DYW2A - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G017821

Mitochondrion

atp1

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	53.85%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.08%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

atp4

360

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	84.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	2.31%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

atp4

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	96.93%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	11.54%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

atp4

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	55.77%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

atp6

279

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	51.15%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

atp8

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	94.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	33.46%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

atp9

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	91.92%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	7.31%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmB

286

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	80.38%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	53.85%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmB

304

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	79.62%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.85%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmB

338

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	85.77%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	13.46%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmB

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	78.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	7.69%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmB

551

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	80.00%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	32.69%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmB

554

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	77.31%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	15.00%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFc

-16

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	6.54%	Poor	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFc

1244

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	57.69%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFc

1300

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	73.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFc

146

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	69.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	1.92%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFc

282

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	15.38%	Poor	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFc

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	81.15%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	16.15%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

137

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.85%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	42.31%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

1408

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	94.62%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.08%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

1489

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	74.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	22.31%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

176

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.38%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	66.54%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

190

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	89.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.00%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

1918

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.38%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	35.38%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

410

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.85%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	59.62%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

417

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.00%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	28.85%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

ccmFn

514

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	35.38%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.85%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cob

1124

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	7.69%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox2

148

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	20.38%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox2

162

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	43.46%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.46%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox2

259

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.31%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox2

482

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	78.85%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	24.23%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox2

587

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	95.77%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.77%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox2

638

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	45.00%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.85%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox2

704

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	96.92%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	16.92%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox3

245

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	87.69%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	1.92%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox3

257

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	87.69%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	5.00%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox3

314

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	87.69%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	43.85%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

cox3

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	70.77%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	8.46%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Plastid

matK

1258

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	86.67%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	62.77%	High	Decreased	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	62.50%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

matR

1895

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	76.92%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	43.46%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

matR

296

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	60.38%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	17.69%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

mttB

139

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	15.77%	Poor	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

mttB

532

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	39.23%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	12.69%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad1

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.46%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	35.00%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad1

308

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.85%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	71.15%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad1

580

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.62%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	55.38%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad1

674

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	70.77%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad1

743

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	73.08%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad1

898

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	70.38%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad1

928

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	61.54%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad2

367

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	99.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	13.08%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad2

453

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	39.62%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.46%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad3

317

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	38.46%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad3

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	33.46%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	5.38%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad4

107

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	82.31%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad4

416

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	42.31%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad4

433

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	96.54%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	40.38%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad4

449

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	38.08%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad4

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	97.31%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	29.62%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad4

819

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	19.23%	Poor	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad4L

110

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.00%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad5

1665

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	69.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	15.77%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad5

1916

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	96.92%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	57.69%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad6

146

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	91.54%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	65.38%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad6

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	88.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	24.23%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad6

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.46%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad6

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	96.92%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	65.00%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad6

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	96.15%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	51.92%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

-7

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	32.69%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

1088

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	33.46%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

224

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	13.46%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

344

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	7.69%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	35.77%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

739

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	46.15%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

769

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	42.69%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

836

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	98.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	69.23%	High	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad7

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	60.38%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	22.69%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad9

190

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	73.46%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	51.92%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

nad9

328

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	72.31%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	9.62%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Plastid

ndhA

473

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Plastid

ndhA

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	100.00%	Complete	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Plastid

ndhB

467

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	72.92%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	61.11%	High	Decreased	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	50.00%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Plastid

ndhD

878

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	75.00%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	35.94%	Low	Decreased	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	50.00%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rpl16

228

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	69.23%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	39.62%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rpl16

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	85.38%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	40.38%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rpl16

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	92.31%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	40.38%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Plastid

rpl2

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	58.65%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	28.85%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Plastid

rpoB

617

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	44.04%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Plastid

rpoC2

2774

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	63.30%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	15.65%	Poor	Decreased	38327059
NA	zmdyw2a-2::zmdyw2b-2	Nucleotide deletion	zmdyw2a-2 carries a 556 bp deletion from 99 to 654 bp from the start codon in ZmDYW2A; zmdyw2b-2 carries a 17 bp deletion from 81 to 97 bp, and a 3 bp deletion from 648 to 650 bp from the start codon	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	22.32%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps12

284

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	69.62%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	45.77%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps12

289

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	44.23%	Medium	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps12

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	64.62%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	6.54%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps12_ct

418

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	82.31%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps13

100

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	84.62%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	7.69%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps2A

102

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	30.00%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	3.84%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps2B

108

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.00%	Low	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps2B

557

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	86.15%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	50.77%	Medium	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps3

1576

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.69%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	34.23%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps3

1621

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	67.69%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.77%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps3

314

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	83.08%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps3

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	93.85%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	16.54%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps4

290

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	76.54%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	25.77%	Low	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps4

335

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	83.46%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	8.85%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps4

879

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	14.62%	Poor	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps4

980

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	62.69%	High	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	5.77%	Poor	Decreased	38327059

Zea mays

GRMZM2G017821

Mitochondrion

rps7

489

CDS

MaizeGDB database (www.maizegdb.org)

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	Normal	Embryo; Endosperm	12 DAP	STS-PCR-seq	17.31%	Poor	None	38327059
NA	zmdyw2a-1::zmdyw2b-1	Nucleotide deletion	zmdyw2a-1 carries a 4 bp deletion from 653 to 656 bp; zmdyw2b-1 carries a "T" nucleotide deletion at 650 bp	Knockout	Genetic analysis shows the single mutant of either ZmDYW2A or ZmDYW2B does not differ from the wild-type in plant growth and seed development; The zmdyw2a-1::zmdyw2b-1 double mutants showed a similar phenotype to zmnuwa, with an invisible embryo and very small translucent endosperm at 12 DAP	Embryo; Endosperm	12 DAP	STS-PCR-seq	0.00%	Unedited	Absent	38327059

Editing Factor:	DYW2A
Synonym:	ZmDYW2A
Description:	The zmdyw2a and zmdyw2b single mutants are normal, but the zmdyw2a::zmdyw2b and zmnuwa mutants are severely arrested in seed development; ZmNUWA, ZmDYW2A, and ZmDYW2B are dual-localized in mitochondria and plastids; Loss of ZmDYW2A:ZmDYW2B affects almost the same set of sites targeted by PPR-E+ proteins; ZmNUWA and ZmDYW2A/2B interact with ZmMORF1, ZmMORF8, ZmMORF2, and ZmMORF9; and ZmOZ1 interacts with ZmORRM1, ZmDYW2A, ZmDYW2B, ZmMORF8, and ZmMORF9
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	ZmNUWA ; ZmDYW2B; ZmMORF1; ZmMORF2; ZmMORF8; ZmMORF9; ZmOZ1
Construct Structure:	DYW
Gene ID & Species:	GRMZM2G017821 (Zea mays)
Edited Gene(s):	atp1 atp4 atp6 atp8 atp9 ccmB ccmFc ccmFn mttB nad1 nad2 nad3 nad4 nad4L nad5 nad6 nad7 nad9 matR cob cox2 cox3 rps2A rps2B rps3 rps4 rps7 rps12 rps12_ct rps13 rpl16 ndhA ndhB ndhD rpl2 rpoB rpoC2 matK
Editing Type(s):	C-to-U (224)
Publication(s):	[1] Multiple factors interact in editing of PPR-E+-targeted sites in maize mitochondria and plastids., Plant communications, 2024. [PMID=38327059]

Summary

Editing Details