| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp1
|
1499 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 97.34% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 86.78% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp1
|
30 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 29.33% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 3.37% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp4
|
360 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 92.59% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 76.30% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp4
|
40 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 91.69% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 68.04% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp4
|
56 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 98.51% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 65.87% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp4
|
59 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 98.92% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 84.01% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp4
|
71 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.34% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 84.04% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp4
|
76 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.46% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 86.49% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp6
|
1050 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 16.51% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 1.26% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp6
|
1124 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 98.26% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 87.17% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp6
|
1132 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 95.43% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 75.69% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp6
|
1139 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.64% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 73.50% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp6
|
1148 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 97.78% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 83.47% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp6
|
738 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 50.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 90.58% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 42.21% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp6
|
953 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 71.83% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 82.16% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp8
|
123 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 89.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 15.00% | Poor | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 90.60% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 17.75% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp8
|
200 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.30% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 75.09% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp8
|
436 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 71.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 23.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 87.39% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 14.65% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp8
|
437 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.28% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 74.66% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp8
|
58 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.27% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 83.44% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp9
|
191 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 92.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 72.00% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp9
|
20 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 77.00% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
atp9
|
212 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 50.00% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
128 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.63% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 50.15% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
137 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 75.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 93.42% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 39.25% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
149 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.99% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 81.39% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
183 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 75.57% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 57.08% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
194 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 84.98% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 70.16% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
28 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 95.39% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 43.72% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
286 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 90.46% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 74.80% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
337 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 73.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 38.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 57.01% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 28.13% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
367 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.55% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 84.14% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
379 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 74.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 87.89% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 37.99% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
391 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 19.85% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 9.28% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
396 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 11.70% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.34% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
43 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 95.10% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 67.60% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
512 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.48% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 85.44% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
566 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 92.55% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 78.40% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmB
|
583 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 41.62% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 28.09% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
103 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 61.32% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 38.69% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
115 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 34.10% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 11.52% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
179 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 65.95% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 39.23% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
227 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 69.33% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 35.86% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
331 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 20.08% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 33.03% | Low | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
399 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 24.48% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 6.54% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
400 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 16.03% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 2.37% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
436 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 39.86% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 10.72% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
446 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 60.98% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 78.05% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
451 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 40.47% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 22.53% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
463 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 39.79% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 27.56% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
473 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 38.87% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 23.66% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
497 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 55.53% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 38.66% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
499 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 51.59% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 28.94% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
521 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 63.83% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 39.18% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
548 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 36.29% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 54.54% | Medium | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
568 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 39.97% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 8.18% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
608 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 35.86% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 65.83% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
614 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 27.05% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 11.95% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
624 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 20.76% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 4.52% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
665 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 55.07% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 24.95% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmC
|
673 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 68.34% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 19.49% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
1145 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 93.27% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 80.04% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
160 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 87.13% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 56.75% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
306 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 95.13% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 80.59% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
38 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 93.36% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 71.05% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
39 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 21.88% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 9.98% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
50 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.83% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 78.27% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFc
|
543 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 0.00% | Unedited | Absent | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 89.45% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 22.31% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
906 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 74.07% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 17.04% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFC
|
907 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 11.84% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 1.69% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
1342 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 74.13% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 34.57% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
1492 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 23.19% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 4.61% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
1493 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 64.73% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 14.58% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
190 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 83.87% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 58.34% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
302 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 81.47% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 70.80% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
318 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 20.12% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.93% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
401 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 92.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 34.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 78.68% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 22.75% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
595 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 87.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 7.00% | Poor | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 56.64% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 8.84% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
743 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 84.18% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 38.05% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
76 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 83.34% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 59.09% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
77 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 45.95% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 23.86% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
824 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 85.09% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 74.44% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
ccmFn
|
839 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 84.98% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 59.19% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cob
|
1098 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 72.22% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 40.63% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cob
|
564 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 88.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 20.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 81.22% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 18.92% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cob
