.96%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	26.22%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad6

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	86.17%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad6

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	88.47%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	61.96%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

-7

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	30.26%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

1088

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	96.25%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	37.75%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

224

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.27%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	16.43%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

344

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.42%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	6.92%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.85%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	33.43%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

739

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.42%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	47.55%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

769

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.85%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	45.53%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

836

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.14%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	75.50%	High	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad7

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	50.43%	Medium	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	18.44%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

nad9

328

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	51.30%	Medium	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	12.10%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Plastid

ndhA

473

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Plastid

ndhA

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Plastid

ndhB

467

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	86.36%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	59.63%	Medium	Decreased	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	54.23%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Plastid

ndhD

878

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	92.41%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	44.93%	Medium	Decreased	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	43.97%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rpl16

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	88.52%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	42.62%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rpl16

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.74%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	45.57%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Plastid

rpl2

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	39.36%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Plastid

rpoB

617

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	61.07%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Plastid

rpoC2

2774

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	73.44%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	17.24%	Poor	Decreased	38327059
NA	zmnuwa-1; zmnuwa-2	Nucleotide insertion; Nucleotide deletion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant; A 94 bp deletion from +197 to +290 bp from the start codon in the ZmNUWA gene	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	16.91%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps12

284

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	69.84%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	27.87%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps12

289

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	40.98%	Medium	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps12

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.54%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.28%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps12_ct

418

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	90.16%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps13

100

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	84.26%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	5.90%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps2A

102

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	40.98%	Medium	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.95%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps2B

108

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.90%	Low	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps2B

557

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	91.48%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	52.79%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps3

1576

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	83.60%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	31.15%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps3

1621

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	81.31%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	26.23%	Low	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps3

314

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	84.59%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	5.57%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps3

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	97.05%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	10.16%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps4

278

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	90.16%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	49.84%	Medium	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps4

290

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	79.67%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	14.75%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps4

335

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	86.23%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.30%	Poor	Decreased	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps4

879

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	10.49%	Poor	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G074599

Mitochondrion

rps4

980

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	65.25%	High	None	38327059
NA	zmnuwa-1	Nucleotide insertion	A "T" nucleotide insertion at +280 bp within the ZmNUWA gene in the zmnuwa-1 mutant	Heterozygous; Recessive	Kernels developed very small transparent endosperm, but the embryo could not be found; Paraffin sectioning shows that the endosperm is arrested at the cellularization stage, whereas the embryo is probably too tiny to be detected in the zmnuwa-1 mutant	Embryo; Endosperm	12 DAP	STS-PCR-seq	4.26%	Poor	Decreased	38327059

Editing Factor:	NUWA
Synonym:	EMB1796; EMBRYO DEFECTIVE 1796; AtNUWA
Description:	NUWA and DYW2 Are Dually Targeted to Chloroplast and Mitochondria; NUWA and DYW2 Proteins Are Required for Embryo Development; DYW2 and NUWA Proteins Are Functionally Linked and Involved in Editing of Chloroplast and Mitochondria Transcripts; DYW2 and NUWA Are Required for Editing by PPR-E+
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	P
Physical Interaction:	ZmDYW2A; ZmDYW2B; ZmMORF1; ZmMORF2; ZmMORF8; ZmMORF9
Construct Structure:	P
Gene ID & Species:	GRMZM2G074599 (Zea mays)
Edited Gene(s):	atp1 atp4 atp6 atp8 atp9 ccmB ccmFc ccmFn mttB nad1 nad2 nad3 nad4 nad4L nad5 nad6 nad7 nad9 matR cob cox2 cox3 rps2A rps2B rps3 rps4 rps12 rps12_ct rps13 rpl16 ndhA ndhB ndhD rpl2 rpoB rpoC2 matK
Editing Type(s):	C-to-U (222)
Publication(s):	[1] Multiple factors interact in the editing of the PPR-E+ targeted sites in maize mitochondria and plastids, Plant Communications, 2024. [doi:10.1016/j.xplc.2024.100836]

Summary

Editing Details