|
853 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.04% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 88.73% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cob
|
908 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 98.54% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 86.99% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox1
|
1433 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.65% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 84.01% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox1
|
15 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 42.75% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 14.55% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox2
|
144 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 85.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 16.00% | Poor | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 81.18% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 34.20% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox2
|
148 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 14.50% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 1.27% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox2
|
31 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 66.73% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 49.93% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox2
|
482 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 84.53% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 43.52% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox2
|
620 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 77.81% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 92.34% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox2
|
638 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 62.07% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 78.28% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox3
|
422 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 83.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 66.00% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
cox3
|
69 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 84.24% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 72.01% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
1730 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 86.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 57.00% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
1751 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 63.12% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 81.34% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
1785 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 26.00% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 0.00% | Unedited | Absent | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
1885 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 73.91% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 83.97% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
1895 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 61.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 81.59% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 56.82% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
1905 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 46.00% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 0.00% | Unedited | Absent | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 32.79% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 7.89% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
295 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 24.88% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 13.27% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
296 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 56.69% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 36.64% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
matR
|
92 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 30.56% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 7.86% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
138 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 19.13% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 5.42% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
155 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 72.87% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 52.68% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
251 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 76.69% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 44.88% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
289 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 77.56% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 35.53% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
358 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 75.66% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 46.88% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
371 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 48.93% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 16.49% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
403 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 73.12% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 50.07% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
464 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 79.79% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 55.08% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
468 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 34.70% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 2.19% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
580 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 79.84% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 29.19% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
mttB_orfX
|
637 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 80.80% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 28.55% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
492 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 85.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 97.32% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 49.62% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
536 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.93% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 84.60% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
537 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 31.67% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 48.06% | Medium | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
635 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 82.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.54% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 71.18% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
734 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.20% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 59.36% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
779 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 83.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 40.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 86.64% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 54.39% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
823 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 67.50% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 75.95% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 32.00% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad1
|
937 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.88% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 49.76% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
1028 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 97.77% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 68.34% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
1058 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.78% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 62.19% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
1408 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.61% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 83.06% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
1409 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.39% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 35.95% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
1416 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.31% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 87.23% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
1457 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 44.53% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 4.64% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
356 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 72.72% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 61.09% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
394 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.88% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 65.49% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
523 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.35% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 87.24% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad2
|
800 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 69.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.34% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 47.33% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
137 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 82.02% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 51.90% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
137, 138 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 90%; 39% | High; Low | None; None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 59%; 24% | Medium; Lo | Decreased; | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
146 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 82.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 34.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 65.78% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 25.83% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
185 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 72.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 83.82% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 63.43% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
190 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 91.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 46.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 69.08% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 35.13% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
208 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 62.15% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 44.86% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
208, 209 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100%; 100% | Complete; | None; None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 50%; 60% | Medium; Me | Decreased; | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
209 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 71.27% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 53.71% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
215 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 89.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 50.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 57.84% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 40.60% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
230 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 79.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 50.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 49.07% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 33.96% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
247 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 86.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 39.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 55.98% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 30.98% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
251 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 73.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 40.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 46.68% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 32.00% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
275 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 77.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 42.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 46.57% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 34.11% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
317 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 54.00% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
344 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 44.00% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
349 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 36.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 57.68% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 44.63% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
39 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 54.00% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 28.00% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
44 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 73.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 64.00% | High | Similar | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
61 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 61.51% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 42.15% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
61, 62 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 70%; 72% | High; High | None; None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 46%; 24% | Medium; Lo | Decreased; | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad3
|
62 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 57.67% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 18.46% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad4
|
1432 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.50% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 52.84% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad4
|
154 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.21% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 66.07% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad4
|
164 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 98.99% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 58.63% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad4L
|
100 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 75.56% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 31.42% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad4L
|
188 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 64.88% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 42.33% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad4L
|
197 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 70.47% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 47.29% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad4L
|
95 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 73.39% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 26.81% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad5
|
1550 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 80.33% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad5
|
1567 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 36.58% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 2.31% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad5
|
1895 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.53% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 60.35% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad5
|
1900 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.55% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 66.74% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad6
|
138 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 70.40% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 52.58% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad6
|
146 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 39.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 83.70% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 31.97% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad6
|
159 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 35.89% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.91% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad6
|
161 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 92.44% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 37.97% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad6
|
26 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 93.28% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 79.12% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad6
|
446 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 95.91% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 85.81% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad7
|
335 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.89% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 86.59% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad7
|
344 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.93% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 75.72% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad7
|
353 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.79% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 68.15% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad7
|
531 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 8.52% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 33.60% | Low | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad7
|
533 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 99.43% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 77.32% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad7
|
99 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 70.24% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 55.21% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
111 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 25.00% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 0.00% | Unedited | Absent | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 32.48% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 6.97% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
113 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 94.75% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 71.06% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
167 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 98.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 81.17% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
190 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 77.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 89.24% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 44.12% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
223 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.24% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 72.41% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
298 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 95.69% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 77.37% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
311 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 97.84% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 69.12% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
328 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.44% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 76.61% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
356 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 93.01% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 64.85% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
368 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.20% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 68.62% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
398 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 97.23% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 68.82% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
nad9
|
92 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 92.97% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 73.31% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
ndhA
|
50 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 56.75% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 86.95% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
ndhB
|
1481 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 29.78% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 46.77% | Medium | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
ndhB
|
586 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 83.98% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 72.73% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
ndhB
|
611 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 48.84% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 23.03% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
ndhF
|
62 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 14.96% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 58.04% | Medium | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rpl16
|
228 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 44.00% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 22.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 48.67% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 17.98% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rpl16
|
444 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 67.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 37.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 62.86% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 44.44% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rpl16
|
524 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 83.25% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 93.36% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
rpl20
|
308 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 77.29% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 94.74% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
rpoB
|
560 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 32.79% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 62.28% | High | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps1
|
107 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 82.77% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 48.39% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps1
|
509 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 23.17% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 6.50% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps12
|
196 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 34.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 95.59% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 67.76% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps12
|
221 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 77.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 55.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 90.64% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 77.28% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps12
|
269 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 35.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 91.43% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 61.37% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps12
|
284 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 61.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 92.86% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 63.44% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps12
|
289 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 84.14% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 65.27% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps12
|
71 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 67.00% | High | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 90.96% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 78.38% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps13
|
26 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 73.94% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 57.80% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps13
|
56 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 50.00% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 42.00% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2A
|
102 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 49.43% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 9.77% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2A
|
200 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 73.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 45.00% | Medium | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 97.01% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 76.37% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
108 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 23.87% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.36% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
15 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 25.26% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.00% | Unedited | Absent | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
200 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 73.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 45.00% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
206 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 74.44% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 52.39% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
431 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 0.05% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 42.66% | Medium | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
455 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 100.00% | Complete | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 41.00% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
510 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 18.08% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.00% | Unedited | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps2B
|
783 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 0.03% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 39.47% | Low | Increased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps3
|
1066 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 19.63% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 7.54% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps3
|
314 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 70.64% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 56.93% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps3
|
497 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 37.31% | Low | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 8.06% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps3
|
69 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 71.09% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 12.94% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps3
|
851 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 86.00% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 31.00% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps3
|
878 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 50.00% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | Sanger sequencing of targeted RT-PCR products | 29.00% | Low | Decreased | 41771660 | | NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 13.35% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.71% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
1031 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 67.22% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 38.45% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
1045 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 62.03% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 48.86% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
278 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 83.03% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 70.45% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
290 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 69.06% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 56.34% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
335 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 81.87% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 68.41% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
482 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 49.01% | Medium | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 27.66% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
49 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 87.72% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 66.50% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
549 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 16.11% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 1.15% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
870 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 11.83% | Poor | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 0.81% | Poor | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
915 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 73.41% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 47.31% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps4
|
980 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 67.89% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 30.55% | Low | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps7
|
277 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 89.60% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 51.50% | Medium | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Mitochondrion |
rps7
|
332 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 96.30% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 83.63% | High | Decreased | 41771660 |
|
| Zea mays |
GRMZM2G164018 |
Plastid |
rps8
|
182 |
CDS |
W22 |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild type | No treatment | No mutant | Normal | Endosperm | NA | STS-PCR-seq | 63.06% | High | None | 41771660 | | Zmgrp23-mu/Zmgrp23-ems | Zmgrp23-mu/Zmgrp23-ems | A Mu insertion and a point mutation | The Mu transposon in Zmgrp23-mu was inserted within the bZIP domain, while Zmgrp23-ems carried a point mutation in the glutamine-rich region, resulting in a truncation of 61-amino acid residues at the | Heterozygous | Class I (59/98, 60%) exhibited slightly larger endosperm relative to the embryo, with embryonic development arrested at the coleoptilar stage; Class II (29/98, 30%) showed embryos reduced to approximately one-third of endosperm volume and arrested at the transition stage; Class III (10/98, 10%) displayed miniature embryos fully embedded within endosperm tissue | Endosperm | NA | STS-PCR-seq | 93.76% | High | Increased | 41771660 |
|
